| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
The major pathway of passive K influx (ouabain-insensitive) was characterized in low-K type (LK) red cells of sheep. 1. Passive K transport in these cells was highly sensitive to variations in cell volume; it increased threefold or more in cells swollen osmotically by 10%, and decreased up to twofold in cells shrunken 5-10%. Active K influx was insensitive to changes in cell volume. Three different methods for varying cell volume osmotically all gave similar results. 2. The volume-sensitive pathway was specific for K in that Na influx did not vary with changes in cell volume. 3. The volume-sensitive K influx was a saturable function of external K concentration. It was slightly inhibited by Na, whereas K influx in shrunken cells was unaffected by Na. 4. Passive K influx was dependent on the major anion in the medium in that replacement of Cl with any of six other anions resulted in a reduction of K influx by 50-80% (replacement of Cl by Br caused an increase in K influx). The activation of K influx by Cl followed sigmoid kinetics. 5. Passive K influx is inhibited by anti-L antibody. The antibody affected only that portion of influx which was Cl-dependent and volume-sensitve. Of the subfractions of the antibody, it is anti-L1 which inhibits passive K transport. 6. Pretreatment of cells with iodoacetamide reduced the sensitivity of K influx to cell volume in that the influx was reduced in swollen IAA-treated cells and increased in shrunken IAA-cells. 7. Intracellular Ca has no role in altering passive K transport in LK sheep cells. Therefore, the major pathway of passive K transport in LK sheep red cells is sensitive to changes in cell volume, specific for K, dependent on Cl, and inhibited by anti-L1 antibody, The minor pathway, observed in shrunken cells, has none of these properties.
This article has been cited by other articles:
|
|
 |
|
  J. A. Browning, H. M. Staines, H. C. Robinson, T. Powell, J. C. Ellory, and J. S. Gibson The effect of deoxygenation on whole-cell conductance of red blood cells from healthy individuals and patients with sickle cell disease Blood, March 15, 2007; 109(6): 2622 - 2629. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. B. E. Gagnon, R. England, and E. Delpire Characterization of SPAK and OSR1, Regulatory Kinases of the Na-K-2Cl Cotransporter Mol. Cell. Biol., January 15, 2006; 26(2): 689 - 698. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Mercado, V. Broumand, K. Zandi-Nejad, A. H. Enck, and D. B. Mount A C-terminal Domain in KCC2 Confers Constitutive K+-Cl- Cotransport J. Biol. Chem., January 13, 2006; 281(2): 1016 - 1026. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Mercado, N. Vazquez, L. Song, R. Cortes, A. H. Enck, R. Welch, E. Delpire, G. Gamba, and D. B. Mount NH2-terminal heterogeneity in the KCC3 K+-Cl- cotransporter Am J Physiol Renal Physiol, December 1, 2005; 289(6): F1246 - F1261. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Gamba Molecular Physiology and Pathophysiology of Electroneutral Cation-Chloride Cotransporters Physiol Rev, April 1, 2005; 85(2): 423 - 493. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. R. Williams and J. A. Payne Cation transport by the neuronal K+-Cl- cotransporter KCC2: thermodynamics and kinetics of alternate transport modes Am J Physiol Cell Physiol, October 1, 2004; 287(4): C919 - C931. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Fujise, K. Higa, T. Kanemaru, M. Fukuda, N. C. Adragna, and P. K. Lauf GSH depletion, K-Cl cotransport, and regulatory volume decrease in high-K/high-GSH dog red blood cells Am J Physiol Cell Physiol, December 1, 2001; 281(6): C2003 - C2009. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. L. Jennings and M. F. Adame Direct estimate of 1:1 stoichiometry of K+-Cl{-} cotransport in rabbit erythrocytes Am J Physiol Cell Physiol, September 1, 2001; 281(3): C825 - C832. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. S. GIBSON, A. KHAN, P. F. SPEAKE, and J. C. ELLORY O2 dependence of K+ transport in sickle cells: the effect of different cell populations and the substituted benzaldehyde 12C79 FASEB J, March 1, 2001; 15(3): 823 - 832. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. C. Muzyamba, P. F. Speake, and J. S. Gibson Oxidants and regulation of K+-Cl- cotransport in equine red blood cells Am J Physiol Cell Physiol, October 1, 2000; 279(4): C981 - C989. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. E. Race, F. N. Makhlouf, P. J. Logue, F. H. Wilson, P. B. Dunham, and E. J. Holtzman Molecular cloning and functional characterization of KCC3, a new K-Cl cotransporter Am J Physiol Cell Physiol, December 1, 1999; 277(6): C1210 - C1219. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Su, B. E. Shmukler, M. N. Chernova, A. K. Stuart-Tilley, L. de Franceschi, C. Brugnara, and S. L. Alper Mouse K-Cl cotransporter KCC1: cloning, mapping, pathological expression, and functional regulation Am J Physiol Cell Physiol, November 1, 1999; 277(5): C899 - C912. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. O. Bevensee, E. Bashi, W.-R. Schlue, G. Boyarsky, and W. F. Boron Shrinkage-induced activation of Na+/H+ exchange in rat renal mesangial cells Am J Physiol Cell Physiol, March 1, 1999; 276(3): C674 - C683. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. M. Gillen, S. Brill, J. A. Payne, and B. Forbush III Molecular Cloning and Functional Expression of the K-Cl Cotransporter from Rabbit, Rat, and Human. A NEW MEMBER OF THE CATION-CHLORIDE COTRANSPORTER FAMILY J. Biol. Chem., July 5, 1996; 271(27): 16237 - 16244. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
