| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1. Cyclic AMP was introduced into ventricular muscle by a cut-end method. Trabecular bundles were pulled through a partition which divided the preparation into a loading region and a test region. The loading region was exposed to Ca-free solution, cut transversely near the partition, and then briefly exposed to cyclic AMP. The test region was continually superfused with Tyrode soltuion. 2. In preliminary experiments, cell-to-cell movements were studied in long bundles by including [3H]cyclic AMP in the loading procedure and allowing redistribution to occur. After suitable test periods, the bundles were removed, frozen, and sliced into segments. Segment radioactivity was plotted against distance and fitted by a theoretical diffusion curve. 3. The results showed longitudinal redistribution of label over many cell lengths with an average effective diffusivity of 8 X 10(-7) cm2/sec. This value did not appear sensitive to the length of the test period or to the presence of a phosphodiesterase inhibitor. 4. The metabolic fate of cyclic AMP introduced by the cut-end method was determined by chromatographic separation of [3H]cyclic AMP and its break-down products. Most of the cyclic AMP was metabolized, but the results suggest that cell-to-cell movements of cyclic AMP contribute to the overall redistribution of label. 5. The cut-end method was used to study the influence of cyclic AMP on the contractile activity in the test region. Introduction of cyclic AMP evoked a delayed increase in twitch tension, about 25% above control. The inotropic effect peaked about 50 min after the end of the loading procedure, a delay which seemed compatible with slow longitudinal diffusion into the test region. 6. In control experiments, the cut-end procedure was repeated with 5'AMP (the immediate break-down product of cyclic AMP) instead of cyclic AMP. No delayed increase in twitch tension was observed. 7. Introduction of dibutyryl cyclic AMP increased twitch amplitude by 130%, with a delayed time course similar to that found for cyclic AMP. 8. The results using the cut-end procedure provide new evidence that cyclic AMP helps mediate adrenergic effects on the strength of contraction.
This article has been cited by other articles:
|
|
 |
|
  G. Kanaporis, G. Mese, L. Valiuniene, T. W. White, P. R. Brink, and V. Valiunas Gap Junction Channels Exhibit Connexin-specific Permeability to Cyclic Nucleotides J. Gen. Physiol., March 31, 2008; 131(4): 293 - 305. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V Valiunas, Y. Y Polosina, H Miller, I. A Potapova, L Valiuniene, S Doronin, R. T Mathias, R. B Robinson, M. R Rosen, I. S Cohen, et al. Connexin-specific cell-to-cell transfer of short interfering RNA by gap junctions J. Physiol., October 15, 2005; 568(2): 459 - 468. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. T. Nakanishi, T. C. Cope, M. M. Rich, D. I. Carrasco, and M. J. Pinter Regulation of Motoneuron Excitability via Motor Endplate Acetylcholine Receptor Activation J. Neurosci., March 2, 2005; 25(9): 2226 - 2232. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Swietach and R. D. Vaughan-Jones Novel method for measuring junctional proton permeation in isolated ventricular myocyte cell pairs Am J Physiol Heart Circ Physiol, November 1, 2004; 287(5): H2352 - H2363. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J E Klaunig, L M Kamendulis, and Y. Xu Epigenetic mechanisms of chemical carcinogenesis Human and Experimental Toxicology, October 1, 2000; 19(10): 543 - 555. [Abstract] [PDF]
|
|
|
|
 |
|
 C. Batias, J.-P. Siffroi, P. Fénichel, G. Pointis, and D. Segretain Connexin43 Gene Expression and Regulation in the Rodent Seminiferous Epithelium J. Histochem. Cytochem., June 1, 2000; 48(6): 793 - 806. [Abstract] [Full Text]
|
|
|
|
 |
|
 G. J. Christ and P. R. Brink Analysis of the Presence and Physiological Relevance of Subconducting States of Connexin43-Derived Gap Junction Channels in Cultured Human Corporal Vascular Smooth Muscle Cells Circ. Res., April 16, 1999; 84(7): 797 - 803. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. G. Bevans and A. L. Harris Direct High Affinity Modulation of Connexin Channel Activity by Cyclic Nucleotides J. Biol. Chem., February 5, 1999; 274(6): 3720 - 3725. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. I. Vaney, J. C. Nelson, and D. V. Pow Neurotransmitter Coupling through Gap Junctions in the Retina J. Neurosci., December 15, 1998; 18(24): 10594 - 10602. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. R. Rosen, I. S. Cohen, P. Danilo Jr., and S. F. Steinberg The heart remembers Cardiovasc Res, December 1, 1998; 40(3): 469 - 482. [Full Text] [PDF]
|
|
|
|
|
|
G. J. Christ, D. C. Spray, M. El-Sabban, L. K. Moore, and P. R. Brink Gap Junctions in Vascular Tissues: Evaluating the Role of Intercellular Communication in the Modulation of Vasomotor Tone Circ. Res., October 1, 1996; 79(4): 631 - 646. [Abstract] [Full Text]
|
|
|
|
|
|
R. D. Veenstra, H.-Z. Wang, D. A. Beblo, M. G. Chilton, A. L. Harris, E. C. Beyer, and P. R. Brink Selectivity of Connexin-Specific Gap Junctions Does Not Correlate With Channel Conductance Circ. Res., December 1, 1995; 77(6): 1156 - 1165. [Abstract] [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
