HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Cole, A E Articles by Nicoll, R A Search for Related Content PubMed PubMed Citation Articles by Cole, A E Articles by Nicoll, R A

Intracellular recording from CA1 pyramidal cells in the hippocampal slice preparation was used to compare the action of exogenously applied acetylcholine (ACh) and cholinomimetics to the effect of electrically stimulating sites in the slice known to contain cholinergic fibres. ACh depolarized pyramidal cells with an associated increase in input resistance, blocked a calcium-activated potassium conductance (GK(Ca], and blocked accommodation of action potential discharge. All of these actions were blocked by the muscarinic antagonist, atropine. Repetitive electrical stimulation of stratum (s.) oriens evoked a series of fast excitatory post-synaptic potentials (e.p.s.p.s) followed by an inhibitory post-synaptic potential. These potentials were followed by a slow e.p.s.p. that lasted 20-30 s. The slow e.p.s.p. was selectively enhanced by eserine and blocked by atropine. Ionophoretic application of ACh closely mimicked the time course of the slow e.p.s.p. The slow e.p.s.p. was blocked by tetrodotoxin and cadmium, indicating that it was dependent on propagated action potentials and on calcium. Considerably higher stimulus strengths were needed to elicit a slow e.p.s.p. than to elicit the earlier synaptic potentials. The size of the slow e.p.s.p. was markedly increased by repetitive stimulation. Stimulation of the alveus, s. oriens, s. pyramidale and fimbria all evoked a slow e.p.s.p., while stimulation of s. radiatum was relatively ineffective. The input resistance of the cell increased during the slow e.p.s.p. Hyperpolarizing the cell decreased the size of the slow e.p.s.p. and at membrane potentials of -70 mV or greater, little response was recorded. Stimulation of s. oriens blocked GK(Ca) and accommodation of action potential discharge. These effects, which could be seen in the absence of any change in membrane potential, were enhanced by eserine and blocked by atropine. The present electrophysiological results establish that CA1 pyramidal cells receive a cholinergic input and demonstrate that this input can dramatically alter the firing properties of these neurones for tens of seconds in the absence of any marked effect on membrane potential. Such an action contrasts with previously characterized synaptic potentials in this region of the brain.

This article has been cited by other articles:

				P. H. M. Kullmann and J. P. Horn Excitatory Muscarinic Modulation Strengthens Virtual Nicotinic Synapses on Sympathetic Neurons and Thereby Enhances Synaptic Gain J Neurophysiol, December 1, 2006; 96(6): 3104 - 3113. [Abstract] [Full Text] [PDF]

				S. Watanabe, M. Hong, N. Lasser-Ross, and W. N. Ross Modulation of calcium wave propagation in the dendrites and to the soma of rat hippocampal pyramidal neurons J. Physiol., September 1, 2006; 575(2): 455 - 468. [Abstract] [Full Text] [PDF]

				T. G.J. Allen, F. C. Abogadie, and D. A. Brown Simultaneous Release of Glutamate and Acetylcholine from Single Magnocellular "Cholinergic" Basal Forebrain Neurons J. Neurosci., February 1, 2006; 26(5): 1588 - 1595. [Abstract] [Full Text] [PDF]

				J. J. Lawrence, J. M. Statland, Z. M. Grinspan, and C. J. McBain Cell type-specific dependence of muscarinic signalling in mouse hippocampal stratum oriens interneurones J. Physiol., February 1, 2006; 570(3): 595 - 610. [Abstract] [Full Text] [PDF]

				T. Shinoe, M. Matsui, M. M. Taketo, and T. Manabe Modulation of Synaptic Plasticity by Physiological Activation of M1 Muscarinic Acetylcholine Receptors in the Mouse Hippocampus J. Neurosci., November 30, 2005; 25(48): 11194 - 11200. [Abstract] [Full Text] [PDF]

				P. K. Harrison, R. D. Sheridan, A. C. Green, I. R. Scott, and J. E. H. Tattersall A Guinea Pig Hippocampal Slice Model of Organophosphate-Induced Seizure Activity J. Pharmacol. Exp. Ther., August 1, 2004; 310(2): 678 - 686. [Abstract] [Full Text] [PDF]

				H. Romo-Parra, C. Vivar, J. Maqueda, M. A. Morales, and R. Gutierrez Activity-Dependent Induction of Multitransmitter Signaling Onto Pyramidal Cells and Interneurons of Hippocampal Area CA3 J Neurophysiol, June 1, 2003; 89(6): 3155 - 3167. [Abstract] [Full Text] [PDF]

				J. Kim, M. Isokawa, C. Ledent, and B. E. Alger Activation of Muscarinic Acetylcholine Receptors Enhances the Release of Endogenous Cannabinoids in the Hippocampus J. Neurosci., December 1, 2002; 22(23): 10182 - 10191. [Abstract] [Full Text] [PDF]

				M. H. Shalinsky, J. Magistretti, L. Ma, and A. A. Alonso Muscarinic Activation of a Cation Current and Associated Current Noise in Entorhinal-Cortex Layer-II Neurons J Neurophysiol, September 1, 2002; 88(3): 1197 - 1211. [Abstract] [Full Text] [PDF]

				M.-K. Sun and D. L. Alkon Impairment of Hippocampal CA1 Heterosynaptic Transformation and Spatial Memory by beta -Amyloid25-35 J Neurophysiol, May 1, 2002; 87(5): 2441 - 2449. [Abstract] [Full Text] [PDF]

				J. M. Power and P. Sah Nuclear Calcium Signaling Evoked by Cholinergic Stimulation in Hippocampal CA1 Pyramidal Neurons J. Neurosci., May 1, 2002; 22(9): 3454 - 3462. [Abstract] [Full Text] [PDF]

				E. S. L. Faber and P. Sah Physiological Role of Calcium-Activated Potassium Currents in the Rat Lateral Amygdala J. Neurosci., March 1, 2002; 22(5): 1618 - 1628. [Abstract] [Full Text] [PDF]

				X.-J. Wang Pacemaker Neurons for the Theta Rhythm and Their Synchronization in the Septohippocampal Reciprocal Loop J Neurophysiol, February 1, 2002; 87(2): 889 - 900. [Abstract] [Full Text] [PDF]

				M. E. Hasselmo and B. P. Fehlau Differences in Time Course of ACh and GABA Modulation of Excitatory Synaptic Potentials in Slices of Rat Hippocampus J Neurophysiol, October 1, 2001; 86(4): 1792 - 1802. [Abstract] [Full Text] [PDF]

				M.-K. Sun and D. L. Alkon Pharmacological Enhancement of Synaptic Efficacy, Spatial Learning, and Memory through Carbonic Anhydrase Activation in Rats J. Pharmacol. Exp. Ther., June 1, 2001; 297(3): 961 - 967. [Abstract] [Full Text]

				M.-K. Sun, D. Dahl, and D. L. Alkon Heterosynaptic Transformation of GABAergic Gating in the Hippocampus and Effects of Carbonic Anhydrase Inhibition J. Pharmacol. Exp. Ther., March 1, 2001; 296(3): 811 - 817. [Abstract] [Full Text]

				C. D. Fiorillo and J. T. Williams Cholinergic Inhibition of Ventral Midbrain Dopamine Neurons J. Neurosci., October 15, 2000; 20(20): 7855 - 7860. [Abstract] [Full Text] [PDF]

				H. Schobesberger, D. W. Wheeler, and J. P. Horn A Model for Pleiotropic Muscarinic Potentiation of Fast Synaptic Transmission J Neurophysiol, April 1, 2000; 83(4): 1912 - 1923. [Abstract] [Full Text] [PDF]

				P. E. Latham, B. J. Richmond, P. G. Nelson, and S. Nirenberg Intrinsic Dynamics in Neuronal Networks. I. Theory J Neurophysiol, February 1, 2000; 83(2): 808 - 827. [Abstract] [Full Text] [PDF]

				C. Weiss, A. R. Preston, M. M. Oh, R. D. Schwarz, D. Welty, and J. F. Disterhoft The M1 Muscarinic Agonist CI-1017 Facilitates Trace Eyeblink Conditioning in Aging Rabbits and Increases the Excitability of CA1 Pyramidal Neurons J. Neurosci., January 15, 2000; 20(2): 783 - 790. [Abstract] [Full Text] [PDF]

				M. M. Oh, J. M. Power, L. T. Thompson, P. L. Moriearty, and J. F. Disterhoft Metrifonate Increases Neuronal Excitability in CA1 Pyramidal Neurons from Both Young and Aging Rabbit Hippocampus J. Neurosci., March 1, 1999; 19(5): 1814 - 1823. [Abstract] [Full Text] [PDF]

				M. J. Marino, S. T. Rouse, A. I. Levey, L. T. Potter, and P. J. Conn Activation of the genetically defined m1 muscarinic receptor potentiates N-methyl-D-aspartate (NMDA) receptor currents in hippocampal pyramidal cells PNAS, September 15, 1998; 95(19): 11465 - 11470. [Abstract] [Full Text] [PDF]

				L. A. Zimmer, M. Ennis, R. G. Wiley, and M. T. Shipley Nerve Gas-Induced Seizures: Role of Acetylcholine in the Rapid Induction of Fos and Glial Fibrillary Acidic Protein in Piriform Cortex J. Neurosci., May 15, 1998; 18(10): 3897 - 3908. [Abstract] [Full Text] [PDF]

				M. F. Schmidt and D. J. Perkel Slow Synaptic Inhibition in Nucleus HVc of the Adult Zebra Finch J. Neurosci., February 1, 1998; 18(3): 895 - 904. [Abstract] [Full Text] [PDF]

				Y. Zhang, P. L. Carlen, and L. Zhang Kinetics of Muscarinic Reduction of IsAHP in Hippocampal Neurons: Effects of Acetylcholinesterase Inhibitors J Neurophysiol, December 1, 1997; 78(6): 2999 - 3007. [Abstract] [Full Text] [PDF]

				C. A. Chapman and R. J. Racine Converging Inputs to the Entorhinal Cortex From the Piriform Cortex and Medial Septum: Facilitation and Current Source Density Analysis J Neurophysiol, November 1, 1997; 78(5): 2602 - 2615. [Abstract] [Full Text] [PDF]

				K. Kaila, K. Lamsa, S. Smirnov, T. Taira, and J. Voipio Long-Lasting GABA-Mediated Depolarization Evoked by High-Frequency Stimulation in Pyramidal Neurons of Rat Hippocampal Slice Is Attributable to a Network-Driven, Bicarbonate-Dependent K+ Transient J. Neurosci., October 15, 1997; 17(20): 7662 - 7672. [Abstract] [Full Text] [PDF]

				H. Tsubokawa and W. N. Ross Muscarinic Modulation of Spike Backpropagation in the Apical Dendrites of Hippocampal CA1 Pyramidal Neurons J. Neurosci., August 1, 1997; 17(15): 5782 - 5791. [Abstract] [Full Text] [PDF]

				F. Gonon Prolonged and Extrasynaptic Excitatory Action of Dopamine Mediated by D1 Receptors in the Rat Striatum In Vivo J. Neurosci., August 1, 1997; 17(15): 5972 - 5978. [Abstract] [Full Text] [PDF]

				P. Congar, X. Leinekugel, Y. Ben-Ari, and V. Crepel A Long-Lasting Calcium-Activated Nonselective Cationic Current Is Generated by Synaptic Stimulation or Exogenous Activation of Group I Metabotropic Glutamate Receptors in CA1 Pyramidal Neurons J. Neurosci., July 15, 1997; 17(14): 5366 - 5379. [Abstract] [Full Text] [PDF]

				R. Klink and A. Alonso Muscarinic Modulation of the Oscillatory and Repetitive Firing Properties of Entorhinal Cortex Layer II Neurons J Neurophysiol, April 1, 1997; 77(4): 1813 - 1828. [Abstract] [Full Text] [PDF]

				J. R. Moyer Jr., L. T. Thompson, and J. F. Disterhoft Trace Eyeblink Conditioning Increases CA1 Excitability in a Transient and Learning-Specific Manner J. Neurosci., September 1, 1996; 16(17): 5536 - 5546. [Abstract] [Full Text] [PDF]

				M.E. Hasselmo, M.A. Wilson, B.R. Anderson, and J.M. Bower Associative Memory Function in Piriform (Olfactory) Cortex: Computational Modeling and Neuropharmacology Cold Spring Harb Symp Quant Biol, January 1, 1990; 55(0): 599 - 610. [Abstract] [PDF]

				G. O'Meara, U. Coumis, S. Y. Ma, J. Kehr, S. Mahoney, A. Bacon, S. J. Allen, F. Holmes, U. Kahl, F. H. Wang, et al. Galanin regulates the postnatal survival of a subset of basal forebrain cholinergic neurons PNAS, October 10, 2000; 97(21): 11569 - 11574. [Abstract] [Full Text] [PDF]