| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1C subunit are independent functions of the 
subunit
The subunits of voltage-sensitive calcium channels facilitate the incorporation of channels into the plasma membrane and modulate calcium currents. In order to determine whether these two effects of the subunit are interdependent or independent of each other we studied plasma membrane incorporation of the channel subunits with green fluorescent protein and immunofluorescence labelling, and current modulation with whole-cell and single-channel patch-clamp recordings in transiently transfected human embryonic kidney tsA201 cells.
Coexpression of rabbit cardiac muscle 1C with rabbit skeletal muscle 1a, rabbit heart/brain 2a or rat brain 3 subunits resulted in the colocalization of 1C with and in a marked translocation of the channel complexes into the plasma membrane. In parallel, the whole-cell current density and single-channel open probability were increased. Furthermore, the 2a isoform specifically altered the voltage dependence of current activation and the inactivation kinetics.
A single amino acid substitution in the subunit interaction domain of 1C (1CY467S) disrupted the colocalization and plasma membrane targeting of both subunits without affecting the subunit-induced modulation of whole-cell currents and single-channel properties.
These results show that the modulation of calcium currents by subunits can be explained by subunit-induced changes of single-channel properties, but the formation of stable 1C- complexes and their increased incorporation into the plasma membrane appear not to be necessary for functional modulation.
This article has been cited by other articles:
|
|
 |
|
  Q. Z. Lao, E. Kobrinsky, J. B. Harry, A. Ravindran, and N. M. Soldatov New Determinant for the CaV{beta}2 Subunit Modulation of the CaV1.2 Calcium Channel J. Biol. Chem., June 6, 2008; 283(23): 15577 - 15588. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. D. Schertzer, C. v. d. Poel, T. Shavlakadze, M. D. Grounds, and G. S. Lynch Muscle-specific overexpression of IGF-I improves E-C coupling in skeletal muscle fibers from dystrophic mdx mice Am J Physiol Cell Physiol, January 1, 2008; 294(1): C161 - C168. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Cheng, J. Liu, M. Asuncion-Chin, E. Blaskova, J. P. Bannister, A. M. Dopico, and J. H. Jaggar A Novel CaV1.2 N Terminus Expressed in Smooth Muscle Cells of Resistance Size Arteries Modifies Channel Regulation by Auxiliary Subunits J. Biol. Chem., October 5, 2007; 282(40): 29211 - 29221. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Haase Ahnak, a new player in {beta}-adrenergic regulation of the cardiac L-type Ca2+ channel Cardiovasc Res, January 1, 2007; 73(1): 19 - 25. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. M. Crump, R. N. Correll, E. A. Schroder, W. C. Lester, B. S. Finlin, D. A. Andres, and J. Satin L-type calcium channel {alpha}-subunit and protein kinase inhibitors modulate Rem-mediated regulation of current Am J Physiol Heart Circ Physiol, October 1, 2006; 291(4): H1959 - H1971. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. J. Butcher, J. Leroy, M. W. Richards, W. S. Pratt, and A. C. Dolphin The importance of occupancy rather than affinity of CaV{beta} subunits for the calcium channel I-II linker in relation to calcium channel function J. Physiol., July 15, 2006; 574(2): 387 - 398. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Kanevsky and N. Dascal Regulation of Maximal Open Probability Is a Separable Function of Cav{beta} Subunit in L-type Ca2+ Channel, Dependent on NH2 Terminus of {alpha}1C (Cav1.2{alpha}) J. Gen. Physiol., June 26, 2006; 128(1): 15 - 36. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. C. Garcia, E. Carrillo, J. M. Galindo, A. Hernandez, J. A. Copello, M. Fill, and J. A. Sanchez Short-Term Regulation of Excitation-Contraction Coupling by the {beta}1a Subunit in Adult Mouse Skeletal Muscle Biophys. J., December 1, 2005; 89(6): 3976 - 3984. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Hohaus, S. Beyl, M. Kudrnac, S. Berjukow, E. N. Timin, R. Marksteiner, M. A. Maw, and S. Hering Structural Determinants of L-type Channel Activation in Segment IIS6 Revealed by a Retinal Disorder J. Biol. Chem., November 18, 2005; 280(46): 38471 - 38477. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. X. Takahashi, J. Miriyala, L. H. Tay, D. T. Yue, and H. M. Colecraft A CaV{beta} SH3/Guanylate Kinase Domain Interaction Regulates Multiple Properties of Voltage-gated Ca2+ Channels J. Gen. Physiol., September 26, 2005; 126(4): 365 - 377. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Dalton, S. X Takahashi, J. Miriyala, and H. M Colecraft A single CaV{beta} can reconstitute both trafficking and macroscopic conductance of voltage-dependent calcium channels J. Physiol., September 15, 2005; 567(3): 757 - 769. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. M. Cohen, J. D. Foell, R. C. Balijepalli, V. Shah, J. W. Hell, and T. J. Kamp Unique modulation of L-type Ca2+ channels by short auxiliary {beta}1d subunit present in cardiac muscle Am J Physiol Heart Circ Physiol, May 1, 2005; 288(5): H2363 - H2374. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Berrou, Y. Dodier, A. Raybaud, A. Tousignant, O. Dafi, J. N. Pelletier, and L. Parent The C-terminal Residues in the Alpha-interacting Domain (AID) Helix Anchor CaV{beta} Subunit Interaction and Modulation of CaV2.3 Channels J. Biol. Chem., January 7, 2005; 280(1): 494 - 505. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. D. Moore, T. Voigt, Y. M. Kobayashi, G. Isenberg, F. S. Fay, M. F. Gallitelli, and C. Franzini-Armstrong Organization of Ca2+ Release Units in Excitable Smooth Muscle of the Guinea-Pig Urinary Bladder Biophys. J., September 1, 2004; 87(3): 1836 - 1847. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. D. Foell, R. C. Balijepalli, B. P. Delisle, A. M. R. Yunker, S. L. Robia, J. W. Walker, M. W. McEnery, C. T. January, and T. J. Kamp Molecular heterogeneity of calcium channel {beta}-subunits in canine and human heart: evidence for differential subcellular localization Physiol Genomics, April 13, 2004; 17(2): 183 - 200. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Yasuda, R. J. Lewis, and D. J. Adams Overexpressed Cav{beta}3 Inhibits N-type (Cav2.2) Calcium Channel Currents through a Hyperpolarizing Shift of "Ultra-slow" and "Closed-state" Inactivation J. Gen. Physiol., March 29, 2004; 123(4): 401 - 416. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Hummer, O. Delzeith, S. R. Gomez, R. L. Moreno, M. D. Mark, and S. Herlitze Competitive and Synergistic Interactions of G Protein {beta}2 and Ca2+ Channel {beta}1b Subunits with Cav2.1 Channels, Revealed by Mammalian Two-hybrid and Fluorescence Resonance Energy Transfer Measurements J. Biol. Chem., December 5, 2003; 278(49): 49386 - 49400. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Kobrinsky, E. Schwartz, D. R. Abernethy, and N. M. Soldatov Voltage-gated Mobility of the Ca2+ Channel Cytoplasmic Tails and Its Regulatory Role J. Biol. Chem., February 7, 2003; 278(7): 5021 - 5028. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Berrou, H. Klein, G. Bernatchez, and L. Parent A Specific Tryptophan in the I-II Linker Is a Key Determinant of beta -Subunit Binding and Modulation in CaV2.3 Calcium Channels Biophys. J., September 1, 2002; 83(3): 1429 - 1442. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. HOHAUS, V. PERSON, J. BEHLKE, J. SCHAPER, I. MORANO, and H. HAASE The carboxyl-terminal region of ahnak provides a link between cardiac L-type Ca2+ channels and the actin-based cytoskeleton FASEB J, August 1, 2002; 16(10): 1205 - 1216. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. E. Flucher, R. G. Weiss, and M. Grabner Cooperation of two-domain Ca2+ channel fragments in triad targeting and restoration of excitation- contraction coupling in skeletal muscle PNAS, July 23, 2002; 99(15): 10167 - 10172. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. S. Piedras-Renteria, K. Watase, N. Harata, O. Zhuchenko, H. Y. Zoghbi, C. C. Lee, and R. W. Tsien Increased Expression of alpha 1A Ca2+ Channel Currents Arising from Expanded Trinucleotide Repeats in Spinocerebellar Ataxia Type 6 J. Neurosci., December 1, 2001; 21(23): 9185 - 9193. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. D. Keef, J. R. Hume, and J. Zhong Regulation of cardiac and smooth muscle Ca2+ channels (CaV1.2a,b) by protein kinases Am J Physiol Cell Physiol, December 1, 2001; 281(6): C1743 - C1756. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Tateyama, S. Zong, T. Tanabe, and R. Ochi Properties of voltage-gated Ca2+ channels in rabbit ventricular myocytes expressing Ca2+ channel {alpha}1E cDNA Am J Physiol Cell Physiol, January 1, 2001; 280(1): C175 - C182. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. HAASE, T. PODZUWEIT, G. LUTSCH, A. HOHAUS, S. KOSTKA, C. LINDSCHAU, M. KOTT, R. KRAFT, and I. MORANO Signaling from {beta}-adrenoceptor to L-type calcium channel: identification of a novel cardiac protein kinase A target possessing similarities to AHNAK FASEB J, December 1, 1999; 13(15): 2161 - 2172. [Abstract] [Full Text]
|
|
|
|
|
|
S. Wittemann, M. D. Mark, J. Rettig, and S. Herlitze Synaptic Localization and Presynaptic Function of Calcium Channel beta 4-Subunits in Cultured Hippocampal Neurons J. Biol. Chem., November 22, 2000; 275(48): 37807 - 37814. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
