HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Dawson, M J Articles by Wilkie, D R Search for Related Content PubMed PubMed Citation Articles by Dawson, M J Articles by Wilkie, D R

1. We have used phosphorus nuclear magnetic resonance (31P NMR) to study muscular fatigue in anaerobic amphibian muscle. In this paper the biochemical and energetic changes that result from a series of tetani are related to the decrease in rate constant (1/tau) for the final, exponential, phase of relaxation. 2. Using 31P NMR we have measured the concentrations of phosphocreatine (PCr), inorganic phosphate (Pi) and ATP as well as the internal pH. From our measurements we have calculated [creatine], [free ADP], the free-energy change (more precisely, the affinity A = -dG/d xi) for ATP hydrolysis and the rates of lactic acid production and of ATP hydrolysis. 3. We have found that 1/tau, the rate constant of relaxation, is correlated with each of the following, independently of the pattern of stimulation: isometric force production, all of the measured or calculated metabolite levels, pH and dG/d xi. 4. There is a clear dependence upon the pattern of stimulation of the relation between 1/tau and each of the following: total duration of the experiment, number of contractions, rate of lactic acid production and rate of ATP hydrolysis. 5. The rate of relaxation is linearly related to [PCr], [creatine], [Pi] and dG/d xi. It is nonlinearly related to isometric force, [ATP], [H+] and rate of ATP hydrolysis. 6. We conclude that the change in 1/tau, like that of isometric force, depends upon metabolic factors, and not upon any independent changes in the activation or deactivation of contraction. We suggest that 1/tau may depend upon the free-energy change for ATP hydrolysis which in turn may be related to the rate of Ca2+ uptake into the sarcoplasmic reticulum.

This article has been cited by other articles:

				D. G. Allen, G. D. Lamb, and H. Westerblad Skeletal Muscle Fatigue: Cellular Mechanisms Physiol Rev, January 1, 2008; 88(1): 287 - 332. [Abstract] [Full Text] [PDF]

				R. A. B. Lopes-Martins, R. L. Marcos, P. S. Leonardo, A. C. Prianti Jr., M. N. Muscara, F. Aimbire, L. Frigo, V. V. Iversen, and J. M. Bjordal Effect of low-level laser (Ga-Al-As 655 nm) on skeletal muscle fatigue induced by electrical stimulation in rats J Appl Physiol, July 1, 2006; 101(1): 283 - 288. [Abstract] [Full Text] [PDF]

				A. J. Cheng and C. L. Rice Fatigue and recovery of power and isometric torque following isotonic knee extensions J Appl Physiol, October 1, 2005; 99(4): 1446 - 1452. [Abstract] [Full Text] [PDF]

				C. R. Hancock, E. Janssen, and R. L. Terjung Skeletal muscle contractile performance and ADP accumulation in adenylate kinase-deficient mice Am J Physiol Cell Physiol, June 1, 2005; 288(6): C1287 - C1297. [Abstract] [Full Text] [PDF]

				C. R. Hancock, J. J. Brault, R. W. Wiseman, R. L. Terjung, and R. A. Meyer 31P-NMR observation of free ADP during fatiguing, repetitive contractions of murine skeletal muscle lacking AK1 Am J Physiol Cell Physiol, June 1, 2005; 288(6): C1298 - C1304. [Abstract] [Full Text] [PDF]

				C. A. Kindig, R. A. Howlett, C. M. Stary, B. Walsh, and M. C. Hogan Effects of acute creatine kinase inhibition on metabolism and tension development in isolated single myocytes J Appl Physiol, February 1, 2005; 98(2): 541 - 549. [Abstract] [Full Text] [PDF]

				A. M. Duke and D. S. Steele Mechanisms of reduced SR Ca2+ release induced by inorganic phosphate in rat skeletal muscle fibers Am J Physiol Cell Physiol, August 1, 2001; 281(2): C418 - C429. [Abstract] [Full Text] [PDF]

				J. H. Cieslar and G. P. Dobson Force reduction uncoupled from pH and H2PO4- in rat gastrocnemius in vivo with continuous 2-Hz stimulation Am J Physiol Regulatory Integrative Comp Physiol, August 1, 2001; 281(2): R511 - R518. [Abstract] [Full Text] [PDF]

				S. Hitchins, J. M. Cieslar, and G. P. Dobson 31P NMR quantitation of phosphorus metabolites in rat heart and skeletal muscle in vivo Am J Physiol Heart Circ Physiol, August 1, 2001; 281(2): H882 - H887. [Abstract] [Full Text] [PDF]

				R. A. Howlett, C. M. Stary, and M. C. Hogan Recovery of force during postcontractile depression in single Xenopus muscle fibers Am J Physiol Regulatory Integrative Comp Physiol, May 1, 2001; 280(5): R1469 - R1475. [Abstract] [Full Text] [PDF]

				A. J. DAHLSTEDT, A. KATZ, B. WIERINGA, and H. WESTERBLAD Is creatine kinase responsible for fatigue? Studies of isolated skeletal muscle deficient in creatine kinase FASEB J, May 1, 2000; 14(7): 982 - 990. [Abstract] [Full Text]

				J. D. Bruton, J. Lannergren, and H. Westerblad Effects of CO2-induced acidification on the fatigue resistance of single mouse muscle fibers at 28°C J Appl Physiol, August 1, 1998; 85(2): 478 - 483. [Abstract] [Full Text] [PDF]

				E. Van Lunteren, M. Moyer, and A. Torres ATP-sensitive K+ channel blocker glibenclamide and diaphragm fatigue during normoxia and hypoxia J Appl Physiol, August 1, 1998; 85(2): 601 - 608. [Abstract] [Full Text] [PDF]

				H. Tamaki, K. Kitada, T. Akamine, F. Murata, T. Sakou, and H. Kurata Alternate activity in the synergistic muscles during prolonged low-level contractions J Appl Physiol, June 1, 1998; 84(6): 1943 - 1951. [Abstract] [Full Text] [PDF]

				I. Hardewig, P. L. M. Van Dijk, and H. O. Portner High-energy turnover at low temperatures: recovery from exhaustive exercise in Antarctic and temperate eelpouts Am J Physiol Regulatory Integrative Comp Physiol, June 1, 1998; 274(6): R1789 - R1796. [Abstract] [Full Text] [PDF]

				N. K. Vollestad, I. Sejersted, and E. Saugen Mechanical behavior of skeletal muscle during intermittent voluntary isometric contractions in humans J Appl Physiol, November 1, 1997; 83(5): 1557 - 1565. [Abstract] [Full Text] [PDF]

				J. Booth, M. J. McKenna, P. A. Ruell, T. H. Gwinn, G. M. Davis, M. W. Thompson, A. R. Harmer, S. K. Hunter, and J. R. Sutton Impaired calcium pump function does not slow relaxation in human skeletal muscle after prolonged exercise J Appl Physiol, August 1, 1997; 83(2): 511 - 521. [Abstract] [Full Text] [PDF]

				E. Van Lunteren, A. Torres, and M. Moyer Effects of hypoxia on diaphragm relaxation rate during fatigue J Appl Physiol, May 1, 1997; 82(5): 1472 - 1478. [Abstract] [Full Text] [PDF]