| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Colonic afferent fibres were recorded using a novel in vitro preparation. Fibres with endings in the colonic mucosa are described, along with those in muscle and serosa, and their responses to a range of mechanical and chemical luminal stimuli.
Mechanical stimuli were applied to the tissue, which included stretch, blunt probing of the mucosa and stroking of the mucosa with von Frey hairs (10-1000 mg). Chemical stimuli were applied into a ring that was placed over the mechanoreceptive field of the fibre; these were distilled water, 154 and 308 mM NaCl, 100 µM capsaicin, 50 mM HCl, and undiluted and 50 % ferret bile.
Recordings were made from 52 fibres, 12 of which showed characteristics of having endings in the mucosa. Mucosal afferents were sensitive to a 10 mg von Frey hair and were generally chemosensitive to 
1 chemical stimulus.
Ten fibres showed characteristics of having receptive fields in the muscular layer. These fibres responded readily to circumferential stretch, as well as to blunt probing.
Twenty-seven fibres showed characteristics of having endings in the serosal layer. They adapted rapidly to circumferential stretch and responded to blunt probing of the serosa. Fifteen of 19 serosal fibres tested also responded to luminal chemicals.
Three fibres were unresponsive to all mechanical stimuli but were recruited by chemical stimuli.
This is the first characterization of colonic afferent fibres using an in vitro method and the first documentation of afferent fibres with their endings in the mucosa of the colon. These fibres are likely to be important in aspects of colonic sensation and reflex control.
This article has been cited by other articles:
|
|
 |
|
  W. E. B. Sipe, S. M. Brierley, C. M. Martin, B. D. Phillis, F. B. Cruz, E. F. Grady, W. Liedtke, D. M. Cohen, S. Vanner, L. A. Blackshaw, et al. Transient receptor potential vanilloid 4 mediates protease activated receptor 2-induced sensitization of colonic afferent nerves and visceral hyperalgesia Am J Physiol Gastrointest Liver Physiol, May 1, 2008; 294(5): G1288 - G1298. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. P. Zagorodnyuk, I. L. Gibbins, M. Costa, S. J. H. Brookes, and S. J. Gregory Properties of the major classes of mechanoreceptors in the guinea pig bladder J. Physiol., November 15, 2007; 585(1): 147 - 163. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. M. Brunsden, S. J. H. Brookes, K. D. Bardhan, and D. Grundy Mechanisms underlying mechanosensitivity of mesenteric afferent fibers to vascular flow Am J Physiol Gastrointest Liver Physiol, August 1, 2007; 293(2): G422 - G428. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Tsukamoto, H. Ariga, C. Mantyh, T. N. Pappas, H. Yanagi, T. Yamamura, and T. Takahashi Luminally released serotonin stimulates colonic motility and accelerates colonic transit in rats Am J Physiol Regulatory Integrative Comp Physiol, July 1, 2007; 293(1): R64 - R69. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. R. Coldwell, B. D. Phillis, K. Sutherland, G. S. Howarth, and L. A. Blackshaw Increased responsiveness of rat colonic splanchnic afferents to 5-HT after inflammation and recovery J. Physiol., February 15, 2007; 579(1): 203 - 213. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Bielefeldt, F. Zhong, H. R. Koerber, and B. M. Davis Phenotypic characterization of gastric sensory neurons in mice Am J Physiol Gastrointest Liver Physiol, November 1, 2006; 291(5): G987 - G997. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M Million, L Wang, Y Wang, D W Adelson, P-Q Yuan, C Maillot, S V Coutinho, J A Mcroberts, A Bayati, H Mattsson, et al. CRF2 receptor activation prevents colorectal distension induced visceral pain and spinal ERK1/2 phosphorylation in rats Gut, February 1, 2006; 55(2): 172 - 181. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Wang, B. Tang, and R. J. Traub Differential Processing of Noxious Colonic Input by Thoracolumbar and Lumbosacral Dorsal Horn Neurons in the Rat J Neurophysiol, December 1, 2005; 94(6): 3788 - 3794. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. C. W. Jones III, L. Xu, and G. F. Gebhart The Mechanosensitivity of Mouse Colon Afferent Fibers and Their Sensitization by Inflammatory Mediators Require Transient Receptor Potential Vanilloid 1 and Acid-Sensing Ion Channel 3 J. Neurosci., November 23, 2005; 25(47): 10981 - 10989. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A J Page, S M Brierley, C M Martin, M P Price, E Symonds, R Butler, J A Wemmie, and L A Blackshaw Different contributions of ASIC channels 1a, 2, and 3 in gastrointestinal mechanosensory function Gut, October 1, 2005; 54(10): 1408 - 1415. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. M Brierley, R Carter, W Jones III, L. Xu, D. R Robinson, G. A Hicks, G. F Gebhart, and L. A. Blackshaw Differential chemosensory function and receptor expression of splanchnic and pelvic colonic afferents in mice J. Physiol., August 15, 2005; 567(1): 267 - 281. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Lynn, V. Zagorodnyuk, G. Hennig, M. Costa, and S. Brookes Mechanical activation of rectal intraganglionic laminar endings in the guinea pig distal gut J. Physiol., April 15, 2005; 564(2): 589 - 601. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M Costa, S H J Brookes, and V Zagorodnyuk How many kinds of visceral afferents? Gut, March 1, 2004; 53(90002): ii1 - 4. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. J. Page, C. M. Martin, and L. A. Blackshaw Vagal Mechanoreceptors and Chemoreceptors in Mouse Stomach and Esophagus J Neurophysiol, April 1, 2002; 87(4): 2095 - 2103. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Ozaki and G. F. Gebhart Characterization of mechanosensitive splanchnic nerve afferent fibers innervating the rat stomach Am J Physiol Gastrointest Liver Physiol, December 1, 2001; 281(6): G1449 - G1459. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. H. Percy, J. T. Brunz, R. E. Burgers, T. H. Fromm, C. L. Merkwan, and J. van Dis Interrelationship between colonic muscularis mucosae activity and changes in transmucosal potential difference Am J Physiol Gastrointest Liver Physiol, August 1, 2001; 281(2): G479 - G489. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H.-R. Berthoud, P. A. Lynn, and L. A. Blackshaw Vagal and spinal mechanosensors in the rat stomach and colon have multiple receptive fields Am J Physiol Regulatory Integrative Comp Physiol, May 1, 2001; 280(5): R1371 - R1381. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
