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J Physiol Volume 516, Number 2, 377-383, April 15, 1999
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The Journal of Physiology (1999), 516.2, pp. 377-383
© Copyright 1999 The Physiological Society

Isoform-specific regulation of the sodium pump by alpha- and beta-adrenergic agonists in the guinea-pig ventricle

J. Gao, R. Wymore, R. T. Wymore, Y. Wang, D. McKinnon, J. E. Dixon, R. T. Mathias, I. S. Cohen and G. J. Baldo

Department of Physiology & Biophysics and Institute of Molecular Cardiology, State University of New York, Stony Brook, NY 11794-8661, USA


Guinea-pig ventricle was used in the RNase protection assays to determine which alpha-isoforms of the Na+-K+ pumps are present, and ventricular myocytes were used in whole cell patch clamp studies to investigate the actions of alpha- and beta-adrenergic agonists on Na+-K+ pump current.


RNase protection assays showed that two isoforms of the alpha-subunit of the Na+-K+-ATPase are present in guinea-pig ventricle. The mRNA for the alpha1-isoform comprises 82 % of the total pump message, the rest being the alpha2-isoform.


We have previously shown that beta-adrenergic agonists affect Na+-K+ pump current (Ip) through a protein kinase A (PKA)-dependent pathway. We now show that these beta-effects are targeted to the alpha1-isoform of the Na+-K+ pumps.


We have also previously shown that alpha-adrenergic agonists increase Ip through a protein kinase C (PKC)-dependent pathway. We now show that these alpha-isoform effects are targeted to the alpha2-isoform of the Na+-K+ pumps.


These results suggest the effects of adrenergic activation on Na+-K+ pump activity in the heart can be regionally specific, depending on which alpha-isoform of the Na+-K+ pump is expressed.


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