HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Chesnut, T J Search for Related Content PubMed PubMed Citation Articles by Chesnut, T J

Desensitization to ionophoretically applied ACh has been studied at voltage-clamped neuromuscular junctions of Rana pipiens. The time courses of both the onset of and recovery from desensitization displayed two components. The application of metabolic inhibitors to the preparation decreased the degree of recovery and also decreased both time constants of onset. The effect on onset was more pronounced on the slow component while no effect selective for either of the two components of recovery was seen. Intracellular injection of EGTA into the muscle cell increased both time constants of desensitization onset. The effect was much more pronounced on the slow component. Increasing the dose of ACh selectively decreased the fast time constant of desensitization onset. The effect was more selective in low-calcium than normal Ringer solution. These observations suggest that there are at least two independent mechanisms in the process of desensitization at the neuromuscular junction. These mechanisms differ in time course, degree of dependency on [Ca]i, and sensitivity to acetylcholine dose.

This article has been cited by other articles:

				L. Khiroug, E. Sokolova, R. Giniatullin, R. Afzalov, and A. Nistri Recovery from Desensitization of Neuronal Nicotinic Acetylcholine Receptors of Rat Chromaffin Cells Is Modulated by Intracellular Calcium through Distinct Second Messengers J. Neurosci., April 1, 1998; 18(7): 2458 - 2466. [Abstract] [Full Text] [PDF]

				G. G. Wilson and A. Karlin Inaugural Article: Acetylcholine receptor channel structure in the resting, open, and desensitized states probed with the substituted-cysteine-accessibility method PNAS, January 30, 2001; 98(3): 1241 - 1248. [Abstract] [Full Text] [PDF]