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The nucleus linearis intermedius raphe and the nucleus linearis rostralis were stimulated during the perfusion of the anterior horn of the right lateral ventricle of anaesthetized cats. Whereas release of 5-hydroxytryptamine (5-HT) was consistently obtained, there was no release of acetylcholine (ACh).

The independence of the release of 5-HT from that of ACh was seen both during low basal release of ACh (rising base line), and during the period when a plateau of resting release had been reached. It was also demonstrated in experiments in which the same perfusate was examined for both compounds.