HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kentish, J. C. Articles by Wrzosek, A. Search for Related Content PubMed PubMed Citation Articles by Kentish, J. C. Articles by Wrzosek, A.

Changes in force and cytosolic Ca²⁺ concentration after length changes in isolated rat ventricular trabeculae

Jonathan C. Kentish and Antoni Wrzosek

1. Changes in cytosolic [Ca²⁺] ([Ca²⁺]_i) were measured in isolated rat trabeculae that had been micro-injected with fura-2 salt, in order to investigate the mechanism by which twitch force changes following an alteration of muscle length.

2. A step increase in length of the muscle produced a rapid potentiation of twitch force but not of the Ca²⁺ transient. The rapid rise of force was unaffected by inhibiting the sarcoplasmic reticulum (SR) with ryanodine and cyclopiazonic acid.

3. The force-[Ca²⁺]_i relationship of the myofibrils in situ, determined from twitches and tetanic contractions in SR-inhibited muscles, showed that the rapid rise of force was due primarily to an increase in myofibrillar Ca²⁺ sensitivity, with a contribution from an increase in the maximum force production of the myofibrils.

4. After stretch of the muscle there was a further, slow increase of twitch force which was due entirely to a slow increase of the Ca²⁺ transient, since there was no change in the myofibrillar force-[Ca²⁺]_i relationship. SR inhibition slowed down, but did not alter the magnitude of, the slow force response.

5. During the slow rise of force there was no slow increase of diastolic [Ca²⁺]_i, whether or not the SR was inhibited. The same was true in unstimulated muscles.

6. We conclude that the rapid increase in twitch force after muscle stretch is due to the length- dependent properties of the myofibrils. The slow force increase is not explained by length dependence of the myofibrils or the SR, or by a rise in diastolic [Ca²⁺]_i. Evidence from tetani suggests the slow force responses result from increased Ca²⁺ loading of the cell during the action potential.

This article has been cited by other articles:

				J. J. Rice, F. Wang, D. M. Bers, and P. P. de Tombe Approximate Model of Cooperative Activation and Crossbridge Cycling in Cardiac Muscle Using Ordinary Differential Equations Biophys. J., September 1, 2008; 95(5): 2368 - 2390. [Abstract] [Full Text] [PDF]

				J. Kockskamper, M. Khafaga, M. Grimm, A. Elgner, S. Walther, A. Kockskamper, D. von Lewinski, H. Post, M. Grossmann, H. Dorge, et al. Angiotensin II and myosin light-chain phosphorylation contribute to the stretch-induced slow force response in human atrial myocardium Cardiovasc Res, September 1, 2008; 79(4): 642 - 651. [Abstract] [Full Text] [PDF]

				C. G. dos Remedios The Regulation of Muscle Contraction: As in Life, It Keeps Getting More Complex Biophys. J., December 15, 2007; 93(12): 4097 - 4098. [Full Text] [PDF]

				C. I. Caldiz, C. D. Garciarena, R. A. Dulce, L. P. Novaretto, A. M. Yeves, I. L. Ennis, H. E. Cingolani, G. Chiappe de Cingolani, and N. G. Perez Mitochondrial reactive oxygen species activate the slow force response to stretch in feline myocardium J. Physiol., November 1, 2007; 584(3): 895 - 905. [Abstract] [Full Text] [PDF]

				P. Diolez, V. Deschodt-Arsac, G. Raffard, C. Simon, P. D. Santos, E. Thiaudiere, L. Arsac, and J.-M. Franconi Modular regulation analysis of heart contraction: application to in situ demonstration of a direct mitochondrial activation by calcium in beating heart Am J Physiol Regulatory Integrative Comp Physiol, July 1, 2007; 293(1): R13 - R19. [Abstract] [Full Text] [PDF]

				S. A. Niederer and N. P. Smith A Mathematical Model of the Slow Force Response to Stretch in Rat Ventricular Myocytes Biophys. J., June 1, 2007; 92(11): 4030 - 4044. [Abstract] [Full Text] [PDF]

				H. E. Cingolani and I. L. Ennis Sodium-Hydrogen Exchanger, Cardiac Overload, and Myocardial Hypertrophy Circulation, March 6, 2007; 115(9): 1090 - 1100. [Full Text] [PDF]

				R. E. Petre, M. P. Quaile, E. I. Rossman, S. J. Ratcliffe, B. A. Bailey, S. R. Houser, and K. B. Margulies Sex-based differences in myocardial contractile reserve Am J Physiol Regulatory Integrative Comp Physiol, February 1, 2007; 292(2): R810 - R818. [Abstract] [Full Text] [PDF]

				Y. YANIV, C. LEVY, and A. LANDESBERG The Mechanoelectric Feedback: A Novel "Calcium Clamp" Method, Using Tetanic Contraction, for Testing the Role of the Intracellular Free Calcium Ann. N.Y. Acad. Sci., October 1, 2006; 1080(1): 235 - 247. [Abstract] [Full Text] [PDF]

				M. J LAB Mechanosensitive-Mediated Interaction, Integration, and Cardiac Control Ann. N.Y. Acad. Sci., October 1, 2006; 1080(1): 282 - 300. [Abstract] [Full Text] [PDF]

				H. Endo, M. Miura, M. Hirose, J. Takahashi, M. Nakano, Y. Wakayama, Y. Sugai, Y. Kagaya, J. Watanabe, K. Shirato, et al. Reduced Inotropic Effect of Nifekalant in Failing Hearts in Rats J. Pharmacol. Exp. Ther., September 1, 2006; 318(3): 1102 - 1107. [Abstract] [Full Text] [PDF]

				S. A. Niederer, P. J. Hunter, and N. P. Smith A Quantitative Analysis of Cardiac Myocyte Relaxation: A Simulation Study Biophys. J., March 1, 2006; 90(5): 1697 - 1722. [Abstract] [Full Text] [PDF]

				V. Saks, P. Dzeja, U. Schlattner, M. Vendelin, A. Terzic, and T. Wallimann Cardiac system bioenergetics: metabolic basis of the Frank-Starling law J. Physiol., March 1, 2006; 571(2): 253 - 273. [Abstract] [Full Text] [PDF]

				C. LEVY, H. E.D.J. TER KEURS, Y. YANIV, and A. LANDESBERG The Sarcomeric Control of Energy Conversion Ann. N.Y. Acad. Sci., June 1, 2005; 1047(1): 219 - 231. [Abstract] [Full Text] [PDF]

				I. L. Ennis, C. D. Garciarena, N. G. Perez, R. A. Dulce, M. C. Camilion de Hurtado, and H. E. Cingolani Endothelin isoforms and the response to myocardial stretch Am J Physiol Heart Circ Physiol, June 1, 2005; 288(6): H2925 - H2930. [Abstract] [Full Text] [PDF]

				S. Calaghan and E. White Activation of Na+-H+ exchange and stretch-activated channels underlies the slow inotropic response to stretch in myocytes and muscle from the rat heart J. Physiol., August 15, 2004; 559(1): 205 - 214. [Abstract] [Full Text] [PDF]

				M.E Diaz, H.K Graham, and A.W Trafford Enhanced sarcolemmal Ca2+ efflux reduces sarcoplasmic reticulum Ca2+ content and systolic Ca2+ in cardiac hypertrophy Cardiovasc Res, June 1, 2004; 62(3): 538 - 547. [Abstract] [Full Text] [PDF]

				R. L. Moss, M. Razumova, and D. P. Fitzsimons Myosin Crossbridge Activation of Cardiac Thin Filaments: Implications for Myocardial Function in Health and Disease Circ. Res., May 28, 2004; 94(10): 1290 - 1300. [Abstract] [Full Text] [PDF]

				D. von Lewinski, B. Stumme, F. Fialka, C. Luers, and B. Pieske Functional Relevance of the Stretch-Dependent Slow Force Response in Failing Human Myocardium Circ. Res., May 28, 2004; 94(10): 1392 - 1398. [Abstract] [Full Text] [PDF]

				M. Reyes, G. L. Freeman, D. Escobedo, S. Lee, M. E. Steinhelper, and M. D. Feldman Enhancement of Contractility With Sustained Afterload in the Intact Murine Heart: Blunting of Length-Dependent Activation Circulation, June 17, 2003; 107(23): 2962 - 2968. [Abstract] [Full Text] [PDF]

				V. Saks, A. Kuznetsov, T. Andrienko, Y. Usson, F. Appaix, K. Guerrero, T. Kaambre, P. Sikk, M. Lemba, and M. Vendelin Heterogeneity of ADP Diffusion and Regulation of Respiration in Cardiac Cells Biophys. J., May 1, 2003; 84(5): 3436 - 3456. [Abstract] [Full Text] [PDF]

				S. S. Rhodes, K. M. Ropella, S. H. Audi, A. K. S. Camara, L. G. Kevin, P. S. Pagel, and D. F. Stowe Cross-bridge kinetics modeled from myoplasmic [Ca2+] and LV pressure at 17{degrees}C and after 37{degrees}C and 17{degrees}C ischemia Am J Physiol Heart Circ Physiol, April 1, 2003; 284(4): H1217 - H1229. [Abstract] [Full Text] [PDF]

				H. E Cingolani, N. G Perez, B. Pieske, D. von Lewinski, and M. C Camilion de Hurtado Stretch-elicited Na+/H+ exchanger activation: the autocrine/paracrine loop and its mechanical counterpart Cardiovasc Res, March 15, 2003; 57(4): 953 - 960. [Abstract] [Full Text] [PDF]

				D. von Lewinski, B. Stumme, L. S Maier, C. Luers, D. M Bers, and B. Pieske Stretch-dependent slow force response in isolated rabbit myocardium is Na+ dependent Cardiovasc Res, March 15, 2003; 57(4): 1052 - 1061. [Abstract] [Full Text] [PDF]

				J. Piuhola, I. Szokodi, P. Kinnunen, M. Ilves, R. deChatel, O. Vuolteenaho, and H. Ruskoaho Endothelin-1 Contributes to the Frank-Starling Response in Hypertrophic Rat Hearts Hypertension, January 1, 2003; 41(1): 93 - 98. [Abstract] [Full Text] [PDF]

				R. R. Lamberts, M. H. P. van Rijen, P. Sipkema, P. Fransen, S. U. Sys, and N. Westerhof Coronary perfusion and muscle lengthening increase cardiac contraction: different stretch-triggered mechanisms Am J Physiol Heart Circ Physiol, October 1, 2002; 283(4): H1515 - H1522. [Abstract] [Full Text] [PDF]

				R. M. Kanashiro, E. Nozawa, N. Murad, L. R. Gerola, V. A. Moises, and P. J.F. Tucci Myocardial infarction scar plication in the rat: cardiac mechanics in an animal model for surgical procedures Ann. Thorac. Surg., May 1, 2002; 73(5): 1507 - 1513. [Abstract] [Full Text] [PDF]

				J. Shimizu, K. Todaka, and D. Burkhoff Load dependence of ventricular performance explained by model of calcium-myofilament interactions Am J Physiol Heart Circ Physiol, March 1, 2002; 282(3): H1081 - H1091. [Abstract] [Full Text] [PDF]

				Y. Wakayama, M. Miura, Y. Sugai, Y. Kagaya, J. Watanabe, H. E. D. J. ter Keurs, and K. Shirato Stretch and quick release of rat cardiac trabeculae accelerates Ca2+ waves and triggered propagated contractions Am J Physiol Heart Circ Physiol, November 1, 2001; 281(5): H2133 - H2142. [Abstract] [Full Text] [PDF]

				P. J. F. Tucci, N. Murad, C. L. Rossi, R. J. Nogueira, and O. Santana Jr. Heart rate modulates the slow enhancement of contraction due to sudden left ventricular dilation Am J Physiol Heart Circ Physiol, May 1, 2001; 280(5): H2136 - H2143. [Abstract] [Full Text] [PDF]

				H. E. Cingolani, N. G. Perez, and M. C. Camilion de Hurtado An Autocrine/Paracrine Mechanism Triggered by Myocardial Stretch Induces Changes in Contractility Physiology, April 1, 2001; 16(2): 88 - 91. [Abstract] [Full Text] [PDF]

				N. G. Perez, M. C. C. de Hurtado, and H. E. Cingolani Reverse Mode of the Na+-Ca2+ Exchange After Myocardial Stretch : Underlying Mechanism of the Slow Force Response Circ. Res., March 2, 2001; 88(4): 376 - 382. [Abstract] [Full Text] [PDF]

				T. C. Irving, J. Konhilas, D. Perry, R. Fischetti, and P. P. de Tombe Myofilament lattice spacing as a function of sarcomere length in isolated rat myocardium Am J Physiol Heart Circ Physiol, November 1, 2000; 279(5): H2568 - H2573. [Abstract] [Full Text] [PDF]

				T. Ishikawa, H. Kajiwara, and S. Kurihara Alterations in contractile properties and Ca2+ handling in streptozotocin-induced diabetic rat myocardium Am J Physiol Heart Circ Physiol, December 1, 1999; 277(6): H2185 - H2194. [Abstract] [Full Text] [PDF]

				J. C. Kentish A Role for the Sarcolemmal Na+/H+ Exchanger in the Slow Force Response to Myocardial Stretch Circ. Res., October 15, 1999; 85(8): 658 - 660. [Full Text] [PDF]

				B. V. Alvarez, N. G. Perez, I. L. Ennis, M. C. Camilion de Hurtado, and H. E. Cingolani Mechanisms Underlying the Increase in Force and Ca2+ Transient That Follow Stretch of Cardiac Muscle : A Possible Explanation of the Anrep Effect Circ. Res., October 15, 1999; 85(8): 716 - 722. [Abstract] [Full Text] [PDF]

				J. Layland and J. C. Kentish Positive force- and [Ca2+]i-frequency relationships in rat ventricular trabeculae at physiological frequencies Am J Physiol Heart Circ Physiol, January 1, 1999; 276(1): H9 - H18. [Abstract] [Full Text] [PDF]

				T. Ishikawa, H. Kajiwara, and S. Kurihara Modulation of Ca2+ transient decay by tension and Ca2+ removal in hyperthyroid myocardium Am J Physiol Heart Circ Physiol, January 1, 1999; 276(1): H289 - H299. [Abstract] [Full Text] [PDF]