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3 AChRs: impact of 
2, 4 and 5 subunits
We performed single channel analysis on human 3 acetylcholine receptors (AChRs) in Xenopus oocytes and native AChRs from the human neuroblastoma cell line IMR-32. 3 AChRs exhibit channel properties that reflect subunit composition.
32 AChR open times were 0·71 ± 0·14 and 3·5 ± 0·4 ms with a predominant conductance of 26 pS. 34 AChRs had open times of 1·4 ± 0·2 and 6·5 ± 0·8 ms and a predominant conductance of 31 pS. Burst times were 0·82 ± 0·12 and 5·3 ± 0·7 ms for 32 and 1·7 ± 0·1 and 16 ± 1 ms for 34. Desensitization was faster for AChRs with the 2 subunit than for those with the 4 subunit.
One open time for 352 AChRs (5·5 ± 0·3 ms) was different from those of 32 AChRs. For 354 AChRs, an additional conductance, open time and burst time (36 pS, 22 ± 3 ms and 43 ± 4 ms, respectively) were different from those for 34 AChRs.
3 AChRs were inhibited by hexamethonium or mecamylamine. The rate constants for block of 34 by hexamethonium and of 32 by mecamylamine were 1·2 × 107 and 4·6 × 107 M-1 s-1, respectively.
AChRs from IMR-32 cells had a predominant conductance of 32 pS and open times of 1·5 ± 0·3 and 9·6 ± 1·2 ms. These properties were most similar to those of 34 AChRs expressed in oocytes. Antibodies revealed that 5 ± 2 % of IMR-32 3 AChRs contained 5 subunits and 6 ± 2 % contained 2 subunits. IMR-32 3 AChRs are primarily 34 AChRs.
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