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1. Stimulus-secretion phenomena in blood platelets incubated with trypsin or thrombin have been investigated.

2. Secretion occurs in at least two stages: (a) induction, obtained when the platelets are incubated with proteolytic enzymes in the presence of the chelating agent EDTA, and (b) release, which occurs with the subsequent addition of Ca²⁺ to the incubation medium.

3. Ca²⁺ was the only essential divalent cation required for release. Ba²⁺ and Mg²⁺ inhibited release and Sr²⁺ could only partially substitute for Ca²⁺.

4. Blood platelets exhibited recovery from enzymic perturbation by trypsin or thrombin by a mechanism that was temperature dependent, but did not require the synthesis of new protein.

5. The results are discussed in relationship to stimulus—secretion in other cells.