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Department of Molecular Physiology, University of Virginia Health Sciences Center, Charlottesville 22908, USA.

1. The apparent second-order rate constants (k+T) of ATP-induced cross-bridge detachment from rigor in the absence of Ca2+ were determined with laser flash photolysis of caged ATP (cATP) in alpha-toxin-permeabilized tonic, rabbit femoral artery and phasic, rabbit bladder smooth muscles. The potential effect of cATP binding to actomyosin (AM) on cross-bridge kinetics was examined by varying the initial concentration of cATP 2-fold. For a given [ATP] released from either 10 or 5 mM cATP, the kinetics of relaxation were not significantly different; the estimated dissociation constant for cATP binding to smooth muscle AM was 1-3 mM. 2. k+T was significantly higher ((9.5 +/- 1.3) x 10(4) M-1 s-1) in the phasic than in the tonic ((3.0 +/- 1.0) x 10(4) M-1 s-1) smooth muscle. 3. We conclude that the combination of the significantly lower (approximately 3 times) apparent second-order rate constant of MgATP association with the approximately 5 times higher affinity of cross-bridges for MgADP in tonic, than in phasic, smooth muscle is a major determinant of the slower kinetics of relaxation and, probably, shortening velocity of tonic smooth muscle.

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				K.-E. Andersson and A. Arner Urinary Bladder Contraction and Relaxation: Physiology and Pathophysiology Physiol Rev, July 1, 2004; 84(3): 935 - 986. [Abstract] [Full Text] [PDF]