| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
The whole-cell patch-clamp method was used to study the voltage-gated K+ conductance of human peripheral blood T lymphocytes. After entry into whole-cell recording mode, there are time-dependent changes in some properties of the conductance. Over the first 10-30 min, the threshold for activation shifts about 10 mV more negative, and the rates of activation and inactivation increase. Inactivation is less strongly voltage dependent than activation or deactivation. Lymphocytes were stimulated to proliferate in culture with the tumour promoter 12-O-tetradecanoylphorbol-13-acetate (TPA). No changes in K+ conductance were observed in the first few hours of TPA stimulation. At 24 h after mitogen addition, TPA-treated cells were found to have 1.7-fold greater average voltage-gated K+ conductance than unstimulated control cells. At 48 h, TPA-stimulated cells had the same average K+ conductance as at 24 h, even though the cells were now much increased in size, as measured by cell capacitance. DNA synthesis by cultures stimulated with TPA, phytohaemagglutinin or succinyl concanavalin A was depressed by the addition of 0.1 mM-quinine at any point in the culture period. In the first 20 h after mitogen addition, DNA synthesis was more effectively inhibited by quinine than if the drug were added later. Cell proliferation was equally sensitive to quinine regardless of mitogen.
This article has been cited by other articles:
|
|
 |
|
  R. Vicente, A. Escalada, C. Soler, M. Grande, A. Celada, M. M. Tamkun, C. Solsona, and A. Felipe Pattern of Kv{beta} Subunit Expression in Macrophages Depends upon Proliferation and the Mode of Activation J. Immunol., April 15, 2005; 174(8): 4736 - 4744. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Bagdaany, C. V. F. Batista, N. A. Valdez-Cruz, S. Somodi, R. C. R. de la Vega, A. F. Licea, Z. Varga, R. Gaspar, L. D. Possani, and G. Panyi Anuroctoxin, a New Scorpion Toxin of the {alpha}-KTx 6 Subfamily, Is Highly Selective for Kv1.3 over IKCa1 Ion Channels of Human T Lymphocytes Mol. Pharmacol., April 1, 2005; 67(4): 1034 - 1044. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Q.-H. Liu, B. K. Fleischmann, B. Hondowicz, C. C. Maier, L. A. Turka, K. Yui, M. I. Kotlikoff, A. D. Wells, and B. D. Freedman Modulation of Kv Channel Expression and Function by TCR and Costimulatory Signals during Peripheral CD4+ Lymphocyte Differentiation J. Exp. Med., October 7, 2002; 196(7): 897 - 909. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. F. Fomina, C. M. Fanger, J. A. Kozak, and M. D. Cahalan Single Channel Properties and Regulated Expression of Ca2+ Release-Activated Ca2+ (CRAC) Channels in Human T Cells J. Cell Biol., September 18, 2000; 150(6): 1435 - 1444. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Xu, L. Wang, W. Dai, and L. Lu A Requirement for K+-Channel Activity in Growth Factor-Mediated Extracellular Signal-Regulated Kinase Activation in Human Myeloblastic Leukemia ML-1 Cells Blood, July 1, 1999; 94(1): 139 - 145. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Szabo, E. Gulbins, H. Apfel, X. Zhang, P. Barth, A. E. Busch, K. Schlottmann, O. Pongs, and F. Lang Tyrosine Phosphorylation-dependent Suppression of a Voltage-gated K+ Channel in T Lymphocytes upon Fas Stimulation J. Biol. Chem., August 23, 1996; 271(34): 20465 - 20469. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Ghanshani, H. Wulff, M. J. Miller, H. Rohm, A. Neben, G. A. Gutman, M. D. Cahalan, and K. G. Chandy Up-regulation of the IKCa1 Potassium Channel during T-cell Activation. MOLECULAR MECHANISM AND FUNCTIONAL CONSEQUENCES J. Biol. Chem., November 17, 2000; 275(47): 37137 - 37149. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
