HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Markram, H Articles by Sakmann, B Search for Related Content PubMed PubMed Citation Articles by Markram, H Articles by Sakmann, B

Max-Planck-Institut für medizinische Forschung, Heidelberg, Germany.

1. Dendrites of rat neocortical layer V pyramidal neurons were loaded with the Ca2+ indicator dye Calcium Green-1 (CG-1) or fluo-3, and the mechanisms which govern action potential (AP)-evoked transient changes in dendritic cytosolic Ca2+ concentration ([Ca2+]i) were examined. APs were initiated either by synaptic stimulation or by depolarizing the soma or dendrite by current injection, and changes in fluorescence of the indicator dye were measured in the proximal 170 microns of the apical dendrite. 2. Simultaneous two-pipette recordings of APs from the soma and apical dendrite, and dendritic fluorescence imaging indicated that a single AP propagating from the soma into the apical dendrite evokes a rapid transient increase in fluorescence indicating a transient increase in [Ca2+]i. At 35-37 degrees C the decay time constant of the fluorescence transient following an AP was around 80 ms. 3. Voltage-activated Ca2+ channels (VACCs) of several subtypes mediated the AP-evoked fluorescence transient in the proximal (100-170 microns) apical dendrite. The AP-evoked fluorescence transient resulted from Ca2+ entry through L-type (nifedipine sensitive; 25%), N-type (omega-conotoxin GVIA sensitive; 28%) and P-type (omega-agatoxin IVA sensitive; 10%) Ca2+ channels and through Ca2+ channels (R-type) not sensitive to L-, N- and P-type Ca2+ channel blockers (cadmium ion sensitive; 37%). 4. The decay time course of the dendritic fluorescence transient was prolonged by the blockers of endoplasmic reticulum (ER) Ca(2+)-ATPase, cyclopiazonic acid and thapsigargin, suggesting that uptake of Ca2+ into the ER in dendrites governs clearance of dendritic Ca2+. 5. The decay time course of the fluorescence transient was slightly prolonged by benzamil, a blocker of plasma membrane Na(+)-Ca2+ exchange and by calmidazolium, a blocker of the calmodulin-dependent plasma membrane Ca(2+)-ATPase, suggesting that these pathways are less important for dendrite Ca2+ clearance following a single AP. Neither the mitochondrial uncoupler carbonyl cyanide p-(trifluoromethoxy)phenylhydrazone (FCCP) nor the blocker of Ca2+ uptake into mitochondria, Ruthenium Red, had any measurable effect on the decay time course of the fluorescence transient. 6. Dendritic fluorescence transients measured during trains of dendritic APs began to summate at impulse frequencies of 5 APs s-1. At higher frequencies APs caused a concerted and maintained elevation of dendritic fluorescence during the train.(ABSTRACT TRUNCATED AT 400 WORDS)

This article has been cited by other articles:

				T. Sasaki, N. Takahashi, N. Matsuki, and Y. Ikegaya Fast and Accurate Detection of Action Potentials From Somatic Calcium Fluctuations J Neurophysiol, September 1, 2008; 100(3): 1668 - 1676. [Abstract] [Full Text] [PDF]

				G. Stocca, C. Schmidt-Hieber, and J. Bischofberger Differential dendritic Ca2+ signalling in young and mature hippocampal granule cells J. Physiol., August 15, 2008; 586(16): 3795 - 3811. [Abstract] [Full Text] [PDF]

				X. Yu, H. Z. Shouval, and J. J. Knierim A Biophysical Model of Synaptic Plasticity and Metaplasticity Can Account for the Dynamics of the Backward Shift of Hippocampal Place Fields J Neurophysiol, August 1, 2008; 100(2): 983 - 992. [Abstract] [Full Text] [PDF]

				N. Kuczewski, C. Porcher, N. Ferrand, H. Fiorentino, C. Pellegrino, R. Kolarow, V. Lessmann, I. Medina, and J.-L. Gaiarsa Backpropagating Action Potentials Trigger Dendritic Release of BDNF during Spontaneous Network Activity J. Neurosci., July 2, 2008; 28(27): 7013 - 7023. [Abstract] [Full Text] [PDF]

				K.-H. Cho, H.-J. Jang, E.-H. Lee, S. H. Yoon, S. J. Hahn, Y.-H. Jo, M.-S. Kim, and D.-J. Rhie Differential Cholinergic Modulation of Ca2+ Transients Evoked by Backpropagating Action Potentials in Apical and Basal Dendrites of Cortical Pyramidal Neurons J Neurophysiol, June 1, 2008; 99(6): 2833 - 2843. [Abstract] [Full Text] [PDF]

				B. A. Graham, A. M. Brichta, and R. J. Callister Recording Temperature Affects the Excitability of Mouse Superficial Dorsal Horn Neurons, In Vitro J Neurophysiol, May 1, 2008; 99(5): 2048 - 2059. [Abstract] [Full Text] [PDF]

				J. M. Power and P. Sah Competition between Calcium-Activated K+ Channels Determines Cholinergic Action on Firing Properties of Basolateral Amygdala Projection Neurons J. Neurosci., March 19, 2008; 28(12): 3209 - 3220. [Abstract] [Full Text] [PDF]

				D. F. de Sevilla, A. Nunez, M. Borde, R. Malinow, and W. Buno Cholinergic-Mediated IP3-Receptor Activation Induces Long-Lasting Synaptic Enhancement in CA1 Pyramidal Neurons J. Neurosci., February 6, 2008; 28(6): 1469 - 1478. [Abstract] [Full Text] [PDF]

				M. Teagarden, J. F. Atherton, M. D. Bevan, and C. J. Wilson Accumulation of cytoplasmic calcium, but not apamin-sensitive afterhyperpolarization current, during high frequency firing in rat subthalamic nucleus cells J. Physiol., February 1, 2008; 586(3): 817 - 833. [Abstract] [Full Text] [PDF]

				T. Rinaldi, K. Kulangara, K. Antoniello, and H. Markram Elevated NMDA receptor levels and enhanced postsynaptic long-term potentiation induced by prenatal exposure to valproic acid PNAS, August 14, 2007; 104(33): 13501 - 13506. [Abstract] [Full Text] [PDF]

				B.-J. Lin, T.-W. Chen, and D. Schild Cell type-specific relationships between spiking and [Ca2+]i in neurons of the Xenopus tadpole olfactory bulb J. Physiol., July 1, 2007; 582(1): 163 - 175. [Abstract] [Full Text] [PDF]

				J. M. Power and P. Sah Distribution of IP3-mediated calcium responses and their role in nuclear signalling in rat basolateral amygdala neurons J. Physiol., May 1, 2007; 580(3): 835 - 857. [Abstract] [Full Text] [PDF]

				L. Chen, J. D. Bohanick, M. Nishihara, J. K. Seamans, and C. R. Yang Dopamine D1/5 Receptor-Mediated Long-Term Potentiation of Intrinsic Excitability in Rat Prefrontal Cortical Neurons: Ca2+-Dependent Intracellular Signaling J Neurophysiol, March 1, 2007; 97(3): 2448 - 2464. [Abstract] [Full Text] [PDF]

				C. L. Jasoni, M. G. Todman, M. M. Strumia, and A. E. Herbison Cell Type-Specific Expression of a Genetically Encoded Calcium Indicator Reveals Intrinsic Calcium Oscillations in Adult Gonadotropin-Releasing Hormone Neurons J. Neurosci., January 24, 2007; 27(4): 860 - 867. [Abstract] [Full Text] [PDF]

				A. Lorincz, B. Rozsa, G. Katona, E. S. Vizi, and G. Tamas Differential distribution of NCX1 contributes to spine-dendrite compartmentalization in CA1 pyramidal cells PNAS, January 16, 2007; 104(3): 1033 - 1038. [Abstract] [Full Text] [PDF]

				S. L. Mironov and N. Symonchuk ER vesicles and mitochondria move and communicate at synapses J. Cell Sci., December 1, 2006; 119(23): 4926 - 4934. [Abstract] [Full Text] [PDF]

				V. Scheuss, R. Yasuda, A. Sobczyk, and K. Svoboda Nonlinear [Ca2+] Signaling in Dendrites and Spines Caused by Activity-Dependent Depression of Ca2+ Extrusion J. Neurosci., August 2, 2006; 26(31): 8183 - 8194. [Abstract] [Full Text] [PDF]

				M. Isokawa and B. E. Alger Ryanodine Receptor Regulates Endogenous Cannabinoid Mobilization in the Hippocampus J Neurophysiol, May 1, 2006; 95(5): 3001 - 3011. [Abstract] [Full Text] [PDF]

				S. Andjelic and V. Torre Calcium Dynamics and Compartmentalization in Leech Neurons J Neurophysiol, December 1, 2005; 94(6): 4430 - 4440. [Abstract] [Full Text] [PDF]

				M. Rosanova and D. Ulrich Pattern-Specific Associative Long-Term Potentiation Induced by a Sleep Spindle-Related Spike Train J. Neurosci., October 12, 2005; 25(41): 9398 - 9405. [Abstract] [Full Text] [PDF]

				M. A. L Nicolelis Computing with thalamocortical ensembles during different behavioural states J. Physiol., July 1, 2005; 566(1): 37 - 47. [Abstract] [Full Text] [PDF]

				J. C. F. Lee, J. C. Callaway, and R. C. Foehring Effects of Temperature on Calcium Transients and Ca2+-Dependent Afterhyperpolarizations in Neocortical Pyramidal Neurons J Neurophysiol, April 1, 2005; 93(4): 2012 - 2020. [Abstract] [Full Text] [PDF]

				R. D. Traub, D. Contreras, M. O. Cunningham, H. Murray, F. E. N. LeBeau, A. Roopun, A. Bibbig, W. B. Wilent, M. J. Higley, and M. A. Whittington Single-Column Thalamocortical Network Model Exhibiting Gamma Oscillations, Sleep Spindles, and Epileptogenic Bursts J Neurophysiol, April 1, 2005; 93(4): 2194 - 2232. [Abstract] [Full Text] [PDF]

				J. M. Power and P. Sah Intracellular calcium store filling by an L-type calcium current in the basolateral amygdala at subthreshold membrane potentials J. Physiol., January 15, 2005; 562(2): 439 - 453. [Abstract] [Full Text] [PDF]

				W. W. Wu, C. S. Chan, and J. F. Disterhoft Slow Afterhyperpolarization Governs the Development of NMDA Receptor-Dependent Afterdepolarization in CA1 Pyramidal Neurons During Synaptic Stimulation J Neurophysiol, October 1, 2004; 92(4): 2346 - 2356. [Abstract] [Full Text] [PDF]

				B. A. Milojkovic, M. S. Radojicic, t. l. P. S. Goldman-Rakic, and S. D. Antic Burst generation in rat pyramidal neurones by regenerative potentials elicited in a restricted part of the basilar dendritic tree J. Physiol., July 1, 2004; 558(1): 193 - 211. [Abstract] [Full Text] [PDF]

				A. Kumar and T. C. Foster Enhanced Long-Term Potentiation During Aging Is Masked by Processes Involving Intracellular Calcium Stores J Neurophysiol, June 1, 2004; 91(6): 2437 - 2444. [Abstract] [Full Text] [PDF]

				T. Nevian and B. Sakmann Single Spine Ca2+ Signals Evoked by Coincident EPSPs and Backpropagating Action Potentials in Spiny Stellate Cells of Layer 4 in the Juvenile Rat Somatosensory Barrel Cortex J. Neurosci., February 18, 2004; 24(7): 1689 - 1699. [Abstract] [Full Text] [PDF]

				H. J. Abel, J.C.F. Lee, J. C. Callaway, and R. C. Foehring Relationships Between Intracellular Calcium and Afterhyperpolarizations in Neocortical Pyramidal Neurons J Neurophysiol, January 1, 2004; 91(1): 324 - 335. [Abstract] [Full Text]

				M.-H. Kim, S.-h. Lee, K. H. Park, W.-K. Ho, and S.-H. Lee Distribution of K+-Dependent Na+/Ca2+ Exchangers in the Rat Supraoptic Magnocellular Neuron Is Polarized to Axon Terminals J. Neurosci., December 17, 2003; 23(37): 11673 - 11680. [Abstract] [Full Text] [PDF]

				T. Berger, W. Senn, and H.-R. Luscher Hyperpolarization-Activated Current Ih Disconnects Somatic and Dendritic Spike Initiation Zones in Layer V Pyramidal Neurons J Neurophysiol, October 1, 2003; 90(4): 2428 - 2437. [Abstract] [Full Text] [PDF]

				M. CHESLER Regulation and Modulation of pH in the Brain Physiol Rev, October 1, 2003; 83(4): 1183 - 1221. [Abstract] [Full Text] [PDF]

				V. Egger, K. Svoboda, and Z. F. Mainen Mechanisms of Lateral Inhibition in the Olfactory Bulb: Efficiency and Modulation of Spike-Evoked Calcium Influx into Granule Cells J. Neurosci., August 20, 2003; 23(20): 7551 - 7558. [Abstract] [Full Text] [PDF]

				M. Eder, K. Becker, G. Rammes, A. Schierloh, S. C. Azad, W. Zieglgansberger, and H.-U. Dodt Distribution and Properties of Functional Postsynaptic Kainate Receptors on Neocortical Layer V Pyramidal Neurons J. Neurosci., July 23, 2003; 23(16): 6660 - 6670. [Abstract] [Full Text] [PDF]

				S. C. Molitor and P. B. Manis Dendritic Ca2+ Transients Evoked by Action Potentials in Rat Dorsal Cochlear Nucleus Pyramidal and Cartwheel Neurons J Neurophysiol, April 1, 2003; 89(4): 2225 - 2237. [Abstract] [Full Text] [PDF]

				Y. Schiller Inter-Ictal- and Ictal-Like Epileptic Discharges in the Dendritic Tree of Neocortical Pyramidal Neurons J Neurophysiol, December 1, 2002; 88(6): 2954 - 2962. [Abstract] [Full Text] [PDF]

				M. E. Larkum and J. J. Zhu Signaling of Layer 1 and Whisker-Evoked Ca2+ and Na+ Action Potentials in Distal and Terminal Dendrites of Rat Neocortical Pyramidal Neurons In Vitro and In Vivo J. Neurosci., August 15, 2002; 22(16): 6991 - 7005. [Abstract] [Full Text] [PDF]

				H. Z. Shouval, M. F. Bear, and L. N Cooper A unified model of NMDA receptor-dependent bidirectional synaptic plasticity PNAS, August 6, 2002; 99(16): 10831 - 10836. [Abstract] [Full Text] [PDF]

				Z. Xiang, J. R. Huguenard, and D. A. Prince Synaptic Inhibition of Pyramidal Cells Evoked by Different Interneuronal Subtypes in Layer V of Rat Visual Cortex J Neurophysiol, August 1, 2002; 88(2): 740 - 750. [Abstract] [Full Text] [PDF]

				J. M. Power and P. Sah Nuclear Calcium Signaling Evoked by Cholinergic Stimulation in Hippocampal CA1 Pyramidal Neurons J. Neurosci., May 1, 2002; 22(9): 3454 - 3462. [Abstract] [Full Text] [PDF]

				Y. Liang, L.-L. Yuan, D. Johnston, and R. Gray Calcium Signaling at Single Mossy Fiber Presynaptic Terminals in the Rat Hippocampus J Neurophysiol, February 1, 2002; 87(2): 1132 - 1137. [Abstract] [Full Text] [PDF]

				S. Okabe, T. Urushido, D. Konno, H. Okado, and K. Sobue Rapid Redistribution of the Postsynaptic Density Protein PSD-Zip45 (Homer 1c) and Its Differential Regulation by NMDA Receptors and Calcium Channels J. Neurosci., December 15, 2001; 21(24): 9561 - 9571. [Abstract] [Full Text] [PDF]

				A. Frick, W. Zieglgansberger, and H.-U. Dodt Glutamate Receptors Form Hot Spots on Apical Dendrites of Neocortical Pyramidal Neurons J Neurophysiol, September 1, 2001; 86(3): 1412 - 1421. [Abstract] [Full Text] [PDF]

				J. C. Oakley, P. C. Schwindt, and W. E. Crill Initiation and Propagation of Regenerative Ca2+-Dependent Potentials in Dendrites of Layer 5 Pyramidal Neurons J Neurophysiol, July 1, 2001; 86(1): 503 - 513. [Abstract] [Full Text] [PDF]

				C. R. Rose and A. Konnerth NMDA Receptor-Mediated Na+ Signals in Spines and Dendrites J. Neurosci., June 15, 2001; 21(12): 4207 - 4214. [Abstract] [Full Text] [PDF]

				T. Inoue, X. Lin, K. A. Kohlmeier, H. T. Orr, H. Y. Zoghbi, and W. N. Ross Calcium Dynamics and Electrophysiological Properties of Cerebellar Purkinje Cells in SCA1 Transgenic Mice J Neurophysiol, April 1, 2001; 85(4): 1750 - 1760. [Abstract] [Full Text] [PDF]

				N. Maurice, T. Tkatch, M. Meisler, L. K. Sprunger, and D. J. Surmeier D1/D5 Dopamine Receptor Activation Differentially Modulates Rapidly Inactivating and Persistent Sodium Currents in Prefrontal Cortex Pyramidal Neurons J. Neurosci., April 1, 2001; 21(7): 2268 - 2277. [Abstract] [Full Text] [PDF]

				M. Häusser, N. Spruston, and G. J. Stuart Diversity and Dynamics of Dendritic Signaling Science, October 27, 2000; 290(5492): 739 - 744. [Abstract] [Full Text]

				L. S. Dove, S.-S. Nahm, D. Murchison, L. C. Abbott, and W. H. Griffith Altered Calcium Homeostasis in Cerebellar Purkinje Cells of Leaner Mutant Mice J Neurophysiol, July 1, 2000; 84(1): 513 - 524. [Abstract] [Full Text] [PDF]

				H. Tsubokawa, S. Offermanns, M. Simon, and M. Kano Calcium-Dependent Persistent Facilitation of Spike Backpropagation in the CA1 Pyramidal Neurons J. Neurosci., July 1, 2000; 20(13): 4878 - 4884. [Abstract] [Full Text] [PDF]

				A. Majewska, E. Brown, J. Ross, and R. Yuste Mechanisms of Calcium Decay Kinetics in Hippocampal Spines: Role of Spine Calcium Pumps and Calcium Diffusion through the Spine Neck in Biochemical Compartmentalization J. Neurosci., March 1, 2000; 20(5): 1722 - 1734. [Abstract] [Full Text] [PDF]

				Y. Kovalchuk, J. Eilers, J. Lisman, and A. Konnerth NMDA Receptor-Mediated Subthreshold Ca2+ Signals in Spines of Hippocampal Neurons J. Neurosci., March 1, 2000; 20(5): 1791 - 1799. [Abstract] [Full Text] [PDF]

				V. N. Murthy, T. J. Sejnowski, and C. F. Stevens Dynamics of dendritic calcium transients evoked by quantal release at excitatory hippocampal synapses PNAS, January 18, 2000; 97(2): 901 - 906. [Abstract] [Full Text] [PDF]

				M. E. Larkum, K. M. M. Kaiser, and B. Sakmann Calcium electrogenesis in distal apical dendrites of layer 5 pyramidal cells at a critical frequency of back-propagating action potentials PNAS, December 7, 1999; 96(25): 14600 - 14604. [Abstract] [Full Text] [PDF]

				V. Durr and M. Egelhaaf In Vivo Calcium Accumulation in Presynaptic and Postsynaptic Dendrites of Visual Interneurons J Neurophysiol, December 1, 1999; 82(6): 3327 - 3338. [Abstract] [Full Text] [PDF]

				S. E. Smith and M. Chesler Effect of Divalent Cations on AMPA-Evoked Extracellular Alkaline Shifts in Rat Hippocampal Slices J Neurophysiol, October 1, 1999; 82(4): 1902 - 1908. [Abstract] [Full Text] [PDF]

				V. Sourdet and D. Debanne The Role of Dendritic Filtering in Associative Long-Term Synaptic Plasticity Learn. Mem., September 1, 1999; 6(5): 422 - 447. [Abstract] [Full Text]

				F. Mammano, G. I. Frolenkov, L. Lagostena, I. A. Belyantseva, M. Kurc, V. Dodane, A. Colavita, and B. Kachar ATP-Induced Ca2+ Release in Cochlear Outer Hair Cells: Localization of an Inositol Triphosphate-Gated Ca2+ Store to the Base of the Sensory Hair Bundle J. Neurosci., August 15, 1999; 19(16): 6918 - 6929. [Abstract] [Full Text] [PDF]

				V. M. Sandler and J.-G. Barbara Calcium-Induced Calcium Release Contributes to Action Potential-Evoked Calcium Transients in Hippocampal CA1 Pyramidal Neurons J. Neurosci., June 1, 1999; 19(11): 4325 - 4336. [Abstract] [Full Text] [PDF]

				P. Sah and J. D. Clements Photolytic Manipulation of [Ca2+]i Reveals Slow Kinetics of Potassium Channels Underlying the Afterhyperpolarization in Hipppocampal Pyramidal Neurons J. Neurosci., May 15, 1999; 19(10): 3657 - 3664. [Abstract] [Full Text] [PDF]

				S. C. Molitor and P. B. Manis Voltage-Gated Ca2+ Conductances in Acutely Isolated Guinea Pig Dorsal Cochlear Nucleus Neurons J Neurophysiol, March 1, 1999; 81(3): 985 - 998. [Abstract] [Full Text] [PDF]

				S. Tekkok, I. Medina, and K. Krnjevic Intraneuronal [Ca2+] Changes Induced by 2-Deoxy-D-Glucose in Rat Hippocampal Slices J Neurophysiol, January 1, 1999; 81(1): 174 - 183. [Abstract] [Full Text] [PDF]