HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Claret, M Articles by Giraud, F Search for Related Content PubMed PubMed Citation Articles by Claret, M Articles by Giraud, F

1. Human red blood cells (R.B.C.) were incubated with phosphatidylcholine vesicles to obtain partial depletion of their membrane cholesterol. The kinetic parameters of the Na pump (affinities and maximal fluxes) were determined in these cells and compared with controls. 2. In the presence of internal K, progressive cholesterol depletion induces gradually (i) an increase of the maximal flux (ii) a reduction of the apparent affinity for internal Na. 3. In the absence of internal K, cholesterol depletion has no apparent effect, indicating that the observed changes were mediated by the internal K. Cholesterol depletion acts by increasing these two K effects: (i) activation of the maximal fluxes, and (ii) reduction of the internal Na affinity. 4. The cholesterol effect (on the K-pump interaction) is specific; it occurs without any change (i) in the affinities for internal Na and inorganic phosphate and for external Na and K, and (ii) in the number of pumping sites. 5. Under physiological conditions, cholesterol reduces the cation translocation rate and increases the selectivity of the pump for internal Na.

This article has been cited by other articles:

				J. S. Bystriansky and J. S. Ballantyne Gill Na+-K+-ATPase activity correlates with basolateral membrane lipid composition in seawater- but not freshwater-acclimated Arctic char (Salvelinus alpinus) Am J Physiol Regulatory Integrative Comp Physiol, February 1, 2007; 292(2): R1043 - R1051. [Abstract] [Full Text] [PDF]

				A.C. Sposito Emerging insights into hypertension and dyslipidaemia synergies Eur. Heart J. Suppl., December 1, 2004; 6(suppl_G): G8 - G12. [Abstract] [Full Text] [PDF]

				J. ZICHA, C. D. NEGRIN, Z. DOBESOVA, F. CARR, M. VOKURKOVA, M. W. MCBRIDE, J. KUNES, and A. F. DOMINICZAK Altered Na+-K+ pump activity and plasma lipids in salt-hypertensive Dahl rats: relationship to Atp1a1 gene Physiol Genomics, July 17, 2001; 6(2): 99 - 104. [Abstract] [Full Text] [PDF]