HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Naftalin, R. J. Articles by Pedley, K. C. Search for Related Content PubMed PubMed Citation Articles by Naftalin, R. J. Articles by Pedley, K. C.

Regional crypt function in rat large intestine in relation to fluid absorption and growth of the pericryptal sheath

R. J. Naftalin and K. C. Pedley

Confocal microscopic studies of rat colonic mucosa showed that the pericryptal sheath surrounding distal colonic crypts is an effective barrier both to dextran and NaCl movement, whereas no such structure surrounds the caecal crypts.

The distal colonic pericryptal barrier was functionally demonstrated by accumulation of Sodium Green within the pericryptal space. After exposure to benzamil, Sodium Green accumulation was decreased. Fluorescein isocyanate-labelled dextran (FITC dextran; molecular mass 10000 Da) was accumulated in the crypt lumens and pericryptal spaces. Both dextran and Sodium Green accumulation were absent from the pericryptal zone surrounding caecal crypts.

Low dietary Na⁺ intake raised rat plasma aldosterone and stimulated distal pericryptal sheath growth and adhesiveness as shown by increased amounts of F-actin, smooth muscle actin, -catenin and E-cadherins in the pericryptal zone. It also raised the capacity of the distal colon to dehydrate against a high luminal hydraulic resistance. This linkage indicates that trophic effects on the colon resulting from a low Na⁺ diet are not confined solely to effects on transepithelial Na⁺ transport, but are observed in the pericryptal sheath.

A computer model of crypt function confirms that a pericryptal sheath with low permeability to NaCl is an essential component of the crypt dehydrating mechanism.

This article has been cited by other articles:

				Y. Kikuchi, T. G. Kashima, T. Nishiyama, K. Shimazu, Y. Morishita, M. Shimazaki, I. Kii, H. Horie, H. Nagai, A. Kudo, et al. Periostin Is Expressed in Pericryptal Fibroblasts and Cancer-associated Fibroblasts in the Colon J. Histochem. Cytochem., August 1, 2008; 56(8): 753 - 764. [Abstract] [Full Text] [PDF]

				D. Heitzmann and R. Warth Physiology and Pathophysiology of Potassium Channels in Gastrointestinal Epithelia Physiol Rev, July 1, 2008; 88(3): 1119 - 1182. [Abstract] [Full Text] [PDF]

				E. Cristia, C. Amat, R. J. Naftalin, and M. Moreto Role of vasopressin in rat distal colon function J. Physiol., January 15, 2007; 578(2): 413 - 424. [Abstract] [Full Text] [PDF]

				H Mutoh, S Sakurai, K Satoh, H Osawa, T Tomiyama, H Kita, T Yoshida, K Tamada, H Yamamoto, N Isoda, et al. Pericryptal fibroblast sheath in intestinal metaplasia and gastric carcinoma Gut, January 1, 2005; 54(1): 33 - 39. [Abstract] [Full Text] [PDF]

				R. H. Stephens, J. Tanianis-Hughes, N. B. Higgs, M. Humphrey, and G. Warhurst Region-Dependent Modulation of Intestinal Permeability by Drug Efflux Transporters: In Vitro Studies in mdr1a(-/-) Mouse Intestine J. Pharmacol. Exp. Ther., December 1, 2002; 303(3): 1095 - 1101. [Abstract] [Full Text] [PDF]

				P. Gallardo, N. Olea, and F. V. Sepulveda Distribution of aquaporins in the colon of Octodon degus, a South American desert rodent Am J Physiol Regulatory Integrative Comp Physiol, September 1, 2002; 283(3): R779 - R788. [Abstract] [Full Text] [PDF]

				K. Hirasawa, Y. Sato, Y. Hosoda, T. Yamamoto, and H. Hanai Immunohistochemical Localization of Angiotensin II Receptor and Local Renin-Angiotensin System in Human Colonic Mucosa J. Histochem. Cytochem., February 1, 2002; 50(2): 275 - 282. [Abstract] [Full Text] [PDF]

				K. Kunzelmann and M. Mall Electrolyte Transport in the Mammalian Colon: Mechanisms and Implications for Disease Physiol Rev, January 1, 2002; 82(1): 245 - 289. [Abstract] [Full Text] [PDF]

				J. R. Thiagarajah, S. Jayaraman, R. J. Naftalin, and A. S. Verkman In vivo fluorescence measurement of Na+ concentration in the pericryptal space of mouse descending colon Am J Physiol Cell Physiol, December 1, 2001; 281(6): C1898 - C1903. [Abstract] [Full Text] [PDF]

				J R Thiagarajah, P Gourmelon, N M Griffiths, F Lebrun, R J Naftalin, and K C Pedley Radiation induced cytochrome c release causes loss of rat colonic fluid absorption by damage to crypts and pericryptal myofibroblasts Gut, November 1, 2000; 47(5): 675 - 684. [Abstract] [Full Text] [PDF]

				K. S. Wang, T. Ma, F. Filiz, A. S. Verkman, and J. A. Bastidas Colon water transport in transgenic mice lacking aquaporin-4 water channels Am J Physiol Gastrointest Liver Physiol, August 1, 2000; 279(2): G463 - G470. [Abstract] [Full Text] [PDF]

				G. Egidy, L. Juillerat-Jeanneret, P. Korth, F. T. Bosman, and F. Pinet The endothelin system in normal human colon Am J Physiol Gastrointest Liver Physiol, July 1, 2000; 279(1): G211 - G222. [Abstract] [Full Text] [PDF]

				A T McKie, P S Zammit, and R J Naftalin Comparison of cattle and sheep colonic permeabilities to horseradish peroxidase and hamster scrapie prion protein in vitro Gut, December 1, 1999; 45(6): 879 - 888. [Abstract] [Full Text] [PDF]

				D. W. Powell, R. C. Mifflin, J. D. Valentich, S. E. Crowe, J. I. Saada, and A. B. West Myofibroblasts. II. Intestinal subepithelial myofibroblasts Am J Physiol Cell Physiol, August 1, 1999; 277(2): C183 - C201. [Abstract] [Full Text] [PDF]