| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
The electrophysiological properties of the cell membrane of rat spermatogenic cells were studied using the whole-cell variation of the patch-electrode voltage-clamp technique. In late primary spermatocytes and early spermatids isolated from adult testis, a transient inward current followed by a slowly developing outward current was produced when the membrane potential was made more positive than -60 mV. Early spermatogenic cells which consist of spermatogonia and early spermatocytes were isolated either from new-born rats (12-14 days old) of from adult cryptorchid rats 15-21 days after the operation. In early spermatogenic cells, some showed a slowly developing outward current with negligible initial inward current, while others showed a recognizable inward current followed by the slowly developing outward current. The inward currents are identified as Ca2+-carried current, since replacement of external Ca2+ with Mn2+ reversibly diminished the current whereas Ba2+ or Sr2+ substituted for Ca2+. The reversal potential of the outward current changed from -65 to -12 mV when [K+]o was raised from 5 to 100 mM. The outward current was independent of [Ca2+]o and was blocked by tetraethylammonium chloride. Thus the current was identified as membrane-potential-dependent K+ current. During spermatogenesis from spermatogonia to early spermatids, the density of Ca2+ current increased while the K+ current density decreased significantly.
This article has been cited by other articles:
|
|
 |
|
  C. Jimenez-Gonzalez, F. Michelangeli, C.V. Harper, C.L.R. Barratt, and S.J. Publicover Calcium signalling in human spermatozoa: a specialized 'toolkit' of channels, transporters and stores Hum. Reprod. Update, May 1, 2006; 12(3): 253 - 267. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Di Zhang and M. Gopalakrishnan Sperm Ion Channels: Molecular Targets for the Next Generation of Contraceptive Medicines? J Androl, November 1, 2005; 26(6): 643 - 653. [Full Text] [PDF]
|
|
|
|
 |
|
 K Wagoner, G Sanchez, A-N Nguyen, G C Enders, and G Blanco Different expression and activity of the {alpha}1 and {alpha}4 isoforms of the Na,K-ATPase during rat male germ cell ontogeny Reproduction, November 1, 2005; 130(5): 627 - 641. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Ben-Aharon, P. R Brown, N. Etkovitz, E. M Eddy, and R. Shalgi The expression of calpain 1 and calpain 2 in spermatogenic cells and spermatozoa of the mouse Reproduction, April 1, 2005; 129(4): 435 - 442. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Tosti and R. Boni Electrical events during gamete maturation and fertilization in animals and humans Hum. Reprod. Update, January 1, 2004; 10(1): 53 - 65. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Jagannathan, E. L. Punt, Y. Gu, C. Arnoult, D. Sakkas, C. L. R. Barratt, and S. J. Publicover Identification and Localization of T-type Voltage-operated Calcium Channel Subunits in Human Male Germ Cells. EXPRESSION OF MULTIPLE ISOFORMS J. Biol. Chem., March 1, 2002; 277(10): 8449 - 8456. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X.D. Gong, J.C.H. Li, K.H. Cheung, G.P.H. Leung, S.B.C. Chew, and P.Y.D. Wong Expression of the cystic fibrosis transmembrane conductance regulator in rat spermatids: implication for the site of action of antispermatogenic agents Mol. Hum. Reprod., August 1, 2001; 7(8): 705 - 713. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Rossato, F.D. Virgilio, R. Rizzuto, C. Galeazzi, and C. Foresta Intracellular calcium store depletion and acrosome reaction in human spermatozoa: role of calcium and plasma membrane potential Mol. Hum. Reprod., February 1, 2001; 7(2): 119 - 128. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. M.B. O'Toole, C. Arnoult, A. Darszon, R. A. Steinhardt, and H. M. Florman Ca2+ Entry through Store-operated Channels in Mouse Sperm Is Initiated by Egg ZP3 and Drives the Acrosome Reaction Mol. Biol. Cell, May 1, 2000; 11(5): 1571 - 1584. [Abstract] [Full Text]
|
|
|
|
 |
|
 C. Arnoult, I. G. Kazam, P. E. Visconti, G. S. Kopf, M. Villaz, and H. M. Florman Control of the low voltage-activated calcium channel of mouse sperm by egg ZP3 and by membrane hyperpolarization during capacitation PNAS, June 8, 1999; 96(12): 6757 - 6762. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S.J. Publicover and C.L.R. Barratt Voltage-operated Ca2+ channels and the acrosome reaction: which channels are present and what do they do? Hum. Reprod., April 1, 1999; 14(4): 873 - 879. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Darszon, P. Labarca, T. Nishigaki, and F. Espinosa Ion Channels in Sperm Physiology Physiol Rev, April 1, 1999; 79(2): 481 - 510. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. M. Florman, C. Arnoult, I. G. Kazam, C. Li, and C. M.B. O'Toole A Perspective on the Control of Mammalian Fertilization by Egg-Activated Ion Channels in Sperm: A Tale of Two Channels Biol Reprod, July 1, 1998; 59(1): 12 - 16. [Full Text]
|
|
|
|
 |
|
 C. Arnoult, M. Villaz, and H. M. Florman Pharmacological Properties of the T-Type Ca2+ Current of Mouse Spermatogenic Cells Mol. Pharmacol., June 1, 1998; 53(6): 1104 - 1111. [Abstract] [Full Text]
|
|
|
|
|
|
C. Arnoult, R. A. Cardullo, J. R. Lemos, and H. M. Florman Activation of mouse sperm T-type Ca2+ channels by adhesion to the egg zona pellucida PNAS, November 12, 1996; 93(23): 13004 - 13009. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Wennemuth, R. E. Westenbroek, T. Xu, B. Hille, and D. F. Babcock CaV2.2 and CaV2.3 (N- and R-type) Ca2+ Channels in Depolarization-evoked Entry of Ca2+ into Mouse Sperm J. Biol. Chem., July 7, 2000; 275(28): 21210 - 21217. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
