J Physiol Society Membership
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Physiol Vol 357 pp 173-183
Copyright © 1984 by The Physiological Society
This Article
Right arrow Full Text (PDF)
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Miledi, R
Right arrow Articles by Parker, I
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Miledi, R
Right arrow Articles by Parker, I

Chloride current induced by injection of calcium into Xenopus oocytes.

 R Miledi and I Parker

Membrane currents of Xenopus oocytes were studied with the membrane under voltage clamp. Intracellular injection of the calcium-chelating agent EGTA reduced, or abolished, the transient outward chloride current normally activated by membrane depolarization. Intracellular injection of calcium ions evoked large membrane currents, which inverted direction close to the chloride equilibrium potential. Injections of strontium, or barium, were less effective than calcium, while magnesium was ineffective. Large chloride currents could be evoked by calcium injections in oocytes which showed only small or no transient outward currents. The current activated by calcium injection increased with increasing depolarization up to high (ca. +60 mV) positive potentials, even though the transient outward current was suppressed by strong depolarization. The results indicate that the transient outward current depends upon entry of calcium through voltage-gated calcium ion channels and show that the oocyte membrane contains numerous chloride channels which are activated by intracellular calcium. Only a few of these chloride channels are activated by depolarization.




This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
T. Boulin, M. Gielen, J. E. Richmond, D. C. Williams, P. Paoletti, and J.-L. Bessereau
Eight genes are required for functional reconstitution of the Caenorhabditis elegans levamisole-sensitive acetylcholine receptor
PNAS, November 25, 2008; 105(47): 18590 - 18595.
[Abstract] [Full Text] [PDF]

Home page
 Anesth. Analg.Home page
C.-X. Yang, H. Xu, K.-Q. Zhou, M.-Y. Wang, and T.-L. Xu
Modulation of Gamma-Aminobutyric AcidA Receptor Function by Thiopental in the Rat Spinal Dorsal Horn Neurons.
Anesth. Analg., April 1, 2006; 102(4): 1114 - 1120.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
A. N. Placzek, F. Grassi, E. M Meyer, and R. L. Papke
An {alpha}7 Nicotinic Acetylcholine Receptor Gain-of-Function Mutant That Retains Pharmacological Fidelity
Mol. Pharmacol., December 1, 2005; 68(6): 1863 - 1876.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
E. Palma, G. Spinelli, G. Torchia, A. Martinez-Torres, D. Ragozzino, R. Miledi, and F. Eusebi
Abnormal GABAA receptors from the human epileptic hippocampal subiculum microtransplanted to Xenopus oocytes
PNAS, February 15, 2005; 102(7): 2514 - 2518.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
L. M. Solis-Garrido, A. J. Pintado, E. Andres-Mateos, M. Figueroa, C. Matute, and C. Montiel
Cross-talk between Native Plasmalemmal Na+/Ca2+ Exchanger and Inositol 1,4,5-Trisphosphate-sensitive Ca2+ Internal Store in Xenopus Oocytes
J. Biol. Chem., December 10, 2004; 279(50): 52414 - 52424.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
L. Liu, I. Markus, R. J. Vandenberg, B. A. Neilan, M. Murray, and E. Burcher
Molecular identification and characterization of three isoforms of tachykinin NK1-like receptors in the cane toad Bufo marinus
Am J Physiol Regulatory Integrative Comp Physiol, September 1, 2004; 287(3): R575 - R585.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
N. Strutz-Seebohm, M. Werner, D. M. Madsen, G. Seebohm, Y. Zheng, C. S. Walker, A. V. Maricq, and M. Hollmann
Functional Analysis of Caenorhabditis elegans Glutamate Receptor Subunits by Domain Transplantation
J. Biol. Chem., November 7, 2003; 278(45): 44691 - 44701.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
P. Roncaglia, P. Mistrik, and V. Torre
Pore Topology of the Hyperpolarization-Activated Cyclic Nucleotide-Gated Channel from Sea Urchin Sperm
Biophys. J., October 1, 2002; 83(4): 1953 - 1964.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
C. J. Herrero, E. Ales, A. J. Pintado, M. G. Lopez, E. Garcia-Palomero, S. K. Mahata, D. T. O'Connor, A. G. Garcia, and C. Montiel
Modulatory Mechanism of the Endogenous Peptide Catestatin on Neuronal Nicotinic Acetylcholine Receptors and Exocytosis
J. Neurosci., January 15, 2002; 22(2): 377 - 388.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
M. B. Gingrich, C. E. Junge, P. Lyuboslavsky, and S. F. Traynelis
Potentiation of NMDA Receptor Function by the Serine Protease Thrombin
J. Neurosci., June 15, 2000; 20(12): 4582 - 4595.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
N. Callamaras and I. Parker
Ca2+-dependent activation of Cl- currents in Xenopus oocytes is modulated by voltage
Am J Physiol Cell Physiol, April 1, 2000; 278(4): C667 - C675.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
A. P. Haghighi and E. Cooper
A Molecular Link between Inward Rectification and Calcium Permeability of Neuronal Nicotinic Acetylcholine alpha 3beta 4 and alpha 4beta 2 Receptors
J. Neurosci., January 15, 2000; 20(2): 529 - 541.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
A. Takahashi, P. Camacho, J. D. Lechleiter, and B. Herman
Measurement of Intracellular Calcium
Physiol Rev, October 1, 1999; 79(4): 1089 - 1125.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
S. M. Logan, F. E. Rivera, and J. P. Leonard
Protein Kinase C Modulation of Recombinant NMDA Receptor Currents: Roles for the C-Terminal C1 Exon and Calcium Ions
J. Neurosci., February 1, 1999; 19(3): 974 - 986.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
A. Kuruma and H. C. Hartzell
Dynamics of calcium regulation of chloride currents in Xenopus oocytes
Am J Physiol Cell Physiol, January 1, 1999; 276(1): C161 - C175.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
K. TAKAHASHI and Y. OKAMURA
Ion Channels and Early Development of Neural Cells
Physiol Rev, April 1, 1998; 78(2): 307 - 337.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
R. O. Arellano, E. Garay, and R. Miledi
Cl- currents activated via purinergic receptors in Xenopus follicles
Am J Physiol Cell Physiol, February 1, 1998; 274(2): C333 - C340.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
H.-L. Ji, M. D. Duvall, H. K. Patton, C. L. Satterfield, C. M. Fuller, and D. J. Benos
Functional expression of a truncated Ca2+-activated Cl- channel and activation by phorbol ester
Am J Physiol Cell Physiol, February 1, 1998; 274(2): C455 - C464.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
T. Nishizaki, T. Matsuoka, T. Nomura, K. Sumikawa, T. Shiotani, S. Watabe, and M. Yoshii
Nefiracetam Modulates Acetylcholine Receptor Currents via Two Different Signal Transduction Pathways
Mol. Pharmacol., January 1, 1998; 53(1): 1 - 5.
[Abstract] [Full Text]

Home page
 Proc. Natl. Acad. Sci. USAHome page
T. Blank, I. Nijholt, U. Teichert, H. Kugler, H. Behrsing, A. Fienberg, P. Greengard, and J. Spiess
The phosphoprotein DARPP-32 mediates cAMP-dependent potentiation of striatal N-methyl-D-aspartate responses
PNAS, December 23, 1997; 94(26): 14859 - 14864.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
I. A. Omelchenko, C. S. Nelson, and C. N. Allen
Lead Inhibition of N-Methyl-D-aspartate Receptors Containing NR2A, NR2C and NR2D Subunits
J. Pharmacol. Exp. Ther., September 1, 1997; 282(3): 1458 - 1464.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
S. DeLisle, E. W. Marksberry, C. Bonnett, D. J. Jenkins, B. V.L. Potter, M. Takahashi, and K. Tanzawa
Adenophostin A Can Stimulate Ca2+ Influx without Depleting the Inositol 1,4,5-Trisphosphate-sensitive Ca2+ Stores in the Xenopus Oocyte
J. Biol. Chem., April 11, 1997; 272(15): 9956 - 9961.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
Y. G. Ni, N. Camacho, and R. Miledi
Irreversible antagonism of 5HT2c receptors by N-ethoxycarbonyl-2-ethoxy-1,2-dihydroquinoline (EEDQ)
PNAS, March 18, 1997; 94(6): 2715 - 2718.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
Y. G. Ni and R. Miledi
Blockage of 5HT2C serotonin receptors by fluoxetine (Prozac)
PNAS, March 4, 1997; 94(5): 2036 - 2040.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
T. Matsuoka, T. Nishizaki, and K. Sumino
A Specific Inhibitory Action of Lithium on the 5-HT2c Receptor Expressed in Xenopus laevis Oocytes
Mol. Pharmacol., March 1, 1997; 51(3): 471 - 474.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
G. Buyse, T. Voets, J. Tytgat, C. De Greef, G. Droogmans, B. Nilius, and J. Eggermont
Expression of Human pICln and ClC-6 in Xenopus Oocytes Induces an Identical Endogenous Chloride Conductance
J. Biol. Chem., February 7, 1997; 272(6): 3615 - 3621.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
L. E. Chavez-Noriega, J. H. Crona, M. S. Washburn, A. Urrutia, K. J. Elliott, and E. C. Johnson
Pharmacological Characterization of Recombinant Human Neuronal Nicotinic Acetylcholine Receptors halpha 2beta 2, halpha 2beta 4, halpha 3beta 2, halpha 3beta 4, halpha 4beta 2, halpha 4beta 4 and halpha 7 Expressed in Xenopus Oocytes
J. Pharmacol. Exp. Ther., January 1, 1997; 280(1): 346 - 356.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
P. Gilon, G. St. J. Bird, X. Bian, J. L. Yakel, and J. W. Putney Jr.
The Ca[IMAGE]-mobilizing Actions of a Jurkat Cell Extract on Mammalian Cells and Xenopus laevis Oocytes
J. Biol. Chem., April 7, 1995; 270(14): 8050 - 8055.
[Abstract] [Full Text] [PDF]

Home page
 Cold Spring Harb Symp Quant BiolHome page
S. Numa, K. Fukuda, T. Kubo, A. Maeda, I. Akiba, H. Bujo, J. Nakai, M. Mishina, and H. Higashida
Molecular Basis of the Functional Heterogeneity of the Muscarinic Acetylcholine Receptor
Cold Spring Harb Symp Quant Biol, January 1, 1988; 53(0): 295 - 301.
[Abstract] [PDF]

Home page
 Cold Spring Harb Symp Quant BiolHome page
M.J. Berridge and C.W. Taylor
Inositol Trisphosphate and Calcium Signaling
Cold Spring Harb Symp Quant Biol, January 1, 1988; 53(0): 927 - 933.
[Abstract] [PDF]

Home page
 J. Biol. Chem.Home page
C. Garcia-Martinez, C. Morenilla-Palao, R. Planells-Cases, J. M. Merino, and A. Ferrer-Montiel
Identification of an Aspartic Residue in the P-loop of the Vanilloid Receptor That Modulates Pore Properties
J. Biol. Chem., October 13, 2000; 275(42): 32552 - 32558.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1984 The Physiological Society.