HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Shirasaki, T Articles by Akaike, N Search for Related Content PubMed PubMed Citation Articles by Shirasaki, T Articles by Akaike, N

Department of Neurophysiology, Tohoku University School of Medicine, Sendai, Japan.

1. Effect of intracellular ATP on Cl- current (ICl) mediated by the GABA (gamma-aminobutyric acid) receptor subtype, GABAA, was studied in dissociated nucleus tractus solitarii (NTS) neurones using the whole-cell mode of patch clamp. A concentration-jump technique termed 'Y tube' was used to rapidly apply agents externally. Dissociated neurones were obtained from 1- to 3-week-old rats. 2. When the patch-pipette solution contained 2 mM-ATP, the amplitude of ICl elicited by 10(-5) M-GABA did not show any time-dependent decrease (apparent run-down), for more than 60 min after the initial recording. In the presence of ATP, the half-maximum concentration (KD) and Hill coefficient calculated from the GABA concentration-response curve were 9.12 microM and 1.47, respectively. 3. In the absence of intracellular ATP, the amplitude of GABA-induced ICl decreased with time. The relative peak amplitudes after 20 and 60 min from the initial recording were 0.40 +/- 0.09 (n = 11) and 0.16 +/- 0.05 (n = 8) with respect to the initial response. 4. Removal of Mg2+ from the internal solution induced run-down of the GABA response even in the presence of 2 mM-intracellular ATP, suggesting that both intracellular ATP (2 mM or more) and Mg2+ are necessary to prevent run-down of the GABA response. 5. Activation of dephosphorylation processes by alkaline phosphatase (100-200 microM) did not affect the GABA response in neurones perfused with internal solution containing 2 mM-ATP and 3 mM-Mg2+. Blocking the dephosphorylation process by okadaic acid, a phosphatase inhibitor, did not prevent the run-down of the GABA response. 6. Calcium influxes passing through both the voltage-dependent L-type Ca2+ channel and the glutamate receptor-operated cation channel did not affect ICl induced by GABA. 7. GABA-induced ICl was also maintained by adding 2 mM-ADP or ATP gamma S (adenosine-5'-O-3-thiotriphosphate) to the internal solution containing Mg2+. Addition of 2 mM-adenosine, AMP, cyclic AMP, AMP-PNP (adenylimido-diphosphate) or ADP beta S (adenosine-5'-O-2-thiodiphosphate) to the internal solution did not prevent the run-down of the GABA response even in the presence of 3 mM-intracellular Mg2+. Based on the chemical specificity of these ATP analogues, it is suggested that there is an ATP-sensitive binding site (ATP receptor) in the cytoplasmic side of the cell membrane.(ABSTRACT TRUNCATED AT 400 WORDS)

This article has been cited by other articles:

				C. Xiao, C. Zhou, K. Li, and J.-H. Ye Presynaptic GABAA receptors facilitate GABAergic transmission to dopaminergic neurons in the ventral tegmental area of young rats J. Physiol., May 1, 2007; 580(3): 731 - 743. [Abstract] [Full Text] [PDF]

				G. Tolstykh, S. Belugin, O. Tolstykh, and S. Mifflin Responses to GABAA Receptor Activation Are Altered in NTS Neurons Isolated From Renal-Wrap Hypertensive Rats Hypertension, October 1, 2003; 42(4): 732 - 736. [Abstract] [Full Text] [PDF]

				I.-S. Jang, H.-J. Jeong, and N. Akaike Contribution of the Na-K-Cl Cotransporter on GABAA Receptor-Mediated Presynaptic Depolarization in Excitatory Nerve Terminals J. Neurosci., August 15, 2001; 21(16): 5962 - 5972. [Abstract] [Full Text] [PDF]

				J. Kehoe and C. Vulfius Independence of and Interactions between GABA-, Glutamate-, and Acetylcholine-Activated Cl Conductances in Aplysia Neurons J. Neurosci., December 1, 2000; 20(23): 8585 - 8596. [Abstract] [Full Text] [PDF]

				I. C. Solomon, N. H. Edelman, and J. A. Neubauer Pre-Botzinger Complex Functions as a Central Hypoxia Chemosensor for Respiration In Vivo J Neurophysiol, May 1, 2000; 83(5): 2854 - 2868. [Abstract] [Full Text] [PDF]

				R.-Q. Huang and G. H. Dillon Maintenance of Recombinant Type A gamma -Aminobutyric Acid Receptor Function: Role of Protein Tyrosine Phosphorylation and Calcineurin J. Pharmacol. Exp. Ther., July 1, 1998; 286(1): 243 - 255. [Abstract] [Full Text]