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1. The unstirred layer of fluid bounding the posterior surface of the rabbit cornea and contact lens has been directly measured using an optical apparatus.
2. Small polystyrene latex spheres (< 0·25 µ diameter) were used as an indicator because of the excellent light-reflective properties; other indicators were used (e.g. carmine particles) and had a similar behaviour.
3. With the bulk solution unstirred the layer was 350 µ thick on the cornea and 150 µ thick on the contact lens. Following vigorous stirring the layer was reduced to 65 µ on the cornea and < 20 µ on the contact lens.
4. The present direct measurements of the unstirred layers thickness confirm previous indirect measurements.
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