HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Hodson, S Articles by Wigham, C Search for Related Content PubMed PubMed Citation Articles by Hodson, S Articles by Wigham, C

The fluxes of sodium, chloride and bicarbonate across endothelium plus stroma and then stroma alone were measured in the direction from lens-side to tear-side in rabbit and human corneas in vitro, in order to measure passive permeabilities. The results were used to calculate the permeability of the endothelium. Hodgkin's equation (1951) was then used to calculate the partial electrical conductivity of each ion crossing the endothelium. The summated electrical conductivities of sodium, chloride and bicarbonate were equal to 89 +/- 8% of the measured electrical conductivity, suggesting that the ions diffuse independently across the endothelium in the direction lens-side to tear-side. Stereological analysis of the intercellular spaces supports the idea that the ions permeate through this route and that the physical shape of the spaces determines almost entirely the permeability of the endothelial layer. Trans-endothelial sodium and chloride permeabilities are nearly equal, which may be explained by supposing the intercellular spaces include a cation exchanger of fixed negative charge capacity around 60 m-equiv l.-1 intercellular fluid.

This article has been cited by other articles:

				T. Mimura, S. Yokoo, M. Araie, S. Amano, and S. Yamagami Treatment of Rabbit Bullous Keratopathy with Precursors Derived from Cultured Human Corneal Endothelium Invest. Ophthalmol. Vis. Sci., October 1, 2005; 46(10): 3637 - 3644. [Abstract] [Full Text] [PDF]

				T. Mimura, S. Yamagami, S. Yokoo, Y. Yanagi, T. Usui, K. Ono, M. Araie, and S. Amano Sphere Therapy for Corneal Endothelium Deficiency in a Rabbit Model Invest. Ophthalmol. Vis. Sci., September 1, 2005; 46(9): 3128 - 3135. [Abstract] [Full Text] [PDF]

				S. Yokoo, S. Yamagami, Y. Yanagi, S. Uchida, T. Mimura, T. Usui, and S. Amano Human Corneal Endothelial Cell Precursors Isolated by Sphere-Forming Assay Invest. Ophthalmol. Vis. Sci., May 1, 2005; 46(5): 1626 - 1631. [Abstract] [Full Text] [PDF]

				T. Mimura, S. Yamagami, S. Yokoo, T. Usui, K. Tanaka, S. Hattori, S. Irie, K. Miyata, M. Araie, and S. Amano Cultured Human Corneal Endothelial Cell Transplantation with a Collagen Sheet in a Rabbit Model Invest. Ophthalmol. Vis. Sci., September 1, 2004; 45(9): 2992 - 2997. [Abstract] [Full Text] [PDF]