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J Physiol Vol 487, Issue Pt 2 pp 283-289
Copyright © 1995 by The Physiological Society
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Phosphorylation of caldesmon by mitogen-activated protein kinase with no effect on Ca2+ sensitivity in rabbit smooth muscle.

G F Nixon, K Iizuka, C M Haystead, T A Haystead, A P Somlyo and A V Somlyo

Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville 22908, USA.

1. Recombinant, activated mitogen-activated protein kinase (3.3 microM; p42mapk) phosphorylated caldesmon in phasic (rabbit portal vein) and tonic (rabbit femoral artery) smooth muscle strips permeabilized with Triton X-100. 2. Phosphorylation of caldesmon by p42mapk neither induced contraction of relaxed smooth muscle nor affected the Ca2+ sensitivity of submaximally contracted permeabilized phasic or tonic smooth muscle.




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