HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Brown, D. A. Articles by Scholfield, C. N. Search for Related Content PubMed PubMed Citation Articles by Brown, D. A. Articles by Scholfield, C. N.

1. Na and K contents of isolated rat superior cervical ganglia were measured by flame photometry, and intracellular Na and K concentrations ([Na]_i and [K]_i) calculated using Li and ³⁵SO₄ to determine extracellular space (e.c.s.).

2. Resting concentrations after 1-2 hr incubation at 25° C in normal Krebs solution were: [Na]_i, 19·8 ± 0·9 m-mole (kg cell water)^-1; [K]_i, 192·7 ± 2·8 m-mole (kg cell water)^-1 (mean ± S.E. of mean of thirty-five ganglia). Correction for losses during e.c.s. measurement gave 22 mM [Na]_i and 207 mM [K]_i as probable fresh concentrations.

3. Carbachol (180 µM for 4 min) increased [Na]_i by 47·8 ± 2·9 m-mole (kg cell water)^-1 and decreased [K]_i by 54·6 ± 4·3 m-mole (kg cell water)^-1. Maximal exchange with carbachol or nicotine (at 1 mM for 4 min) amounted to 80-100 m-mole (kg cell water)^-1. On washing with Krebs solution containing 2·5 mM hexamethonium recovery of ionic concentrations occurred with a rate constant of 0·3-0·4 min^-1.

4. Restitution of ganglionic Na and K after carbachol was inhibited by washing with K-free solution, and slowed by ouabain (0·14 mM), cyanide (2 mM) or cooling (Q₁₀ 2·7 between 17 and 27° C).

5. Equilibrium potentials for Na and K (E_Na, E_K) at rest were calculated to be +49 and -88 mV. At a membrane potential (E_m) of -70 mV, the permeability ratio P_Na:P_K was calculated at 0·04:1 (assuming P_Cl:P_K < 0·1).

This article has been cited by other articles:

				E. O. Hernandez-Ochoa, R. E. Garcia-Ferreiro, and D. E. Garcia G protein activation inhibits gating charge movement in rat sympathetic neurons Am J Physiol Cell Physiol, June 1, 2007; 292(6): C2226 - C2238. [Abstract] [Full Text] [PDF]

				C. P. Taylor, M. L. Weber, C. L. Gaughan, E. J. Lehning, and R. M. LoPachin Oxygen/Glucose Deprivation in Hippocampal Slices: Altered Intraneuronal Elemental Composition Predicts Structural and Functional Damage J. Neurosci., January 15, 1999; 19(2): 619 - 629. [Abstract] [Full Text] [PDF]