HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Adamson, R H Articles by Clough, G Search for Related Content PubMed PubMed Citation Articles by Adamson, R H Articles by Clough, G

Department of Physiology and Biophysics, St Mary's Hospital Medical School, London.

1. We have investigated the interaction of plasma proteins with the endothelial cell using cationized ferritin as a marker of the cell surface glycocalyx. 2. Single microvessels of the frog mesentery were sequentially perfused using glass micropipettes with solutions containing cationized ferritin (CF, 6.7 mg ml-1) in 0.10 M-NaCl and then with either frog plasma or bovine serum albumin (BSA; 50 or 10 mg ml-1), or protein-free Ringer solution, before suffusion fixation in 2.5% glutaraldehyde. 3. A layer of CF, usually two to four molecules thick, was associated with the luminal endothelial cell surface. In vessels post-flushed with protein-free Ringer solution the CF layer was closely adherent to all regions of the luminal endothelium, including the plasma membrane, vesicle diaphragms, coated pits and the entrances to clefts. However, when plasma was present during fixation the CF layer was separated from the cell surface by up to 100 nm over all regions. In vessels post-flushed with BSA the CF layer was also separated from the membrane but the effect was less striking. 4. The association of cationized ferritin with the endothelial cell surface was assessed quantitatively using electron micrographs of transverse sections (approximately 50 nm thick) of the perfused vessels, and expressed in terms of the depth of the layer of CF associated with the endothelial cell surface, its separation from the plasma membrane of the luminal endothelium, and the concentration of CF in the layer. The mean (+/- S.D.) separation in the presence of plasma, 32.3 +/- 10.5 nm (n = 12), was significantly greater (P less than 0.01) than that with either protein-free Ringer solution, 3.0 +/- 1.4 nm (n = 9), or BSA in Ringer solution, 8.3 +/- 3.0 nm (n = 8). The separation seen with BSA in Ringer was also significantly greater than that measured with a final Ringer solution perfusion (P less than 0.01). The effects of 10 and 50 mg ml-1 BSA were not different from one another. The total glycocalyx thickness, defined as the sum of the separation layer and depth of CF layer, with plasma present, 56.2 +/- 13.7 nm, was twice the value seen with Ringer solution, 28.0 +/- 9.1 nm (P less than 0.01), while the total thickness with BSA, 30.9 +/- 5.4 nm, was not different from the Ringer solution value.(ABSTRACT TRUNCATED AT 400 WORDS)

This article has been cited by other articles:

				J. W. G. E. VanTeeffelen, A. A. Constantinescu, J. Brands, J. A. E. Spaan, and H. Vink Bradykinin- and sodium nitroprusside-induced increases in capillary tube haematocrit in mouse cremaster muscle are associated with impaired glycocalyx barrier properties J. Physiol., July 1, 2008; 586(13): 3207 - 3218. [Abstract] [Full Text] [PDF]

				A. I. Barakat Dragging Along: The Glycocalyx and Vascular Endothelial Cell Mechanotransduction Circ. Res., April 11, 2008; 102(7): 747 - 748. [Full Text] [PDF]

				B. Haraldsson, J. Nystrom, and W. M. Deen Properties of the Glomerular Barrier and Mechanisms of Proteinuria Physiol Rev, April 1, 2008; 88(2): 451 - 487. [Abstract] [Full Text] [PDF]

				M. Rehm, D. Bruegger, F. Christ, P. Conzen, M. Thiel, M. Jacob, D. Chappell, M. Stoeckelhuber, U. Welsch, B. Reichart, et al. Shedding of the Endothelial Glycocalyx in Patients Undergoing Major Vascular Surgery With Global and Regional Ischemia Circulation, October 23, 2007; 116(17): 1896 - 1906. [Abstract] [Full Text] [PDF]

				A. P. Stevens, V. Hlady, and R. O. Dull Fluorescence correlation spectroscopy can probe albumin dynamics inside lung endothelial glycocalyx Am J Physiol Lung Cell Mol Physiol, August 1, 2007; 293(2): L328 - L335. [Abstract] [Full Text] [PDF]

				J. W.G.E. VanTeeffelen, J. Brands, C. Jansen, J. A.E. Spaan, and H. Vink Heparin Impairs Glycocalyx Barrier Properties and Attenuates Shear Dependent Vasodilation in Mice Hypertension, July 1, 2007; 50(1): 261 - 267. [Abstract] [Full Text] [PDF]

				P. M. A. van Haaren, E. VanBavel, H. Vink, and J. A. E. Spaan Charge modification of the endothelial surface layer modulates the permeability barrier of isolated rat mesenteric small arteries Am J Physiol Heart Circ Physiol, December 1, 2005; 289(6): H2503 - H2507. [Abstract] [Full Text] [PDF]

				M. M. Thi, J. M. Tarbell, S. Weinbaum, and D. C. Spray The role of the glycocalyx in reorganization of the actin cytoskeleton under fluid shear stress: A "bumper-car" model PNAS, November 23, 2004; 101(47): 16483 - 16488. [Abstract] [Full Text] [PDF]

				A. Sasse, Z. Ding, M. Wallich, A. Godecke, and J. Schrader Vascular transfer of adenovirus is augmented by nitric oxide in the rat heart Am J Physiol Heart Circ Physiol, September 1, 2004; 287(3): H1362 - H1368. [Abstract] [Full Text] [PDF]

				A. W. Mulivor and H. H. Lipowsky Inflammation- and ischemia-induced shedding of venular glycocalyx Am J Physiol Heart Circ Physiol, May 1, 2004; 286(5): H1672 - H1680. [Abstract] [Full Text] [PDF]

				S. H. Platts and B. R. Duling Adenosine A3 Receptor Activation Modulates the Capillary Endothelial Glycocalyx Circ. Res., January 9, 2004; 94(1): 77 - 82. [Abstract] [Full Text] [PDF]

				B. Rippe What is the role of albumin in proteinuric glomerulopathies? Nephrol. Dial. Transplant., January 1, 2004; 19(1): 1 - 5. [Full Text] [PDF]

				M. R. Kellen and J. B. Bassingthwaighte An integrative model of coupled water and solute exchange in the heart Am J Physiol Heart Circ Physiol, August 7, 2003; 285(3): H1303 - H1316. [Abstract] [Full Text] [PDF]

				B. M. Mazzag, J. S. Tamaresis, and A. I. Barakat A Model for Shear Stress Sensing and Transmission in Vascular Endothelial Cells Biophys. J., June 1, 2003; 84(6): 4087 - 4101. [Abstract] [Full Text] [PDF]

				B. M. Fu and S. Shen Structural mechanisms of acute VEGF effect on microvessel permeability Am J Physiol Heart Circ Physiol, June 1, 2003; 284(6): H2124 - H2135. [Abstract] [Full Text] [PDF]

				B. M. Fu, B. Chen, and W. Chen An electrodiffusion model for effects of surface glycocalyx layer on microvessel permeability Am J Physiol Heart Circ Physiol, April 1, 2003; 284(4): H1240 - H1250. [Abstract] [Full Text] [PDF]

				J. Sorensson, A. Bjornson, M. Ohlson, B. J. Ballermann, and B. Haraldsson Synthesis of sulfated proteoglycans by bovine glomerular endothelial cells in culture Am J Physiol Renal Physiol, February 1, 2003; 284(2): F373 - F380. [Abstract] [Full Text] [PDF]

				A. A. Constantinescu, H. Vink, and J. A. E. Spaan Elevated capillary tube hematocrit reflects degradation of endothelial cell glycocalyx by oxidized LDL Am J Physiol Heart Circ Physiol, March 1, 2001; 280(3): H1051 - H1057. [Abstract] [Full Text] [PDF]

				X. Hu, R. H. Adamson, B. Liu, F. E. Curry, and S. Weinbaum Starling forces that oppose filtration after tissue oncotic pressure is increased Am J Physiol Heart Circ Physiol, October 1, 2000; 279(4): H1724 - H1736. [Abstract] [Full Text] [PDF]

				V. H. Huxley and D. A. Williams Role of a glycocalyx on coronary arteriole permeability to proteins: evidence from enzyme treatments Am J Physiol Heart Circ Physiol, April 1, 2000; 278(4): H1177 - H1185. [Abstract] [Full Text] [PDF]

				C. B. S. Henry and B. R. Duling Permeation of the luminal capillary glycocalyx is determined by hyaluronan Am J Physiol Heart Circ Physiol, August 1, 1999; 277(2): H508 - H514. [Abstract] [Full Text] [PDF]

				C. C. Michel and F. E. Curry Microvascular Permeability Physiol Rev, July 1, 1999; 79(3): 703 - 761. [Abstract] [Full Text] [PDF]

				J. Sorensson, G. L. Matejka, M. Ohlson, and B. Haraldsson Human endothelial cells produce orosomucoid, an important component of the capillary barrier Am J Physiol Heart Circ Physiol, February 1, 1999; 276(2): H530 - H534. [Abstract] [Full Text] [PDF]

				J. R. Lindner, S. Ismail, W. D. Spotnitz, D. M. Skyba, A. R. Jayaweera, and S. Kaul Albumin Microbubble Persistence During Myocardial Contrast Echocardiography Is Associated With Microvascular Endothelial Glycocalyx Damage Circulation, November 17, 1998; 98(20): 2187 - 2194. [Abstract] [Full Text] [PDF]

				H. Vink and B. R. Duling Identification of Distinct Luminal Domains for Macromolecules, Erythrocytes, and Leukocytes Within Mammalian Capillaries Circ. Res., September 1, 1996; 79(3): 581 - 589. [Abstract] [Full Text]