HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Knot, H. J. Articles by Nelson, M. T. Search for Related Content PubMed PubMed Citation Articles by Knot, H. J. Articles by Nelson, M. T.

Regulation of arterial diameter and wall [Ca²⁺] in cerebral arteries of rat by membrane potential and intravascular pressure

Harm J. Knot and Mark T. Nelson

1. The regulation of intracellular [Ca²⁺] in the smooth muscle cells in the wall of small pressurized cerebral arteries (100-200 µm) of rat was studied using simultaneous digital fluorescence video imaging of arterial diameter and wall [Ca²⁺], combined with microelectrode measurements of arterial membrane potential.

2. Elevation of intravascular pressure (from 10 to 100 mmHg) caused a membrane depolarization from -63 ± 1 to -36 ± 2 mV, increased arterial wall [Ca²⁺] from 119 ± 10 to 245 ± 9 nM, and constricted the arteries from 208 ± 10 µm (fully dilated, Ca²⁺ free) to 116 ± 7 µm or by 45 % ('myogenic tone').

3. Pressure-induced increases in arterial wall [Ca²⁺] and vasoconstriction were blocked by inhibitors of voltage-dependent Ca²⁺ channels (diltiazem and nisoldipine) or to the same extent by removal of external Ca²⁺.

4. At a steady pressure (i.e. under isobaric conditions at 60 mmHg), the membrane potential was stable at -45 ± 1 mV, intracellular [Ca²⁺] was 190 ± 10 nM, and arteries were constricted by 41 % (to 115 ± 7 µm from 196 ± 8 µm fully dilated). Under this condition of -45 ± 5 mV at 60 mmHg, the voltage sensitivity of wall [Ca²⁺] and diameter were 7·5 nM mV^-1 and 7·5 µm mV^-1, respectively, resulting in a Ca²⁺ sensitivity of diameter of 1 µm nM^-1.

5. Membrane potential depolarization from -58 to -23 mV caused pressurized arteries (to 60 mmHg) to constrict over their entire working range, i.e. from maximally dilated to constricted. This depolarization was associated with an elevation of arterial wall [Ca²⁺] from 124 ± 7 to 347 ± 12 nM. These increases in arterial wall [Ca²⁺] and vasoconstriction were blocked by L-type voltage-dependent Ca²⁺ channel inhibitors.

6. The relationship between arterial wall [Ca²⁺] and membrane potential was not significantly different under isobaric (60 mmHg) and non-isobaric conditions (10-100 mmHg), suggesting that intravascular pressure regulates arterial wall [Ca²⁺] through changes in membrane potential.

7. The results are consistent with the idea that intravascular pressure causes membrane potential depolarization, which opens voltage-dependent Ca²⁺ channels, acting as 'voltage sensors', thus increasing Ca²⁺ entry and arterial wall [Ca²⁺], which leads to vasoconstriction.

This article has been cited by other articles:

				T. E. Link, K. Murakami, M. Beem-Miller, B. I. Tranmer, and G. C. Wellman Oxyhemoglobin-Induced Expression of R-Type Ca2+ Channels in Cerebral Arteries Stroke, July 1, 2008; 39(7): 2122 - 2128. [Abstract] [Full Text] [PDF]

				T. Vaithianathan, A. Bukiya, J. Liu, P. Liu, M. Asuncion-Chin, Z. Fan, and A. Dopico Direct Regulation of BK Channels by Phosphatidylinositol 4,5-Bisphosphate as a Novel Signaling Pathway J. Gen. Physiol., June 30, 2008; 132(1): 13 - 28. [Abstract] [Full Text] [PDF]

				Q. Xi, A. Adebiyi, G. Zhao, K. E. Chapman, C. M. Waters, A. Hassid, and J. H. Jaggar IP3 Constricts Cerebral Arteries via IP3 Receptor-Mediated TRPC3 Channel Activation and Independently of Sarcoplasmic Reticulum Ca2+ Release Circ. Res., May 9, 2008; 102(9): 1118 - 1126. [Abstract] [Full Text] [PDF]

				H. Raina, S. R. Ella, and M. A. Hill Decreased activity of the smooth muscle Na+/Ca2+ exchanger impairs arteriolar myogenic reactivity J. Physiol., March 15, 2008; 586(6): 1669 - 1681. [Abstract] [Full Text] [PDF]

				P. D. Smith, S. E. Brett, K. D. Luykenaar, S. L. Sandow, S. P. Marrelli, E. J. Vigmond, and D. G. Welsh KIR channels function as electrical amplifiers in rat vascular smooth muscle J. Physiol., February 15, 2008; 586(4): 1147 - 1160. [Abstract] [Full Text] [PDF]

				R. Schubert, D. Lidington, and S.-S. Bolz The emerging role of Ca2+ sensitivity regulation in promoting myogenic vasoconstriction Cardiovasc Res, January 1, 2008; 77(1): 8 - 18. [Abstract] [Full Text] [PDF]

				V. Telezhkin, T. Goecks, A. D. Bonev, G. Osol, and N. I. Gokina Decreased function of voltage-gated potassium channels contributes to augmented myogenic tone of uterine arteries in late pregnancy Am J Physiol Heart Circ Physiol, January 1, 2008; 294(1): H272 - H284. [Abstract] [Full Text] [PDF]

				H. A. Coleman and H. C. Parkington Endothelin enhances activity of mechanosensitive channels: A mechanism for ET augmentation of the myogenic response Cardiovasc Res, November 1, 2007; 76(2): 197 - 198. [Full Text] [PDF]

				M. F. Navedo, G. C. Amberg, R. E. Westenbroek, M. J. Sinnegger-Brauns, W. A. Catterall, J. Striessnig, and L. F. Santana Cav1.3 channels produce persistent calcium sparklets, but Cav1.2 channels are responsible for sparklets in mouse arterial smooth muscle Am J Physiol Heart Circ Physiol, September 1, 2007; 293(3): H1359 - H1370. [Abstract] [Full Text] [PDF]

				A. N. Bukiya, J. Liu, L. Toro, and A. M. Dopico beta1 (KCNMB1) Subunits Mediate Lithocholate Activation of Large-Conductance Ca2+-Activated K+ Channels and Dilation in Small, Resistance-Size Arteries Mol. Pharmacol., August 1, 2007; 72(2): 359 - 369. [Abstract] [Full Text] [PDF]

				S. A. Reading and J. E. Brayden Central Role of TRPM4 Channels in Cerebral Blood Flow Regulation Stroke, August 1, 2007; 38(8): 2322 - 2328. [Abstract] [Full Text] [PDF]

				K. D. Luykenaar and D. G. Welsh Activators of the PKA and PKG pathways attenuate RhoA-mediated suppression of the KDR current in cerebral arteries Am J Physiol Heart Circ Physiol, June 1, 2007; 292(6): H2654 - H2663. [Abstract] [Full Text] [PDF]

				C. A. Cobine, B. P. Callaghan, and K. D. Keef Role of L-type calcium channels and PKC in active tone development in rabbit coronary artery Am J Physiol Heart Circ Physiol, June 1, 2007; 292(6): H3079 - H3088. [Abstract] [Full Text] [PDF]

				S. Earley, S. V. Straub, and J. E. Brayden Protein kinase C regulates vascular myogenic tone through activation of TRPM4 Am J Physiol Heart Circ Physiol, June 1, 2007; 292(6): H2613 - H2622. [Abstract] [Full Text] [PDF]

				M. A. Hill and M. J. Davis Coupling a change in intraluminal pressure to vascular smooth muscle depolarization: still stretching for an explanation Am J Physiol Heart Circ Physiol, June 1, 2007; 292(6): H2570 - H2572. [Full Text] [PDF]

				A. Adebiyi, G. Zhao, S. Y. Cheranov, A. Ahmed, and J. H. Jaggar Caveolin-1 abolishment attenuates the myogenic response in murine cerebral arteries Am J Physiol Heart Circ Physiol, March 1, 2007; 292(3): H1584 - H1592. [Abstract] [Full Text] [PDF]

				J. Zacharia, J. Zhang, and W. G. Wier Ca2+ signaling in mouse mesenteric small arteries: myogenic tone and adrenergic vasoconstriction Am J Physiol Heart Circ Physiol, March 1, 2007; 292(3): H1523 - H1532. [Abstract] [Full Text] [PDF]

				G. C. Amberg, M. F. Navedo, M. Nieves-Cintron, J. D. Molkentin, and L. F. Santana Calcium sparklets regulate local and global calcium in murine arterial smooth muscle J. Physiol., February 15, 2007; 579(1): 187 - 201. [Abstract] [Full Text] [PDF]

				B. P. Ander, C. Hurtado, C. S. Raposo, T. G. Maddaford, J. F. Deniset, L. V. Hryshko, G. N. Pierce, and A. Lukas Differential sensitivities of the NCX1.1 and NCX1.3 isoforms of the Na+-Ca2+ exchanger to {alpha}-linolenic acid Cardiovasc Res, January 15, 2007; 73(2): 395 - 403. [Abstract] [Full Text] [PDF]

				I. Ito, Y. P. R. Jarajapu, M. B Grant, and H. J Knot Characteristics of myogenic tone in the rat ophthalmic artery Am J Physiol Heart Circ Physiol, January 1, 2007; 292(1): H360 - H368. [Abstract] [Full Text] [PDF]

				N. L. Jernigan and H. A. Drummond Myogenic vasoconstriction in mouse renal interlobar arteries: role of endogenous beta and {gamma}ENaC Am J Physiol Renal Physiol, December 1, 2006; 291(6): F1184 - F1191. [Abstract] [Full Text] [PDF]

				H. Xu, W. F. Jackson, G. D. Fink, and J. J. Galligan Activation of Potassium Channels by Tempol in Arterial Smooth Muscle Cells From Normotensive and Deoxycorticosterone Acetate-Salt Hypertensive Rats Hypertension, December 1, 2006; 48(6): 1080 - 1087. [Abstract] [Full Text] [PDF]

				M. A. Machingal and S. V. Ramanan A Steady-State Electrochemical Model of Vascular Smooth Muscle Cells Biophys. J., September 1, 2006; 91(5): 1648 - 1662. [Abstract] [Full Text] [PDF]

				A. Li, A. Adebiyi, C. W. Leffler, and J. H. Jaggar KCa channel insensitivity to Ca2+ sparks underlies fractional uncoupling in newborn cerebral artery smooth muscle cells Am J Physiol Heart Circ Physiol, September 1, 2006; 291(3): H1118 - H1125. [Abstract] [Full Text] [PDF]

				G. C. Amberg and L. F. Santana Kv2 channels oppose myogenic constriction of rat cerebral arteries Am J Physiol Cell Physiol, August 1, 2006; 291(2): C348 - C356. [Abstract] [Full Text] [PDF]

				S. V. Straub and M. T. Nelson Molecular Coding of Kv1 Channels to Oppose Myogenic Constriction Circ. Res., July 7, 2006; 99(1): 13 - 14. [Full Text] [PDF]

				T. T. Chen, K. D. Luykenaar, E. J. Walsh, M. P. Walsh, and W. C. Cole Key Role of Kv1 Channels in Vasoregulation Circ. Res., July 7, 2006; 99(1): 53 - 60. [Abstract] [Full Text] [PDF]

				S. Earley and M. T. Nelson Central role of Ca2+-dependent regulation of vascular tone in vivo J Appl Physiol, July 1, 2006; 101(1): 10 - 11. [Full Text] [PDF]

				D. Xiao, J. N. Buchholz, and L. Zhang Pregnancy attenuates uterine artery pressure-dependent vascular tone: role of PKC/ERK pathway Am J Physiol Heart Circ Physiol, June 1, 2006; 290(6): H2337 - H2343. [Abstract] [Full Text] [PDF]

				X. Cheng and J. H. Jaggar Genetic ablation of caveolin-1 modifies Ca2+ spark coupling in murine arterial smooth muscle cells Am J Physiol Heart Circ Physiol, June 1, 2006; 290(6): H2309 - H2319. [Abstract] [Full Text] [PDF]

				M. F. Navedo, G. C. Amberg, M. Nieves, J. D. Molkentin, and L. F. Santana Mechanisms Underlying Heterogeneous Ca2+ Sparklet Activity in Arterial Smooth Muscle J. Gen. Physiol., May 30, 2006; 127(6): 611 - 622. [Abstract] [Full Text] [PDF]

				N. I. Gokina and T. Goecks Upregulation of endothelial cell Ca2+ signaling contributes to pregnancy-enhanced vasodilation of rat uteroplacental arteries Am J Physiol Heart Circ Physiol, May 1, 2006; 290(5): H2124 - H2135. [Abstract] [Full Text] [PDF]

				B. R. Wamhoff, D. K. Bowles, and G. K. Owens Excitation-Transcription Coupling in Arterial Smooth Muscle Circ. Res., April 14, 2006; 98(7): 868 - 878. [Abstract] [Full Text] [PDF]

				J. Ledoux, M. E. Werner, J. E. Brayden, and M. T. Nelson Calcium-Activated Potassium Channels and the Regulation of Vascular Tone Physiology, February 1, 2006; 21(1): 69 - 78. [Abstract] [Full Text] [PDF]

				J. S. Naik, L. Xiang, and R. L. Hester Enhanced role for RhoA-associated kinase in adrenergic-mediated vasoconstriction in gracilis arteries from obese Zucker rats Am J Physiol Regulatory Integrative Comp Physiol, January 1, 2006; 290(1): R154 - R161. [Abstract] [Full Text] [PDF]

				J. Zhang, M. Y. Lee, M. Cavalli, L. Chen, R. Berra-Romani, C. W. Balke, G. Bianchi, P. Ferrari, J. M. Hamlyn, T. Iwamoto, et al. Sodium pump {alpha}2 subunits control myogenic tone and blood pressure in mice J. Physiol., November 15, 2005; 569(1): 243 - 256. [Abstract] [Full Text] [PDF]

				Y. P. R. Jarajapu and H. J. Knot Relative contribution of Rho kinase and protein kinase C to myogenic tone in rat cerebral arteries in hypertension Am J Physiol Heart Circ Physiol, November 1, 2005; 289(5): H1917 - H1922. [Abstract] [Full Text] [PDF]

				H. Xu, X. Bian, S. W. Watts, and A. Hlavacova Activation of Vascular BK Channel by Tempol in DOCA-Salt Hypertensive Rats Hypertension, November 1, 2005; 46(5): 1154 - 1162. [Abstract] [Full Text] [PDF]

				K. A. Dora Does arterial myogenic tone determine blood flow distribution in vivo? Am J Physiol Heart Circ Physiol, October 1, 2005; 289(4): H1323 - H1325. [Full Text] [PDF]

				M.-J. Xie, L.-F. Zhang, J. Ma, and H.-W. Cheng Functional alterations in cerebrovascular K+ and Ca2+ channels are comparable between simulated microgravity rat and SHR Am J Physiol Heart Circ Physiol, September 1, 2005; 289(3): H1265 - H1276. [Abstract] [Full Text] [PDF]

				M. F. Navedo, G. C. Amberg, V. S. Votaw, and L. F. Santana Constitutively active L-type Ca2+ channels PNAS, August 2, 2005; 102(31): 11112 - 11117. [Abstract] [Full Text] [PDF]

				N. I. Gokina, K. M. Park, K. McElroy-Yaggy, and G. Osol Effects of Rho kinase inhibition on cerebral artery myogenic tone and reactivity J Appl Physiol, May 1, 2005; 98(5): 1940 - 1948. [Abstract] [Full Text] [PDF]

				S. A. Reading, S. Earley, B. J. Waldron, D. G. Welsh, and J. E. Brayden TRPC3 mediates pyrimidine receptor-induced depolarization of cerebral arteries Am J Physiol Heart Circ Physiol, May 1, 2005; 288(5): H2055 - H2061. [Abstract] [Full Text] [PDF]

				H. J. Knot, I. Laher, E. A. Sobie, S. Guatimosim, L. Gomez-Viquez, H. Hartmann, L.-S. Song, W.J. Lederer, W. F. Graier, R. Malli, et al. Twenty Years of Calcium Imaging: Cell Physiology to Dye For Mol. Interv., April 1, 2005; 5(2): 112 - 127. [Abstract] [Full Text] [PDF]

				M. Ishiguro, T. L. Wellman, A. Honda, S. R. Russell, B. I. Tranmer, and G. C. Wellman Emergence of a R-Type Ca2+ Channel (CaV 2.3) Contributes to Cerebral Artery Constriction After Subarachnoid Hemorrhage Circ. Res., March 4, 2005; 96(4): 419 - 426. [Abstract] [Full Text] [PDF]

				J. S. Naik, S. Earley, T. C. Resta, and B. R. Walker Pressure-induced smooth muscle cell depolarization in pulmonary arteries from control and chronically hypoxic rats does not cause myogenic vasoconstriction J Appl Physiol, March 1, 2005; 98(3): 1119 - 1124. [Abstract] [Full Text] [PDF]

				C. L. Heaps, D. L. Tharp, and D. K. Bowles Hypercholesterolemia abolishes voltage-dependent K+ channel contribution to adenosine-mediated relaxation in porcine coronary arterioles Am J Physiol Heart Circ Physiol, February 1, 2005; 288(2): H568 - H576. [Abstract] [Full Text] [PDF]

				P. Liu, Q. Xi, A. Ahmed, J. H. Jaggar, and A. M. Dopico Essential role for smooth muscle BK channels in alcohol-induced cerebrovascular constriction PNAS, December 28, 2004; 101(52): 18217 - 18222. [Abstract] [Full Text] [PDF]

				N. L. Jernigan, B. R. Walker, and T. C. Resta Chronic hypoxia augments protein kinase G-mediated Ca2+ desensitization in pulmonary vascular smooth muscle through inhibition of RhoA/Rho kinase signaling Am J Physiol Lung Cell Mol Physiol, December 1, 2004; 287(6): L1220 - L1229. [Abstract] [Full Text] [PDF]

				S. Earley, T. C. Resta, and B. R. Walker Disruption of smooth muscle gap junctions attenuates myogenic vasoconstriction of mesenteric resistance arteries Am J Physiol Heart Circ Physiol, December 1, 2004; 287(6): H2677 - H2686. [Abstract] [Full Text] [PDF]

				R. S. Scotland, S. Chauhan, C. Davis, C. De Felipe, S. Hunt, J. Kabir, P. Kotsonis, U. Oh, and A. Ahluwalia Vanilloid Receptor TRPV1, Sensory C-Fibers, and Vascular Autoregulation: A Novel Mechanism Involved in Myogenic Constriction Circ. Res., November 12, 2004; 95(10): 1027 - 1034. [Abstract] [Full Text] [PDF]

				G. C. Amberg, C. F. Rossow, M. F. Navedo, and L. F. Santana NFATc3 Regulates Kv2.1 Expression in Arterial Smooth Muscle J. Biol. Chem., November 5, 2004; 279(45): 47326 - 47334. [Abstract] [Full Text] [PDF]

				A. Ahmed, C. M. Waters, C. W. Leffler, and J. H. Jaggar Ionic mechanisms mediating the myogenic response in newborn porcine cerebral arteries Am J Physiol Heart Circ Physiol, November 1, 2004; 287(5): H2061 - H2069. [Abstract] [Full Text] [PDF]

				S. Earley, B. J. Waldron, and J. E. Brayden Critical Role for Transient Receptor Potential Channel TRPM4 in Myogenic Constriction of Cerebral Arteries Circ. Res., October 29, 2004; 95(9): 922 - 929. [Abstract] [Full Text] [PDF]

				N. L. Jernigan, B. R. Walker, and T. C. Resta Endothelium-derived reactive oxygen species and endothelin-1 attenuate NO-dependent pulmonary vasodilation following chronic hypoxia Am J Physiol Lung Cell Mol Physiol, October 1, 2004; 287(4): L801 - L808. [Abstract] [Full Text] [PDF]

				Z.-J. Fu, M.-J. Xie, L.-F. Zhang, H.-W. Cheng, and J. Ma Differential activation of potassium channels in cerebral and hindquarter arteries of rats during simulated microgravity Am J Physiol Heart Circ Physiol, October 1, 2004; 287(4): H1505 - H1515. [Abstract] [Full Text] [PDF]

				S. Verlohren, G. Dubrovska, S.-Y. Tsang, K. Essin, F. C. Luft, Y. Huang, and M. Gollasch Visceral Periadventitial Adipose Tissue Regulates Arterial Tone of Mesenteric Arteries Hypertension, September 1, 2004; 44(3): 271 - 276. [Abstract] [Full Text] [PDF]

				J. J. Hamann, J. B. Buckwalter, and P. S. Clifford Vasodilatation is obligatory for contraction-induced hyperaemia in canine skeletal muscle J. Physiol., June 15, 2004; 557(3): 1013 - 1020. [Abstract] [Full Text] [PDF]

				C. Guibert, R. Marthan, and J.-P. Savineau 5-HT induces an arachidonic acid-sensitive calcium influx in rat small intrapulmonary artery Am J Physiol Lung Cell Mol Physiol, June 1, 2004; 286(6): L1228 - L1236. [Abstract] [Full Text] [PDF]

				G. Isenberg Ca2+ Control of Transcription: Can We Extrapolate Signaling Cascades From Neurons to Vascular Smooth Muscle Cells? Circ. Res., May 28, 2004; 94(10): 1276 - 1278. [Full Text] [PDF]

L. V. Gonzalez Bosc, M. K. Wilkerson, K. N. Bradley, D. M. Eckman, D. C. Hill-Eubanks, and M. T. Nelson Intraluminal Pressure Is a Stimulus for NFATc3 Nuclear Accumulation: ROLE OF CALCIUM, ENDOTHELIUM-DERIVED NITRIC OXIDE, AND cGMP-DEPENDENT PROTEIN KINASE J. Biol. Chem., March 12, 2004; 279(11): 10702 - 1