HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Fritsch, J Articles by Edelman, A Search for Related Content PubMed PubMed Citation Articles by Fritsch, J Articles by Edelman, A

CNRS URA 583, Hôpital des Enfants Malades, Paris, France.

1. Hyperpolarization-activated Cl- currents (ICl,hyp) were investigated in the T84 human adenocarcinoma cell line, using the patch-clamp whole-cell configuration. 2. During whole-cell recording with high-chloride and ATP-containing internal solutions, hyperpolarizing jumps from a holding potential of 0 mV elicited slow inward current relaxations, carried by Cl- and detected at membrane potentials more negative than -40 mV. Analysis of the relative permeabilities to monovalent anions gave the following sequence: Cl- > Br- > I- > glutamate. 3. ICl,hyp was partially inhibited by 1 mM diphenylamine-2-carboxylic acid or 0.1 mM 5-nitro-2-(3-phenylpropylamino)-benzoate, and was completely blocked by Cd2+ (> 300 microM). It was insensitive to 1 mM external 4,4'-diisothiocyanatostilbene-2,2'-disulphonic acid or 1 mM Ba2+. 4. ICl,hyp was inhibited by external application of 500 microM cptcAMP (8-(4-chlorophenylthio)-adenosine 3':5'-cyclic monophosphate) or 500 nM of the protein kinase C activator, phorbol 12-myristate, 13-acetate. 5. (i) Omission of ATP from the pipette solution, (ii) ATP replacement by the non-hydrolysable ATP analogue 5'-adenylylimidodiphosphate, and (iii) inhibition of protein kinase C by staurosporine or calphostin C accelerated the activation kinetics of the current and increased its amplitude, but did not alter its pharmacological properties. 6. We conclude that hyperpolarization-activated Cl- channels similar to those of ClC-2 channels (mammalian homologue of Torpedo chloride channel ClC-0) are present in T84 cells, and that their gating properties are modulated by phosphorylation.

This article has been cited by other articles:

				A. Hinzpeter, J. Lipecka, F. Brouillard, M. Baudoin-Legros, M. Dadlez, A. Edelman, and J. Fritsch Association between Hsp90 and the ClC-2 chloride channel upregulates channel function Am J Physiol Cell Physiol, January 1, 2006; 290(1): C45 - C56. [Abstract] [Full Text] [PDF]

				S. U. Dhani, R. Mohammad-Panah, N. Ahmed, C. Ackerley, M. Ramjeesingh, and C. E. Bear Evidence for a Functional Interaction between the ClC-2 Chloride Channel and the Retrograde Motor Dynein Complex J. Biol. Chem., April 25, 2003; 278(18): 16262 - 16270. [Abstract] [Full Text] [PDF]

				M. Catalan, I. Cornejo, C. D. Figueroa, M. I. Niemeyer, F. V. Sepulveda, and L. P. Cid ClC-2 in guinea pig colon: mRNA, immunolabeling, and functional evidence for surface epithelium localization Am J Physiol Gastrointest Liver Physiol, October 1, 2002; 283(4): G1004 - G1013. [Abstract] [Full Text] [PDF]

				K. Nehrke, J. Arreola, H.-V. Nguyen, J. Pilato, L. Richardson, G. Okunade, R. Baggs, G. E. Shull, and J. E. Melvin Loss of Hyperpolarization-activated Cl- Current in Salivary Acinar Cells from Clcn2 Knockout Mice J. Biol. Chem., June 21, 2002; 277(26): 23604 - 23611. [Abstract] [Full Text] [PDF]