HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Boardman, L. Articles by Polson, J. M. Search for Related Content PubMed PubMed Citation Articles by Boardman, L. Articles by Polson, J. M.

1. HeLa cells were grown in normal and altered growth solutions; the ion contents, volumes, K sensitive ouabain binding, the Na-K-ATPase and the Na and K transport measured.

2. Cells grown in 1 x 10^-4 M ethacrynate or low-K media for 24 hr have a raised [Na]_i, a decreased [K]_i, and an increased ouabain binding. Those grown in low-K also have an increased Na-K-ATPase activity.

3. When cells are put into low-K solutions the [Na]_i initially rises to a high value, and then starts to fall some 8 hours later as the ouabain binding increases, suggesting that these additional sites represent working Na pumps. Flux measurements on low-K cells provide some support for this view.

4. Experiments in which sorbitol replaced [Na]_o showed that the increased ouabain binding and Na-K-ATPase was related to the increase in [Na]_i rather than the decrease in [K]_i and was not due to a non-specific effect of [K]_o change.

5. The protein synthesis inhibitors cycloheximide and puromycin stopped the effect of ethacrynate and low-K solutions on increased ouabain binding. They also decreased the ouabain binding and K influx in normal cells over 24 hr. Cycloheximide had similar effects on Na-K-ATPase in low-K treated and normal cells. These results suggest that protein synthesis is required for the appearance of more ouabain sensitive sites in the cell membrane, both in response to ethacrynate and low-K treatment and for normal replacement during the cell's life.

6. The RNA synthesis inhibitors actinomycin D (AMD) and cordycepin had complex effects on ouabain binding in fresh and ethacrynate treated cells. These inhibitors increased the ouabain binding but decreased the K influx. This discrepancy was due to the appearance of ouabain binding sites with different characteristics from normal sites. A limited investigation of this phenomenon was carried out. Probably AMD stops the normal replacement of sites in the membrane.

7. These results are consistent with the hypothesis that HeLa cells have a system for controlling the number of Na pumps in their membranes. This system responds to the level of [Na]_i within the cell and involves protein synthesis. It is not clear to what extent the nucleus is normally involved in this process.

This article has been cited by other articles:

				X. Zhou, W. Yin, S. Q. Doi, S. W. Robinson, K. Takeyasu, and X. Fan Stimulation of Na,K-ATPase by low potassium requires reactive oxygen species Am J Physiol Cell Physiol, August 1, 2003; 285(2): C319 - C326. [Abstract] [Full Text] [PDF]

				J. F. Hoffman, A. Wickrema, O. Potapova, M. Milanick, and D. R. Yingst Na pump isoforms in human erythroid progenitor cells and mature erythrocytes PNAS, October 29, 2002; 99(22): 14572 - 14577. [Abstract] [Full Text] [PDF]

				X. Liu, L. J. Hymel, and E. Songu-Mize Role of Na+ and Ca2+ in stretch-induced Na+-K+-ATPase alpha -subunit regulation in aortic smooth muscle cells Am J Physiol Heart Circ Physiol, January 1, 1998; 274(1): H83 - H89. [Abstract] [Full Text] [PDF]