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Muscarinic facilitation of GABA release in substantia gelatinosa of the rat spinal dorsal horn

H. Baba, T. Kohno, M. Okamoto, P. A. Goldstein *, K. Shimoji and M. Yoshimura ¹

1. Blind patch clamp recordings were made from substantia gelatinosa (SG) neurones in the adult rat spinal cord slice to study the mechanisms of cholinergic modulation of GABAergic inhibition.

2. In the majority of SG neurones tested, carbachol (10 µM) increased the frequency (677 % of control) of spontaneous GABAergic inhibitory postsynaptic currents (IPSCs). A portion of these events appeared to result from the generation of spikes by GABAergic interneurones, since large amplitude IPSCs were eliminated by tetrodotoxin (1 µM).

3. The effect of carbachol on spontaneous IPSCs was mimicked by neostigmine, suggesting that GABAergic interneurones are under tonic regulation by cholinergic systems.

4. The frequency of GABAergic miniature IPSCs in the presence of tetrodotoxin (1 µM) was also increased by carbachol without affecting amplitude distribution, indicating that acetylcholine facilitates quantal release of GABA through presynaptic mechanisms.

5. Neither the M₁ receptor agonist McN-A-343 (10-300 µM) nor the M₂ receptor agonist, arecaidine (10-100 µM), mimicked the effects of carbachol. All effects of carbachol and neostigmine were antagonized by atropine (1 µM), while pirenzepine (100 nM), methoctramine (1 µM) and hexahydrosiladifenidol hydrochloride, p-fluoro-analog (100 nM) had no effect.

6. Focal stimulation of deep dorsal horn, but not dorsolateral funiculus, evoked a similar increase in IPSC frequency to that evoked by carbachol and neostigmine. The stimulation-induced facilitation of GABAergic transmission lasted for 2-3 min post stimulation, and the effect was antagonized by atropine (100 nM).

7. Our observations suggest that GABAergic interneurones possess muscarinic receptors on both axon terminals and somatodendritic sites, that the activation of these receptors increases the excitability of inhibitory interneurones and enhances GABA release in SG and that the GABAergic inhibitory system is further controlled by cholinergic neurones located in the deep dorsal horn. Those effects may be responsible for the antinociceptive action produced by the intrathecal administration of muscarinic agonists and acetylcholinesterase inhibitors.

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