HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Flatman, P W Search for Related Content PubMed PubMed Citation Articles by Flatman, P W

1. A method is described in which the concentration of ionized magnesium can be measured in intact red cells. The method uses an equilibrium dialysis technique originally developed by Ferreira & Lew (1976) and Flatman & Lew (1977) where the magnesium permeability of the red cell membrane is increased with the ionophore A23187. 2. The concentration of ionized magnesium in the oxygenated cells was found to be 0.39 mM and was not greatly affected by changes in the composition of the medium. 3. The concentration of ionized magnesium in deoxygenated cells showed more dependence on the composition of the medium. Values of 0.54 and 0.62 mM were found in cells incubated in Tris- and HCO3- buffered media respectively. The difference probably reflects increased competition between chloride and 2,3-diphosphoglycerate for common binding sites on haemoglobin in Tris-buffered cells. 3. Only a small increase of 0.16-0.22 mM was found in the concentration of ionized magnesium when the cells were deoxygenated. These changes are smaller than had been anticipated from estimates of the binding of ATP and 2,3-diphosphoglycerate to oxy- and deoxyhaemoglobin (Bunn, Ransil & Chao, 1971; Berger, Jänig, Gerber, Ruckpaul & Rapoport, 1973; Gerber, Berger, Jänig & Rapoport, 1973) and are unlikely to alter greatly the operation of magnesium-dependent metabolic or transport systems.

This article has been cited by other articles:

				P. W Flatman Activation of ferret erythrocyte Na+-K+-2Cl- cotransport by deoxygenation J. Physiol., March 1, 2005; 563(2): 421 - 431. [Abstract] [Full Text] [PDF]

				J. P. Willcocks, P. J. Mulquiney, J. C. Ellory, R. L. Veech, G. K. Radda, and K. Clarke Simultaneous Determination of Low Free Mg2+ and pH in Human Sickle Cells using 31P NMR Spectroscopy J. Biol. Chem., December 13, 2002; 277(51): 49911 - 49920. [Abstract] [Full Text] [PDF]

				M. Barbagallo, L. J. Dominguez, O. Bardicef, and L. M. Resnick Altered Cellular Magnesium Responsiveness to Hyperglycemia in Hypertensive Subjects Hypertension, September 1, 2001; 38(3): 612 - 615. [Abstract] [Full Text] [PDF]

				C. H. Joiner, M. Jiang, H. Fathallah, F. Giraud, and R. S. Franco Deoxygenation of sickle red blood cells stimulates KCl cotransport without affecting Na+/H+ exchange Am J Physiol Cell Physiol, June 1, 1998; 274(6): C1466 - C1475. [Abstract] [Full Text] [PDF]