HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Andrade, F. H. Articles by Westerblad, H. Search for Related Content PubMed PubMed Citation Articles by Andrade, F. H. Articles by Westerblad, H.

Effect of nitric oxide on single skeletal muscle fibres from the mouse

Francisco H. Andrade *¹, Michael B. Reid ², David G. Allen ³ and Håkan Westerblad *

1. Single skeletal muscle fibres from a mouse foot muscle were used to investigate the effects of nitric oxide on contractile function.

2. We measured force production and myoplasmic free Ca²⁺ concentration ([Ca²⁺]_i) in single fibres exposed to the nitric oxide donors S-nitroso-N-acetylcysteine (SNAC) and nitroprusside.

3. The nitric oxide donors reduced myofibrillar Ca²⁺ sensitivity, whereas [Ca²⁺]_i transients were increased during submaximal tetani. Force was largely unchanged. SNAC did not change maximum shortening velocity, the rate of force redevelopment, or force production at saturating [Ca²⁺]_i.

4. The guanylyl cyclase inhibitor LY83583 increased tetanic [Ca²⁺]_i but had no effect on Ca²⁺ sensitivity. LY83583 did not prevent the decrease in myofibrillar Ca²⁺ sensitivity in response to SNAC. The oxidizer sodium nitrite increased tetanic [Ca²⁺]_i and decreased myofibrillar Ca²⁺ sensitivity.

5. We conclude that under our experimental conditions nitric oxide impairs Ca²⁺ activation of the actin filaments which results in decreased myofibrillar Ca²⁺ sensitivity.

This article has been cited by other articles:

				E. Prochniewicz, D. A. Lowe, D. J. Spakowicz, L. Higgins, K. O'Conor, L. V. Thompson, D. A. Ferrington, and D. D. Thomas Functional, structural, and chemical changes in myosin associated with hydrogen peroxide treatment of skeletal muscle fibers Am J Physiol Cell Physiol, February 1, 2008; 294(2): C613 - C626. [Abstract] [Full Text] [PDF]

				J. N. Edwards, W. A. Macdonald, C. van der Poel, and D. G. Stephenson O2bullet production at 37{degrees}C plays a critical role in depressing tetanic force of isolated rat and mouse skeletal muscle Am J Physiol Cell Physiol, August 1, 2007; 293(2): C650 - C660. [Abstract] [Full Text] [PDF]

				D. J. Baker, D. J. Krause, R. A. Howlett, and R. T. Hepple Nitric oxide synthase inhibition reduces O2 cost of force development and spares high-energy phosphates following contractions in pump-perfused rat hindlimb muscles Exp Physiol, May 1, 2006; 91(3): 581 - 589. [Abstract] [Full Text] [PDF]

				C. A. C. Ottenheijm, L. M. A. Heunks, M. C. P. Geraedts, and P. N. R. Dekhuijzen Hypoxia-induced skeletal muscle fiber dysfunction: role for reactive nitrogen species Am J Physiol Lung Cell Mol Physiol, January 1, 2006; 290(1): L127 - L135. [Abstract] [Full Text] [PDF]

				J. L. Betters, D. S. Criswell, R. A. Shanely, D. Van Gammeren, D. Falk, K. C. DeRuisseau, M. Deering, T. Yimlamai, and S. K. Powers Trolox Attenuates Mechanical Ventilation-induced Diaphragmatic Dysfunction and Proteolysis Am. J. Respir. Crit. Care Med., December 1, 2004; 170(11): 1179 - 1184. [Abstract] [Full Text] [PDF]

				S. Pouvreau, B. Allard, C. Berthier, and V. Jacquemond Control of intracellular calcium in the presence of nitric oxide donors in isolated skeletal muscle fibres from mouse J. Physiol., November 1, 2004; 560(3): 779 - 794. [Abstract] [Full Text] [PDF]

				J. P. Eu, J. M. Hare, D. T. Hess, M. Skaf, J. Sun, I. Cardenas-Navina, Q.-A. Sun, M. Dewhirst, G. Meissner, and J. S. Stamler Concerted regulation of skeletal muscle contractility by oxygen tension and endogenous nitric oxide PNAS, December 9, 2003; 100(25): 15229 - 15234. [Abstract] [Full Text] [PDF]

				X. Zhu, L. M. A. Heunks, H. A. Machiels, L. Ennen, and P. N. R. Dekhuijzen Effects of modulation of nitric oxide on rat diaphragm isotonic contractility during hypoxia J Appl Physiol, February 1, 2003; 94(2): 612 - 620. [Abstract] [Full Text] [PDF]

				M. B. Reid, J. Lannergren, and H. Westerblad Respiratory and Limb Muscle Weakness Induced by Tumor Necrosis Factor-{alpha}: Involvement of Muscle Myofilaments Am. J. Respir. Crit. Care Med., August 15, 2002; 166(4): 479 - 484. [Abstract] [Full Text] [PDF]

				L. M. A. Heunks, H. A. Machiels, P. N. R. Dekhuijzen, Y. S. Prakash, and G. C. Sieck Nitric oxide affects sarcoplasmic calcium release in skeletal myotubes J Appl Physiol, November 1, 2001; 91(5): 2117 - 2124. [Abstract] [Full Text] [PDF]

				L. M. A. Heunks, M. J. Cody, P. C. Geiger, P. N. R. Dekhuijzen, and G. C. Sieck Nitric oxide impairs Ca2+ activation and slows cross-bridge cycling kinetics in skeletal muscle J Appl Physiol, November 1, 2001; 91(5): 2233 - 2239. [Abstract] [Full Text] [PDF]

				D. E. Baranano and S. H. Snyder Neural roles for heme oxygenase: Contrasts to nitric oxide synthase PNAS, September 25, 2001; 98(20): 10996 - 11002. [Abstract] [Full Text] [PDF]

				C. R. RICHMONDS and H. J. KAMINSKI Nitric oxide synthase expression and effects of nitric oxide modulation on contractility of rat extraocular muscle FASEB J, August 1, 2001; 15(10): 1764 - 1770. [Abstract] [Full Text] [PDF]

				A. S. COMTOIS, E. BARREIRO, P. L. HUANG, A. MARETTE, M. PERRAULT, and S. N. A. HUSSAIN Lipopolysaccharide-induced Diaphragmatic Contractile Dysfunction and Sarcolemmal Injury in Mice Lacking the Neuronal Nitric Oxide Synthase Am. J. Respir. Crit. Care Med., March 15, 2001; 163(4): 977 - 982. [Abstract] [Full Text]

				M. B. Reid Plasticity in Skeletal, Cardiac, and Smooth Muscle: Invited Review: Redox modulation of skeletal muscle contraction: what we know and what we don't J Appl Physiol, February 1, 2001; 90(2): 724 - 731. [Abstract] [Full Text] [PDF]

				L. A. Callahan, D. Nethery, D. Stofan, A. DiMarco, and G. Supinski Free Radical-Induced Contractile Protein Dysfunction in Endotoxin-Induced Sepsis Am. J. Respir. Cell Mol. Biol., February 1, 2001; 24(2): 210 - 217. [Abstract] [Full Text]

				L. A. Callahan, Z. W. She, and T. M. Nosek Superoxide, hydroxyl radical, and hydrogen peroxide effects on single-diaphragm fiber contractile apparatus J Appl Physiol, January 1, 2001; 90(1): 45 - 54. [Abstract] [Full Text] [PDF]

				J. S. Stamler and G. Meissner Physiology of Nitric Oxide in Skeletal Muscle Physiol Rev, January 1, 2001; 81(1): 209 - 237. [Abstract] [Full Text] [PDF]

				L. M A Heunks and P N R. Dekhuijzen Respiratory muscle function and free radicals: from cell to COPD Thorax, August 1, 2000; 55(8): 704 - 716. [Full Text]

				S. S. Segal, S. E. Brett, and W. C. Sessa Codistribution of NOS and caveolin throughout peripheral vasculature and skeletal muscle of hamsters Am J Physiol Heart Circ Physiol, September 1, 1999; 277(3): H1167 - H1177. [Abstract] [Full Text] [PDF]

				D. E. Rassier, B. R. MacIntosh, and W. Herzog Length dependence of active force production in skeletal muscle J Appl Physiol, May 1, 1999; 86(5): 1445 - 1457. [Abstract] [Full Text] [PDF]

				H. Drexler Endothelium as a Therapeutic Target in Heart Failure Circulation, December 15, 1998; 98(24): 2652 - 2655. [Full Text] [PDF]