| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Department of Zoology and Genetics, Iowa State University, Ames 50011-3223, USA.
1. The nystatin perforated-patch method was used to record macroscopic currents from anti-trinitrophenyl (TNP) immunoglobulin E (IgE)-sensitized rat basophilic leukaemia (RBL-2H3) cells at 37 degrees C. 2. An inwardly rectifying Ca2+ current (ICa) was activated upon stimulation with the multivalent antigen trinitrophenylated bovine serum albumin (TNP-BSA). Induction of ICa was not observed at room temperature. ICa was reversed and reinduced upon cyclical addition of the monovalent hapten dinitrophenyl (DNP)-lysine and multivalent antigen, indicating that a specific interaction of antigen with IgE was required to elicit ICa. 3. The antigen-induced current was also carried by Ba2+ or Sr2+, and to a lesser extent by Na+, in the nominal absence of Ca2+. ICa did not exhibit time-dependent opening (< or = 1 ms) in response to hyperpolarizing voltage steps to -100 mV, although it did accumulate steady-state inactivation of approximately 40-50% over 100 ms. 4. Two inorganic blockers of antigen-stimulated 45Ca2+ influx and secretion, La3+ and Zn2+, inhibited ICa by approximately 50% at concentrations known to produce 50% block of 45Ca2+ influx. In contrast, cromolyn sodium (0.5 mM) and the L-type Ca2+ channel antagonist nitrendipine (5 microM) had no effect on ICa. 5. ICa also was induced by the intracellular Ca2+ mobilizer thapsigargin. Because the actions of thapsigargin and antigen were not additive, IgE receptor cross-linkage appears to activate the recently described capacitative Ca2+ entry channels.
This article has been cited by other articles:
|
|
 |
|
  N. Calloway, M. Vig, J.-P. Kinet, D. Holowka, and B. Baird Molecular Clustering of STIM1 with Orai1/CRACM1 at the Plasma Membrane Depends Dynamically on Depletion of Ca2+ Stores and on Electrostatic Interactions Mol. Biol. Cell, January 1, 2009; 20(1): 389 - 399. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Musset, D. Morgan, V. V. Cherny, D. W. MacGlashan Jr, L. L. Thomas, E. Rios, and T. E. DeCoursey A pH-stabilizing role of voltage-gated proton channels in IgE-mediated activation of human basophils PNAS, August 5, 2008; 105(31): 11020 - 11025. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Ishizaki, M. Iigo, N. Yamamoto, and Y. Oka Different Modes of Gonadotropin-Releasing Hormone (GnRH) Release from Multiple GnRH Systems as Revealed by Radioimmunoassay Using Brain Slices of a Teleost, the Dwarf Gourami (Colisa lalia) Endocrinology, April 1, 2004; 145(4): 2092 - 2103. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Li, J.-Y. Sul, and P. G. Haydon A Calcium-Induced Calcium Influx Factor, Nitric Oxide, Modulates the Refilling of Calcium Stores in Astrocytes J. Neurosci., November 12, 2003; 23(32): 10302 - 10310. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Y. Estevez, R. K. Roberts, and K. Strange Identification of Store-independent and Store-operated Ca2+ Conductances in Caenorhabditis elegans Intestinal Epithelial Cells J. Gen. Physiol., July 28, 2003; 122(2): 207 - 223. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z.-H. Xie, I. Ambudkar, and R. P. Siraganian The Adapter Molecule Gab2 Regulates Fc{epsilon}RI-Mediated Signal Transduction in Mast Cells J. Immunol., May 1, 2002; 168(9): 4682 - 4691. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. F. Fomina, C. M. Fanger, J. A. Kozak, and M. D. Cahalan Single Channel Properties and Regulated Expression of Ca2+ Release-Activated Ca2+ (CRAC) Channels in Human T Cells J. Cell Biol., September 18, 2000; 150(6): 1435 - 1444. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. R. Ehring, H. H. Kerschbaum, C. M. Fanger, C. Eder, H. Rauer, and M. D. Cahalan Vanadate Induces Calcium Signaling, Ca2+ Release-Activated Ca2+ Channel Activation, and Gene Expression in T Lymphocytes and RBL-2H3 Mast Cells Via Thiol Oxidation J. Immunol., January 15, 2000; 164(2): 679 - 687. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. McCloskey and L. Zhang Potentiation of Fc{isin} Receptor I-activated Ca2+ Current (ICRAC) by Cholera Toxin: Possible Mediation by ADP Ribosylation Factor J. Cell Biol., January 10, 2000; 148(1): 137 - 146. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. M. Broad, D. L. Armstrong, and J. W. Putney Jr. Role of the Inositol 1,4,5-Trisphosphate Receptor in Ca2+ Feedback Inhibition of Calcium Release-activated Calcium Current (Icrac) J. Biol. Chem., November 12, 1999; 274(46): 32881 - 32888. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. F. Fomina and M. C. Nowycky A Current Activated on Depletion of Intracellular Ca2+ Stores Can Regulate Exocytosis in Adrenal Chromaffin Cells J. Neurosci., May 15, 1999; 19(10): 3711 - 3722. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. REINHEIMER, D. BAUMGÄRTNER, K.-D. HÖHLE, K. RACKÉ, and I. WESSLER Acetylcholine via Muscarinic Receptors Inhibits Histamine Release from Human Isolated Bronchi Am. J. Respir. Crit. Care Med., July 1, 1997; 156(2): 389 - 395. [Abstract] [Full Text]
|
|
|
|
|
|
N. Djouder, U. Prepens, K. Aktories, and A. Cavalie Inhibition of Calcium Release-activated Calcium Current by Rac/Cdc42-inactivating Clostridial Cytotoxins in RBL Cells J. Biol. Chem., June 16, 2000; 275(25): 18732 - 18738. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. J. Smith, Z. Surviladze, E. A. Gaudet, J. M. Backer, C. A. Mitchell, and B. S. Wilson p110beta and p110delta Phosphatidylinositol 3-Kinases Up-regulate Fcepsilon RI-activated Ca2+ Influx by Enhancing Inositol 1,4,5-Trisphosphate Production J. Biol. Chem., May 11, 2001; 276(20): 17213 - 17220. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
