J Physiol Boston Smyposia
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J Physiol Vol 243, Issue 1 pp 225-242
Copyright © 1974 by The Physiological Society
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Protein and glucose-induced changes in sodium transport across the pig small intestine

 C. Henriques de Jesus and M. W. Smith

1. The effect of glucose and of bovine {gamma}-globulin on short-circuit current and sodium transport was measured using new-born pig ileum before and after suckling.

2. Glucose increased the short-circuit current with an apparent Km of 1·9 and 19·5 mM for new-born unsuckled and suckled intestines respectively. The Vmax was about 80 µA cm-2 in each case.

3. Bovine {gamma}-globulin applied to new-born pig ileum caused a 20% increase in sodium chloride influx without change of short-circuit current. The tissue conductance was also greater in the presence of globulin. These effects were not seen in ileum taken from suckled pigs.

4. p-Chloromercuriphenyl sulphonic acid (PCMPS) inhibited the globulin induced increase in sodium influx in new-born pig ileum without changing the measured short-circuit current. 0·1 mM-PCMPS also changed the characteristics of the glucose-dependent increase in short-circuit current, so that this response came to resemble that seen in ileum taken from suckled pigs. Dithioerythritol reversed this effect of PCMPS.

5. The suckling-dependent difference in results is explained in terms of a change in microvillar function taking place as a consequence of long-term pinocytosis in vivo. The ability of PCMPS to simulate some of these changes suggests that sulphydryl groups might be important regulators of microvillar membrane stability.




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J. Pacha
Development of Intestinal Transport Function in Mammals
Physiol Rev, October 1, 2000; 80(4): 1633 - 1667.
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