HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Guinamard, R Articles by Teulon, J Search for Related Content PubMed PubMed Citation Articles by Guinamard, R Articles by Teulon, J

INSERM U.323, Paris, France.

1. Chloride channels were identified in the basolateral membrane of isolated cortical thick ascending limbs (CTALs) of the mouse nephron by the patch-clamp technique. A channel with a conductance of 45 pS, previously shown to be Cl- selective, was detected in 21% of cell-attached patches when CTAL fragments were pre-incubated with 10 mumol l-4 forskolin for at least 15 min. The same channel was found in only 8.5% of cell-attached patches formed on unstimulated tubules. 2. Another channel with a smaller conductance (7-9 pS) was found in 42.8% of cell-attached patches and 57% of inside-out patches in unstimulated CTAL tubules, but in 82-87% of patches from forskolin-treated tubules. 3. The small channels was Cl- selective (Cl(-)-to-Na+ permeability ratio, PCl/PNa = 9.8) with the permeability sequence: NO3- > Br- > Cl- > F- > gluconate. Channel activity decreased (Br-) or disappeared (NO3-) at negative voltages. At 140 mmol l-1, I- completely inhibited channel activity at all voltages, but a PI/PCl ratio of 1.6 was estimated using a low I- concentration (10 mmol l-1). 4. Internal adenosine triphosphate (ATP) increased normalized current (nPo) in 48% of inside-out patches containing Cl- channels from unstimulated tubules and in 63% of patches from forskolin-treated CTAL tubules. The non-hydrolysable ATP analogue, adenosine 5'-adenylyl imidodiphosphate (AMP-PNP) did not increase channel activity. 5. Adding the catalytic subunit of protein kinase A to the bath in the presence of ATP increased the activity of the small channel in 58% of inside-out patches from unstimulated tubules, but it had no effect on the 45 pS channel. 6. The Cl- channel blockers 5-nitro-2-(3-phenylpropylamine)-benzoic acid (NPPB), 4,4'-diisothiocyanatostilbene-2,2'-disulphonic acid (DIDS) or glibenclamide, all at 0.1 mmol l-1, and diphenylamine-2-carboxylic acid (DPC), at 1 mmol l-1, inhibited the small channel activity by 80-100% in inside-out patches. 7. These results indicate that two Cl- channels with contrasting properties mediate the basolateral step of NaCl absorption in the thick ascending limb of the loop of Henle.

This article has been cited by other articles:

				A. Nissant, M. Paulais, S. Lachheb, S. Lourdel, and J. Teulon Similar chloride channels in the connecting tubule and cortical collecting duct of the mouse kidney Am J Physiol Renal Physiol, June 1, 2006; 290(6): F1421 - F1429. [Abstract] [Full Text] [PDF]

				A. Nissant, S. Lourdel, S. Baillet, M. Paulais, P. Marvao, J. Teulon, and M. Imbert-Teboul Heterogeneous distribution of chloride channels along the distal convoluted tubule probed by single-cell RT-PCR and patch clamp Am J Physiol Renal Physiol, December 1, 2004; 287(6): F1233 - F1243. [Abstract] [Full Text] [PDF]

				S. Lourdel, M. Paulais, P. Marvao, A. Nissant, and J. Teulon A Chloride Channel at the Basolateral Membrane of the Distal-convoluted Tubule: a Candidate ClC-K Channel J. Gen. Physiol., March 31, 2003; 121(4): 287 - 300. [Abstract] [Full Text] [PDF]

				O. Devuyst and W. B. Guggino Chloride channels in the kidney: lessons learned from knockout animals Am J Physiol Renal Physiol, December 1, 2002; 283(6): F1176 - F1191. [Abstract] [Full Text] [PDF]

				R.-M. Gu and W.-H. Wang Arachidonic acid inhibits K channels in basolateral membrane of the thick ascending limb Am J Physiol Renal Physiol, September 1, 2002; 283(3): F407 - F414. [Abstract] [Full Text] [PDF]

				E. Feraille and A. Doucet Sodium-Potassium-Adenosinetriphosphatase-Dependent Sodium Transport in the Kidney: Hormonal Control Physiol Rev, January 1, 2001; 81(1): 345 - 418. [Abstract] [Full Text] [PDF]

				F. Leviel, D. Eladari, A. Blanchard, J.-S. Poumarat, M. Paillard, and R.-A. Podevin Pathways for HCO-3 exit across the basolateral membrane in rat thick limbs Am J Physiol Renal Physiol, June 1, 1999; 276(6): F847 - F856. [Abstract] [Full Text] [PDF]

				I. Rubera, M. Tauc, M. Bidet, C. Poujeol, B. Cuiller, A. Watrin, N. Touret, and P. Poujeol Chloride currents in primary cultures of rabbit proximal and distal convoluted tubules Am J Physiol Renal Physiol, November 1, 1998; 275(5): F651 - F663. [Abstract] [Full Text] [PDF]

				M. A. Laamarti, P. D. Bell, and J.-Y. Lapointe Transport and regulatory properties of the apical Na-K-2Cl cotransporter of macula densa cells Am J Physiol Renal Physiol, November 1, 1998; 275(5): F703 - F709. [Abstract] [Full Text] [PDF]

				M. A. Bailey and S. J. Walter Renal Effects of Glibenclamide: A Micropuncture Study J. Pharmacol. Exp. Ther., May 1, 1998; 285(2): 464 - 467. [Abstract] [Full Text]

				S. Waldegger and T. J. Jentsch Functional and Structural Analysis of ClC-K Chloride Channels Involved in Renal Disease J. Biol. Chem., August 4, 2000; 275(32): 24527 - 24533. [Abstract] [Full Text] [PDF]

				M. Paulais, S. Lourdel, and J. Teulon Properties of an inwardly rectifying K+ channel in the basolateral membrane of mouse TAL Am J Physiol Renal Physiol, May 1, 2002; 282(5): F866 - F876. [Abstract] [Full Text] [PDF]