J Physiol Society Membership
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Physiol Volume 509, Number 3, 651-665, June 15, 1998
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Yu, C. R.
Right arrow Articles by Role, L. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yu, C. R.
Right arrow Articles by Role, L. W.
The Journal of Physiology (1998), 509.3, pp. 651-665
© Copyright 1998 The Physiological Society

Functional contribution of the alpha7 subunit to multiple subtypes of nicotinic receptors in embryonic chick sympathetic neurones

C. Ron Yu and Lorna W. Role

Department of Anatomy and Cell Biology in the Center for Neurobiology and Behavior, College of Physicians and Surgeons, Columbia University, 722 West 168th Street, Research Annex Room 807, New York, NY 10032, USA

  1. Many studies of the alpha7 subunit of the neuronal nicotinic acetylcholine receptor (nAChR) family have demonstrated that this alpha-bungarotoxin (alpha-BgTx)-binding neuronal receptor can participate in ACh-gated channels. Heterologous expression studies reveal that alpha7 subunits form homomeric channels of unusually high Ca2+ permeability. However, the physiological role of the alpha7 subunit in native neuronal nAChR channels is less clear.

  2. We present evidence that the alpha7 subunit contributes to the function of at least three subtypes of native nAChR expressed by embryonic chick sympathetic neurones. These subtypes are functionally distinct from heterologously expressed homomeric alpha7 nAChRs as well as homomeric-like currents described in studies of hippocampal and parasympathetic neurones.

  3. The proposed nAChRs differ from one another and from homomeric alpha7 nAChRs in their sensitivity to block by alpha7 subunit-specific antagonists: alpha-BgTx and methyllycaconitine (MLA). While MLA blocks 60 % of the macroscopic ACh response, alpha-BgTx inhibits a small component of the macroscopic current described by slow-on and slow-off kinetics.

  4. Functional deletion of the alpha7 subunit by antisense oligonucleotide treatment eliminates the susceptibility of the nAChRs to block by both MLA and alpha-BgTx.

  5. Single channel recordings combined with pharmacological and antisense-mediated 'deletion' techniques reveal that alpha-BgTx-sensitive alpha7-containing nAChRs have a small unitary conductance (18 pS), brief open time kinetics and relatively low open probability (Po). MLA-sensitive alpha7 nAChRs are characterized by a conductance of ~35 pS, intermediate burst duration, and a relatively high Po.

  6. The third nAChR subtype deleted by alpha7 antisense treatment is characterized by a unitary conductance of 50 pS and prolonged opening duration.

  7. We propose that these three populations of native alpha7-containing nAChRs are distinct heteromeric complexes that include other alpha and/or beta nAChR subunits.




This article has been cited by other articles:


Home page
 Hum Mol GenetHome page
S. V. Mathew, A. J. Law, B. K. Lipska, M. I. Davila-Garcia, E. D. Zamora, S. N. Mitkus, R. Vakkalanka, R. E. Straub, D. R. Weinberger, J. E. Kleinman, et al.
{alpha}7 nicotinic acetylcholine receptor mRNA expression and binding in postmortem human brain are associated with genetic variation in neuregulin 1
Hum. Mol. Genet., December 1, 2007; 16(23): 2921 - 2932.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
D. Fayuk and J. L Yakel
Ca2+ permeability of nicotinic acetylcholine receptors in rat hippocampal CA1 interneurones
J. Physiol., August 1, 2005; 566(3): 759 - 768.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
C. Gotti, M. Moretti, F. Clementi, L. Riganti, J. M. McIntosh, A. C. Collins, M. J. Marks, and P. Whiteaker
Expression of Nigrostriatal {alpha}6-Containing Nicotinic Acetylcholine Receptors Is Selectively Reduced, but Not Eliminated, by {beta}3 Subunit Gene Deletion
Mol. Pharmacol., June 1, 2005; 67(6): 2007 - 2015.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
H. Fischer, A. Orr-Urtreger, L. W Role, and S. Huck
Selective deletion of the {alpha}5 subunit differentially affects somatic-dendritic versus axonally targeted nicotinic ACh receptors in mouse
J. Physiol., February 15, 2005; 563(1): 119 - 137.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
E. G. Severance, H. Zhang, Y. Cruz, S. Pakhlevaniants, S. H. Hadley, J. Amin, L. Wecker, C. Reed, and J. Cuevas
The {alpha}7 Nicotinic Acetylcholine Receptor Subunit Exists in Two Isoforms that Contribute to Functional Ligand-Gated Ion Channels
Mol. Pharmacol., September 1, 2004; 66(3): 420 - 429.
[Abstract] [Full Text] [PDF]

Home page
 Learn. Mem.Home page
V. I. Pidoplichko, J. Noguchi, O. O. Areola, Y. Liang, J. Peterson, T. Zhang, and J. A. Dani
Nicotinic Cholinergic Synaptic Mechanisms in the Ventral Tegmental Area Contribute to Nicotine Addiction
Learn. Mem., January 1, 2004; 11(1): 60 - 69.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
X. W. Fu, C. A. Nurse, S. M. Farragher, and E. Cutz
Expression of functional nicotinic acetylcholine receptors in neuroepithelial bodies of neonatal hamster lung
Am J Physiol Lung Cell Mol Physiol, December 1, 2003; 285(6): L1203 - L1212.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
L. Zhao, Y.-P. Kuo, A. A. George, J.-H. Peng, M. S. Purandare, K. M. Schroeder, R. J. Lukas, and J. Wu
Functional Properties of Homomeric, Human {alpha}7-Nicotinic Acetylcholine Receptors Heterologously Expressed in the SH-EP1 Human Epithelial Cell Line
J. Pharmacol. Exp. Ther., June 1, 2003; 305(3): 1132 - 1141.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
R. Salas, A. Orr-Urtreger, R. S. Broide, A. Beaudet, R. Paylor, and M. De Biasi
The Nicotinic Acetylcholine Receptor Subunit alpha 5 Mediates Short-Term Effects of Nicotine in Vivo
Mol. Pharmacol., May 1, 2003; 63(5): 1059 - 1066.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
J. R. A. Wooltorton, V. I. Pidoplichko, R. S. Broide, and J. A. Dani
Differential Desensitization and Distribution of Nicotinic Acetylcholine Receptor Subtypes in Midbrain Dopamine Areas
J. Neurosci., April 15, 2003; 23(8): 3176 - 3185.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
J.-H. Son and S. Meizel
Evidence Suggesting That the Mouse Sperm Acrosome Reaction Initiated by the Zona Pellucida Involves an {alpha}7 Nicotinic Acetylcholine Receptor
Biol Reprod, April 1, 2003; 68(4): 1348 - 1353.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
W. G. Conroy, Q.-S. Liu, Q. Nai, J. F. Margiotta, and D. K. Berg
Potentiation of alpha 7-Containing Nicotinic Acetylcholine Receptors by Select Albumins
Mol. Pharmacol., February 1, 2003; 63(2): 419 - 428.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
D. Eddins, A. D. Sproul, L. K. Lyford, J. T. McLaughlin, and R. L. Rosenberg
Glutamate 172, essential for modulation of L247T alpha 7 ACh receptors by Ca2+, lines the extracellular vestibule
Am J Physiol Cell Physiol, November 1, 2002; 283(5): C1454 - C1460.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
E. Christophe, A. Roebuck, J. F. Staiger, D. J. Lavery, S. Charpak, and E. Audinat
Two Types of Nicotinic Receptors Mediate an Excitation of Neocortical Layer I Interneurons
J Neurophysiol, September 1, 2002; 88(3): 1318 - 1327.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
C. Bray, J.-H. Son, and S. Meizel
A Nicotinic Acetylcholine Receptor Is Involved in the Acrosome Reaction of Human Sperm Initiated by Recombinant Human ZP3
Biol Reprod, September 1, 2002; 67(3): 782 - 788.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
A. J. Mogg, P. Whiteaker, J. M. McIntosh, M. Marks, A. C. Collins, and S. Wonnacott
Methyllycaconitine Is a Potent Antagonist of alpha -Conotoxin-MII-Sensitive Presynaptic Nicotinic Acetylcholine Receptors in Rat Striatum
J. Pharmacol. Exp. Ther., July 1, 2002; 302(1): 197 - 204.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
M. Dourado and P. B. Sargent
Properties of Nicotinic Receptors Underlying Renshaw Cell Excitation by alpha -Motor Neurons in Neonatal Rat Spinal Cord
J Neurophysiol, June 1, 2002; 87(6): 3117 - 3125.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
S. Ji, T. Tosaka, B. H. Whitfield, A. N. Katchman, A. Kandil, B. C. Knollmann, and S. N. Ebert
Differential Rate Responses to Nicotine in Rat Heart: Evidence for Two Classes of Nicotinic Receptors
J. Pharmacol. Exp. Ther., June 1, 2002; 301(3): 893 - 899.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
S. Fucile, E. Palma, A. Martinez-Torres, R. Miledi, and F. Eusebi
The single-channel properties of human acetylcholine alpha 7 receptors are altered by fusing alpha 7 to the green fluorescent protein
PNAS, March 19, 2002; 99(6): 3956 - 3961.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
K. Fujino and D. Oertel
Cholinergic Modulation of Stellate Cells in the Mammalian Ventral Cochlear Nucleus
J. Neurosci., September 15, 2001; 21(18): 7372 - 7383.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
C. Du and L. W. Role
Differential Modulation of Nicotinic Acetylcholine Receptor Subtypes and Synaptic Transmission in Chick Sympathetic Ganglia by PGE2
J Neurophysiol, June 1, 2001; 85(6): 2498 - 2508.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
M. Ferreira, S. N. Ebert, D. C. Perry, R. P. Yasuda, C. M. Baker, M. I. Dávila-García, K. J. Kellar, and R. A. Gillis
Evidence of a Functional alpha 7-Neuronal Nicotinic Receptor Subtype Located on Motoneurons of the Dorsal Motor Nucleus of the Vagus
J. Pharmacol. Exp. Ther., April 13, 2001; 296(2): 260 - 269.
[Abstract] [Full Text]

Home page
 Proc. Natl. Acad. Sci. USAHome page
Q.-s. Liu, H. Kawai, and D. K. Berg
beta -Amyloid peptide blocks the response of alpha 7-containing nicotinic receptors on hippocampal neurons
PNAS, March 22, 2001; (2001) 81553598.
[Abstract] [Full Text]

Home page
 Proc. Natl. Acad. Sci. USAHome page
G. Sharma and S. Vijayaraghavan
Nicotinic cholinergic signaling in hippocampal astrocytes involves calcium-induced calcium release from intracellular stores
PNAS, March 16, 2001; (2001) 71540198.
[Abstract] [Full Text]

Home page
 J. Neurophysiol.Home page
M. E. McNerney, D. Pardi, P. C. Pugh, Q. Nai, and J. F. Margiotta
Expression and Channel Properties of alpha -Bungarotoxin-Sensitive Acetylcholine Receptors on Chick Ciliary and Choroid Neurons
J Neurophysiol, September 1, 2000; 84(3): 1314 - 1329.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
S. Bibevski, Y. Zhou, J. M. McIntosh, R. E. Zigmond, and M. E. Dunlap
Functional Nicotinic Acetylcholine Receptors That Mediate Ganglionic Transmission in Cardiac Parasympathetic Neurons
J. Neurosci., July 1, 2000; 20(13): 5076 - 5082.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. C. Hogg, L. P. Miranda, D. J. Craik, R. J. Lewis, P. F. Alewood, and D. J. Adams
Single Amino Acid Substitutions in alpha -Conotoxin PnIA Shift Selectivity for Subtypes of the Mammalian Neuronal Nicotinic Acetylcholine Receptor
J. Biol. Chem., December 17, 1999; 274(51): 36559 - 36564.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
L. K. Lyford and R. L. Rosenberg
Cell-free Expression and Functional Reconstitution of Homo-oligomeric alpha 7 Nicotinic Acetylcholine Receptors into Planar Lipid Bilayers
J. Biol. Chem., September 3, 1999; 274(36): 25675 - 25681.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
Q.-S. Liu and D. K. Berg
Extracellular Calcium Regulates Responses of Both alpha 3- and alpha 7-Containing Nicotinic Receptors on Chick Ciliary Ganglion Neurons
J Neurophysiol, September 1, 1999; 82(3): 1124 - 1132.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
L. D. Milner and L. T. Landmesser
Cholinergic and GABAergic Inputs Drive Patterned Spontaneous Motoneuron Activity before Target Contact
J. Neurosci., April 15, 1999; 19(8): 3007 - 3022.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
G. E. Torres, W. R. Haines, T. M. Egan, and M. M. Voigt
Co-Expression of P2X1 and P2X5 Receptor Subunits Reveals a Novel ATP-Gated Ion Channel
Mol. Pharmacol., December 1, 1998; 54(6): 989 - 993.
[Abstract] [Full Text]

Home page
 Proc. Natl. Acad. Sci. USAHome page
G. Sharma and S. Vijayaraghavan
From the Cover: Nicotinic cholinergic signaling in hippocampal astrocytes involves calcium-induced calcium release from intracellular stores
PNAS, March 27, 2001; 98(7): 4148 - 4153.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
Q.-s. Liu, H. Kawai, and D. K. Berg
beta -Amyloid peptide blocks the response of alpha 7-containing nicotinic receptors on hippocampal neurons
PNAS, April 10, 2001; 98(8): 4734 - 4739.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
S. S. Khiroug, P. C. Harkness, P. W. Lamb, S. N. Sudweeks, L. Khiroug, N. S. Millar, and J. L. Yakel
Rat nicotinic ACh receptor alpha7 and beta2 subunits co-assemble to form functional heteromeric nicotinic receptor channels
J. Physiol., April 15, 2002; 540(2): 425 - 434.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1998 The Physiological Society.