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Stealth ryanodine-sensitive Ca²⁺ release contributes to activity of capacitative Ca²⁺ entry and nitric oxide synthase in bovine endothelial cells

Jolanta Paltauf-Doburzynska, Karla Posch, Guenther Paltauf and Wolfgang F. Graier

The involvement of ryanodine-sensitive Ca²⁺ release (RsCR) in bradykinin (Bk)-induced Ca²⁺ release, capacitative Ca²⁺ entry (CCE) and nitric oxide synthase (NOS) activation was assessed in freshly isolated bovine coronary artery endothelial cells.

Using deconvolution microscopy fura-2 was found throughout the whole cytosol, while the cell membrane impermeable dye FFP-18 was exclusively in the cell membrane. Thus, perinuclear ([Ca²⁺]_pn) and subplasmalemmal Ca²⁺ concentration ([Ca²⁺]_sp) were monitored using fura-2 and FFP-18.

Inhibition of Na⁺-Ca²⁺ exchange by lowering extracellular Na⁺ concentration augmented the Bk-induced [Ca²⁺]_pn signal in Ca²⁺-free solution. This effect was abolished when RsCR was prevented with 25 µmol l^-1 ryanodine, while inhibition of RsCR had no effect on Bk-induced increase in [Ca²⁺]_pn without inhibition of Na⁺-Ca²⁺ exchange.

Initiating RsCR by 200 nmol l^-1 ryanodine increased [Ca²⁺]_sp, while [Ca²⁺]_pn remained constant. However, when Na⁺-Ca²⁺ exchange was prevented, ryanodine was also able to elevate [Ca²⁺]_pn.

Blockage of RsCR diminished Ca²⁺ extrusion in response to stimulation with Bk in normal Na⁺-containing solution.

Inhibition of RsCR blunted Bk-activated CCE, while inhibition of Na⁺-Ca²⁺ exchange during stimulation enhanced CCE.

Although direct activation of RsCR failed to activate NOS, inhibition of RsCR diminished the effect of ATP and Bk on NOS, while the effect of thapsigargin remained unchanged.

These data suggest that during stimulation subplasmalemmal RsCR occurs, which contributes to the activities of CCE and NOS. Thus, the function of the subplasmalemmal Ca²⁺ control unit must be extended as a regulator for CCE and NOS.

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