HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Acevedo, M Articles by Ward, M R Search for Related Content PubMed PubMed Citation Articles by Acevedo, M Articles by Ward, M R

Department of Anatomy and Physiology, University of Dundee.

1. The ionic permeabilities of the apical and basolateral membranes of sheep tracheal epithelial cells have been evaluated by studying the effect of ion replacements on the apical (Va) and basolateral (Vb) membrane potentials and apical voltage divider ratio (fa). 2. The results provide evidence for the existence of conductances for Cl-, Na+ and K+ at the apical membrane, and of an important K+ conductance at the basolateral membrane. These characteristics agree with the model for Cl- secretion and Na+ absorption proposed for tracheal epithelium of other species. 3. The short-circuit current (Isc) across this tissue was found to increase significantly when HCO3- and CO2 were added to the bathing solutions. The addition of 0.5 mM-SITS (4-acetamido-4'-isothiocyanostilbene-2,2'-disulphonic acid) to the serosal bathing solution decreased Isc only if HCO3- and CO2 were present in the bathing solutions. These results suggest the existence of a HCO3- secretion in sheep tracheal epithelium under short-circuit conditions. 4. The results obtained in HCO3- replacement studies are compatible with an electrically silent HCO3- entry across the basolateral membrane and an electrogenic exit at the apical membrane of the cells.

This article has been cited by other articles:

				S. K. Inglis, L. Finlay, S. J. Ramminger, K. Richard, M. R. Ward, S. M. Wilson, and R. E. Olver Regulation of intracellular pH in Calu-3 human airway cells J. Physiol., January 9, 2002; 538(2): 527 - 539. [Abstract] [Full Text] [PDF]