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1. The sucrose, raffinose and inulin spaces within the gastrocnemius of the female rat have been estimated at 12·0 ± 2·2%, 10·7 ± 0·6%, and 10·0 ± 0·3% respectively, expressed as a percentage of the total muscle volume.

2. The mucopolysaccharide and hexosamine contents of the gastrocnemius have been found to be inversely dependent upon the weight of the muscle.

3. The possibility that a relation exists between the saccharide spaces and the muscle musopolysaccharide content has been discussed in terms of saccharide molecular radii.

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