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1. A method for measurement of short-circuit current and for applying a voltage clamp to segments of rat colon in vivo is described.

2. The mucosa behaved as an ohmic resistor of average resistance 154 /cm² although brief transient effects were frequently observed. Tissue resistance was independent of considerable changes in ionic strength and composition of the luminal solution.

3. The short-circuit current averaged 120 µA/cm² in normal rats. Aldosterone intravenously raised the p.d., short-circuit current rising proportionately and tissue resistance being unchanged. The effects of various modifications of the intraluminal solution in respect to composition, hydrostatic pressure and pH were examined. An increase in the osmolality of the luminal solution sufficient to abolish water absorption did not affect p.d. or short-circuit current.

4. The short-circuit current measured with 150 mM-NaCl in the lumen was almost completely accounted for by active Na absorption both in normal and aldosterone-treated rats. The changes in Na efflux rate produced by voltage clamping suggested that only part of Na efflux was due to simple diffusion. With lower [NaCl] in the lumen, the short-circuit current exceeded that atributable to active Na absorption, the discrepancy increasing with reduction of [NaCl].

5. The luminal [Na] at which Na efflux and influx rates were equal was reduced by aldosterone, an effect which is probably responsible for the low stool [Na] of aldosterone treated animals. The significance of this finding in terms of the mode of action of aldosterone is discussed.