J Physiol Society Meetings
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Physiol Volume 515, Number 3, 669-683, March 15, 1999
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Marunaka, Y.
Right arrow Articles by Eaton, D. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Marunaka, Y.
Right arrow Articles by Eaton, D. C.
The Journal of Physiology (1999), 515.3, pp. 669-683
© Copyright 1999 The Physiological Society

Regulation of an amiloride-sensitive Na+-permeable channel by a beta2-adrenergic agonist, cytosolic Ca2+ and Cl- in fetal rat alveolar epithelium

Yoshinori Marunaka, Naomi Niisato, Hugh O'Brodovich and Douglas C. Eaton *

Lung Biology and MRC Group in Lung Development, The Hospital for Sick Children Research Institute, Department of Pediatrics and Institute of Medical Science, The University of Toronto Faculty of Medicine, Toronto, Ontario, Canada M5G 1X8 and * Center for Cell and Molecular Signaling and Department of Physiology, Emory University School of Medicine, Atlanta, GA 30322, USA


In cell-attached patches formed on the apical membrane of fetal alveolar epithelium, terbutaline (a specific beta2-adrenergic agonist) increased the open probability (Po) of an amiloride-sensitive Na+-permeable non-selective cation (NSC) channel (control, 0·03 ± 0·04; terbutaline, 0·62 ± 0·18; n = 8, P < 0·00001) by increasing the mean open time 100-fold without any significant change in the mean closed time and without any change in the single channel conductance (control, 27·8 ± 2·3 pS; terbutaline, 28·2 ± 2·1 pS; n = 8).


The Po of the unstimulated channel increased when the apical membrane was depolarized due to a decrease in the closing rate and an increase in the opening rate, while the Po of the terbutaline-stimulated channel did not depend on the membrane potential.


Increased cytosolic [Ca2+] also increased the Po of the channel in a manner consistent with one Ca2+-binding site on the cytosolic surface of the channel. Terbutaline increased the sensitivity of the channel to cytosolic Ca2+ by shifting the concentration of cytosolic Ca2+ ([Ca2+]c) required for half-maximal activation to a lower [Ca2+]c value, leading to an increase in Po.


An increase in the cytosolic Cl- concentration ([Cl-]c) decreased the Po of the channel consistent with two Cl--binding sites by increasing the closing rate without any significant change in the opening rate. Terbutaline increased Po by reducing the effect of cytosolic Cl- to promote channel closing.


Taken together, these observations indicate that terbutaline activates a Ca2+-activated, Cl--inhibitable, amiloride-sensitive, Na+-permeable NSC channel in fetal rat alveolar epithelium in two ways: first, through an increase in Ca2+ sensitivity, and second, through a reduction in the effect of cytosolic Cl- to promote channel closing.


This article has been cited by other articles:


Home page
 Am. J. Respir. Cell Mol. Bio.Home page
M. Yasuda, N. Niisato, H. Miyazaki, T. Hama, K. Dejima, Y. Hisa, and Y. Marunaka
Epithelial Ion Transport of Human Nasal Polyp and Paranasal Sinus Mucosa
Am. J. Respir. Cell Mol. Biol., April 1, 2007; 36(4): 466 - 472.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
M. N. Helms, J. Self, H. F. Bao, L. C. Job, L. Jain, and D. C. Eaton
Dopamine activates amiloride-sensitive sodium channels in alveolar type I cells in lung slice preparations
Am J Physiol Lung Cell Mol Physiol, October 1, 2006; 291(4): L610 - L618.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
H. G. Folkesson and M. A. Matthay
Alveolar Epithelial Ion and Fluid Transport: Recent Progress
Am. J. Respir. Cell Mol. Biol., July 1, 2006; 35(1): 10 - 19.
[Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
G. M. Mutlu and J. I. Sznajder
Mechanisms of pulmonary edema clearance
Am J Physiol Lung Cell Mol Physiol, November 1, 2005; 289(5): L685 - L695.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
V. Swystun, L. Chen, P. Factor, B. Siroky, P. D. Bell, and S. Matalon
Apical trypsin increases ion transport and resistance by a phospholipase C-dependent rise of Ca2+
Am J Physiol Lung Cell Mol Physiol, May 1, 2005; 288(5): L820 - L830.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
Y. Xie and J. A. Schafer
Inhibition of ENaC by intracellular Cl- in an MDCK clone with high ENaC expression
Am J Physiol Renal Physiol, October 1, 2004; 287(4): F722 - F731.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H.-P. Ma, O. Al-Khalili, S. Ramosevac, S. Saxena, Y.-Y. Liang, D. G. Warnock, and D. C. Eaton
Steroids and Exogenous {gamma}-ENaC Subunit Modulate Cation Channels Formed by {alpha}-ENaC in Human B Lymphocytes
J. Biol. Chem., August 6, 2004; 279(32): 33206 - 33212.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
S. J. Ramminger, K. Richard, S. K. Inglis, S. C. Land, R. E. Olver, and S. M. Wilson
A regulated apical Na+ conductance in dexamethasone-treated H441 airway epithelial cells
Am J Physiol Lung Cell Mol Physiol, August 1, 2004; 287(2): L411 - L419.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
X. Fang, Y. Song, R. Zemans, J. Hirsch, and M. A. Matthay
Fluid transport across cultured rat alveolar epithelial cells: a novel in vitro system
Am J Physiol Lung Cell Mol Physiol, July 1, 2004; 287(1): L104 - L110.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
M. T. Clunes, A. G. Butt, and S. M. Wilson
A glucocorticoid-induced Na+ conductance in human airway epithelial cells identified by perforated patch recording
J. Physiol., June 15, 2004; 557(3): 809 - 819.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
C. Shiima-Kinoshita, K.-Y. Min, T. Hanafusa, H. Mori, and T. Nakahari
{beta}2-adrenergic regulation of ciliary beat frequency in rat bronchiolar epithelium: potentiation by isosmotic cell shrinkage
J. Physiol., January 15, 2004; 554(2): 403 - 416.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
Y. Berthiaume
Long-term stimulation of alveolar epithelial cells by {beta}-adrenergic agonists: increased Na+ transport and modulation of cell growth?
Am J Physiol Lung Cell Mol Physiol, October 1, 2003; 285(4): L798 - L801.
[Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
A. Lazrak and S. Matalon
cAMP-induced changes of apical membrane potentials of confluent H441 monolayers
Am J Physiol Lung Cell Mol Physiol, August 1, 2003; 285(2): L443 - L450.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
S. M. O'Grady and S. Y. Lee
Chloride and potassium channel function in alveolar epithelial cells
Am J Physiol Lung Cell Mol Physiol, May 1, 2003; 284(5): L689 - L700.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Sheng, C. J. Perry, and T. R. Kleyman
External Nickel Inhibits Epithelial Sodium Channel by Binding to Histidine Residues within the Extracellular Domains of alpha and gamma Subunits and Reducing Channel Open Probability
J. Biol. Chem., December 13, 2002; 277(51): 50098 - 50111.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
S. Matalon, A. Lazrak, L. Jain, and D. C. Eaton
Lung Edema Clearance: 20 Years of Progress: Invited Review: Biophysical properties of sodium channels in lung alveolar epithelial cells
J Appl Physiol, November 1, 2002; 93(5): 1852 - 1859.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
R. Olivier, U. Scherrer, J.-D. Horisberger, B. C. Rossier, and E. Hummler
Lung Edema Clearance: 20 Years of Progress: Selected Contribution: Limiting Na+ transport rate in airway epithelia from alpha -ENaC transgenic mice: a model for pulmonary edema
J Appl Physiol, November 1, 2002; 93(5): 1881 - 1887.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
P. M. Barker and R. E. Olver
Lung Edema Clearance: 20 Years of Progress: Invited Review: Clearance of lung liquid during the perinatal period
J Appl Physiol, October 1, 2002; 93(4): 1542 - 1548.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
M. A. Matthay, H. G. Folkesson, and C. Clerici
Lung Epithelial Fluid Transport and the Resolution of Pulmonary Edema
Physiol Rev, July 1, 2002; 82(3): 569 - 600.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
A. Norlin and H. G. Folkesson
Alveolar Epithelial Ion and Fluid Transport: Ca2+-dependent stimulation of alveolar fluid clearance in near-term fetal guinea pigs
Am J Physiol Lung Cell Mol Physiol, April 1, 2002; 282(4): L642 - L649.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
E. D. CRANDALL and M. A. MATTHAY
Alveolar Epithelial Transport . Basic Science to Clinical Medicine
Am. J. Respir. Crit. Care Med., March 15, 2001; 163(4): 1021 - 1029.
[Full Text]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
B. E. Isakson, W. H. Evans, and S. Boitano
Intercellular Ca2+ signaling in alveolar epithelial cells through gap junctions and by extracellular ATP
Am J Physiol Lung Cell Mol Physiol, February 1, 2001; 280(2): L221 - L228.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
S. M. O'Grady, X. Jiang, and D. H. Ingbar
Cl-channel activation is necessary for stimulation of Na transport in adult alveolar epithelial cells
Am J Physiol Lung Cell Mol Physiol, February 1, 2000; 278(2): L239 - L244.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
P. J. Kemp, Z. Borok, K. J. Kim, R. L. Lubman, S. I. Danto, and E. D. Crandall
Epidermal growth factor regulation in adult rat alveolar type II cells of amiloride-sensitive cation channels
Am J Physiol Cell Physiol, December 1, 1999; 277(6): C1058 - C1065.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
N. Niisato, Y. Ito, and Y. Marunaka
cAMP stimulates Na+ transport in rat fetal pneumocyte: involvement of a PTK- but not a PKA-dependent pathway
Am J Physiol Lung Cell Mol Physiol, October 1, 1999; 277(4): L727 - L736.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
I. Lang and H. Martens
Na transport in sheep rumen is modulated by voltage-dependent cation conductance in apical membrane
Am J Physiol Gastrointest Liver Physiol, September 1, 1999; 277(3): G609 - G618.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
A. Collett, S. J. Ramminger, R. E. Olver, and S. M. Wilson
Alveolar Epithelial Ion and Fluid Transport: beta -Adrenoceptor-mediated control of apical membrane conductive properties in fetal distal lung epithelia
Am J Physiol Lung Cell Mol Physiol, April 1, 2002; 282(4): L621 - L630.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
X.-J. Chen, D. C. Eaton, and L. Jain
Alveolar Epithelial Ion and Fluid Transport: beta -Adrenergic regulation of amiloride-sensitive lung sodium channels
Am J Physiol Lung Cell Mol Physiol, April 1, 2002; 282(4): L609 - L620.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1999 The Physiological Society.