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1. Non-stimulated mast cells take up ⁸⁹Sr and ⁴⁵Ca. There is a rapid phase of uptake of both labels which is complete in 1 min and the ⁸⁹Sr uptake is similar in magnitude to the ⁴⁵Ca uptake. A further slower phase of uptake occurs in the period from 1 to 30 min of incubation. The magnitude of this slower phase is about 6 times greater for ⁸⁹Sr than for ⁴⁵Ca.

2. Non-stimulated and antigen-stimulated mast cells accumulate Sr which can be measured by atomic absorption spectroscopy. There is a direct relationship between Sr accumulation and histamine secretion which is independent of whether or not the cells are stimulated. 10% histamine secretion is associated with Sr accumulation of 0.25 f-mole/cell.

3. The time course of ⁸⁹Sr uptake is similar to the time course of histamine secretion in non-stimulated cells.

4. The uptake of ⁸⁹Sr is linearly related to the external Sr concentration in the range 0.5-16 m-mole/l. for both stimulated and non-stimulated cells.

5. Ca, 10-1000 µmole/l. inhibits ⁸⁹Sr uptake in non-stimulated cells.

6. Inhibition of glycolysis and oxidative phosphorylation prevents spontaneous histamine secretion in the presence of Sr without blocking the accumulation of Sr by the cells.

7. Dibutyryl cyclic AMP, 10 m-mole/l. produces only 18% inhibition of ⁸⁹Sr uptake whereas histamine secretion is inhibited by 45% by the same concentration. The antigen-stimulated Sr uptake on the other hand can be completely inhibited by dibutyryl cyclic AMP, 10 m-mole/l.

8. The uptakes of ⁸⁹Sr and Sr by unstimulated mast cells after 60 min incubation are dependent on extracellular H ion concentration. Both uptakes increase with increasing pH over the range pH 7-8.5.

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