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J Physiol Volume 507, Number 1, 93-103, February 15, 1998
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The Journal of Physiology (1998), 507.1, pp. 93-103
© Copyright 1998 The Physiological Society

Facilitation by the beta2a subunit of pore openings in cardiac Ca2+ channels

James Costantin *, Francesca Noceti *, Ning Qin *, the late Xiangyang Wei, Lutz Birnbaumer *¹ and Enrico Stefani *²³

Departments of * Anesthesiology, ² Physiology and ¹ Biological Chemistry, UCLA School of Medicine, UCLA, Los Angeles, CA 90095, USA and ³ Conicet, Buenos Aires, Argentina

  1. Single channel recordings were performed on the cardiac calcium channel (alpha1C) in order to study the effect of coexpression of the accessory beta2a subunit. On-cell patch clamp recordings were performed after expression of these channels in Xenopus oocytes.

  2. The alpha1C subunit, when expressed alone, had similar single channel properties to native cardiac channels. Slow transitions between low and high open probability (Po) gating modes were found as well as fast gating transitions between the open and closed states.

  3. Coexpression of the beta2a subunit caused changes in the fast gating during high Po mode. In this mode, open time distributions reveal at least three open states and the beta2a subunit favours the occupancy of the longest, 10-15 ms open state. No effect of the beta2a subunit was found when the channel was gating in the low Po mode.

  4. Slow gating transitions were also affected by the beta2a subunit. The high Po mode was maintained for the duration of the depolarizing pulse in the presence of the beta2a subunit; while the alpha1C channel when expressed alone, frequently switched into and out of the high Po mode during the course of a sweep.

  5. The beta2a subunit also affected mode switching that occurred between sweeps. Runs analysis revealed that the alpha1C subunit has a tendency toward non-random mode switching. The beta2a subunit increased this tendency. A chi2 analysis of contingency tables indicated that the beta2a subunit caused the alpha1C channel to gain 'intrinsic memory', meaning that the mode of a given sweep can be non-independent of the mode of the previous sweep.

  6. We conclude that the beta2a subunit causes changes to the alpha1C channel in both its fast and slow gating behaviour. The beta2a subunit alters fast gating by facilitating movement of the channel into an existing open state. Additionally, the beta2a subunit decreases the slow switching between low and high Po modes.




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