HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Hirsch, J C Articles by Crepel, F Search for Related Content PubMed PubMed Citation Articles by Hirsch, J C Articles by Crepel, F

CNRS URA 1121, Université Paris-Sud, Orsay, France.

1. An in vitro slice preparation of rat prefrontal cortex was used to analyse the responses of layer V pyramidal cells to electrical stimulation of layer II. We also studied the long-lasting modifications of synaptic efficacy following high-frequency stimulation of the same region. 2. Stable intracellular recordings were obtained from forty-three regular spiking pyramidal cells. The input resistance was 56 +/- 18 M omega (mean +/- S.D.) at a resting membrane potential of -71 +/- 4 mV. 3. At rest, a single stimulus of increasing strength evoked a monophasic, purely depolarizing postsynaptic potential (PSP) of increasing amplitude. In neurons recorded with potassium acetate-filled micropipettes, membrane depolarization disclosed an excitatory-inhibitory (EPSP-IPSP) sequence (onset latency of the EPSP, 3.6 +/- 0.6 ms). 4. Superfusion with the non-N-methyl-D-aspartate (NMDA) receptor antagonist, 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX) reduced the EPSP and suppressed the IPSP. The small EPSP which remained was blocked by the NMDA receptor antagonist, D,L-2-amino-5-phosphonovalerate (APV). 5. In five cells, administration of 0.5 mumol l-1 bicuculline revealed a postsynaptic NMDA component in the evoked response as evidenced by its anomalous voltage dependence in the presence of Mg2+ and its sensitivity to APV. In these cells the latency of the APV-sensitive EPSP was the same as that of the APV-insensitive EPSP. 6. In six cells superfused with a high-Mg2+, low-Ca2+ artificial cerebrospinal fluid (ACSF) a small monosynaptic EPSP remained which had the same latency as the PSP recorded in control ACSF. 7. Patterned high-frequency stimulation (50-100 Hz) was applied to the afferents of twenty-eight neurons (twenty-three of them were recorded in the presence of bicuculline). During the train the membrane potential depolarized some 20 mV and each stimulus evoked a small PSP. The tetanic stimulation was followed by a short-term enhancement of the PSP amplitude and a slight increase in membrane input resistance. 8. Out of the twenty-eight cells, twenty-four showed long-lasting (over 30 min) modifications of the PSP. Long-term depression (LTD) of the evoked PSP was observed in fourteen cells and long-term potentiation (LTP) in ten cells. There was no significant change in the steady-state membrane properties and in the latency of the response. 9. In 64% of the cells that showed LTD and 70% of those that showed LTP of synaptic efficacy, the latency of the enhanced or depressed component of the PSP was the same as the control.(ABSTRACT TRUNCATED AT 400 WORDS)

This article has been cited by other articles:

				L. Chen, J. D. Bohanick, M. Nishihara, J. K. Seamans, and C. R. Yang Dopamine D1/5 Receptor-Mediated Long-Term Potentiation of Intrinsic Excitability in Rat Prefrontal Cortical Neurons: Ca2+-Dependent Intracellular Signaling J Neurophysiol, March 1, 2007; 97(3): 2448 - 2464. [Abstract] [Full Text] [PDF]

				E. H. Baeg, Y. B. Kim, J. Kim, J.-W. Ghim, J. J. Kim, and M. W. Jung Learning-Induced Enduring Changes in Functional Connectivity among Prefrontal Cortical Neurons J. Neurosci., January 24, 2007; 27(4): 909 - 918. [Abstract] [Full Text] [PDF]

				Y. Matsuda, A. Marzo, and S. Otani The presence of background dopamine signal converts long-term synaptic depression to potentiation in rat prefrontal cortex. J. Neurosci., May 3, 2006; 26(18): 4803 - 4810. [Abstract] [Full Text] [PDF]

				C.-C. Huang and K.-S. Hsu Presynaptic Mechanism Underlying cAMP-Induced Synaptic Potentiation in Medial Prefrontal Cortex Pyramidal Neurons Mol. Pharmacol., March 1, 2006; 69(3): 846 - 856. [Abstract] [Full Text] [PDF]

				A. Rodriguez, J. Whitson, and R. Granger Derivation and Analysis of Basic Computational Operations of Thalamocortical Circuits J. Cogn. Neurosci., June 1, 2004; 16(5): 856 - 877. [Abstract] [Full Text] [PDF]

				Y.-Y. Huang, E. Simpson, C. Kellendonk, and E. R. Kandel Genetic evidence for the bidirectional modulation of synaptic plasticity in the prefrontal cortex by D1 receptors PNAS, March 2, 2004; 101(9): 3236 - 3241. [Abstract] [Full Text] [PDF]

				M. A. LYNCH Long-Term Potentiation and Memory Physiol Rev, January 1, 2004; 84(1): 87 - 136. [Abstract] [Full Text] [PDF]

				S. Otani, H. Daniel, M.-P. Roisin, and F. Crepel Dopaminergic Modulation of Long-term Synaptic Plasticity in Rat Prefrontal Neurons Cereb Cortex, November 1, 2003; 13(11): 1251 - 1256. [Abstract] [Full Text] [PDF]

				S. Otani, H. Daniel, M. Takita, and F. Crepel Long-Term Depression Induced by Postsynaptic Group II Metabotropic Glutamate Receptors Linked to Phospholipase C and Intracellular Calcium Rises in Rat Prefrontal Cortex J. Neurosci., May 1, 2002; 22(9): 3434 - 3444. [Abstract] [Full Text] [PDF]

				E. H. Baeg, Y. B. Kim, J. Jang, H. T. Kim, I. Mook-Jung, and M. W. Jung Fast Spiking and Regular Spiking Neural Correlates of Fear Conditioning in the Medial Prefrontal Cortex of the Rat Cereb Cortex, May 1, 2001; 11(5): 441 - 451. [Abstract] [Full Text] [PDF]

				N. Auclair, S. Otani, P. Soubrie, and F. Crepel Cannabinoids Modulate Synaptic Strength and Plasticity at Glutamatergic Synapses of Rat Prefrontal Cortex Pyramidal Neurons J Neurophysiol, June 1, 2000; 83(6): 3287 - 3293. [Abstract] [Full Text] [PDF]

				C. M. Hempel, K. H. Hartman, X.-J. Wang, G. G. Turrigiano, and S. B. Nelson Multiple Forms of Short-Term Plasticity at Excitatory Synapses in Rat Medial Prefrontal Cortex J Neurophysiol, May 1, 2000; 83(5): 3031 - 3041. [Abstract] [Full Text] [PDF]

				S. Otani, N. Auclair, J.-M. Desce, M.-P. Roisin, and F. Crepel Dopamine Receptors and Groups I and II mGluRs Cooperate for Long-Term Depression Induction in Rat Prefrontal Cortex through Converging Postsynaptic Activation of MAP Kinases J. Neurosci., November 15, 1999; 19(22): 9788 - 9802. [Abstract] [Full Text] [PDF]

				C. Herry, R.-M. Vouimba, and R. Garcia Plasticity in the Mediodorsal Thalamo-Prefrontal Cortical Transmission in Behaving Mice J Neurophysiol, November 1, 1999; 82(5): 2827 - 2832. [Abstract] [Full Text] [PDF]

				X.-J. Wang Synaptic Basis of Cortical Persistent Activity: the Importance of NMDA Receptors to Working Memory J. Neurosci., November 1, 1999; 19(21): 9587 - 9603. [Abstract] [Full Text] [PDF]

				C. D. GILBERT Adult Cortical Dynamics Physiol Rev, April 1, 1998; 78(2): 467 - 485. [Abstract] [Full Text] [PDF]

				R. M. Vickery, S. H. Morris, and L. J. Bindman Metabotropic Glutamate Receptors Are Involved in Long-Term Potentiation in Isolated Slices of Rat Medial Frontal Cortex J Neurophysiol, December 1, 1997; 78(6): 3039 - 3046. [Abstract] [Full Text] [PDF]

				D. Wang and L. Maler In Vitro Plasticity of the Direct Feedback Pathway in the Electrosensory System of Apteronotus leptorhynchus J Neurophysiol, October 1, 1997; 78(4): 1882 - 1889. [Abstract] [Full Text] [PDF]

				F. Burette, T. M. Jay, and S. Laroche Reversal of LTP in the Hippocampal Afferent Fiber System to the Prefrontal Cortex In Vivo With Low-Frequency Patterns of Stimulation That Do Not Produce LTD J Neurophysiol, August 1, 1997; 78(2): 1155 - 1160. [Abstract] [Full Text] [PDF]