| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Mechanical stimuli are thought to modulate the number of sarcomeres in series (sarcomere number) in skeletal muscle fibres. However, the mechanisms by which muscle cells transduce mechanical signals into serial sarcomere addition have not been explored. In this study, we test the hypothesis that nitric oxide positively modulates sarcomere addition.
The soleus muscle was cast-immobilized in a shortened position in 3-week-old female Wistar rats. After 4 weeks, the casts were removed, creating a period of rapid sarcomere addition. During the remobilization period, nitric oxide synthase (NOS) inhibitors or substrate were administered.
Rats treated with the non-isoform-specific NOS inhibitor L-nitro-arginine methyl ester during 3 weeks of remobilization had smaller soleus sarcomere numbers than control rats. Rats treated with 1-(2-trifluoromethyl-phenyl)-imidazole, which has greater specificity for the neuronal isoform than for the endothelial isoform of NOS, also had smaller soleus sarcomere numbers than control rats. These results suggest that inhibition of the neuronal isoform of NOS reduces sarcomere addition during remobilization.
Rats treated with L-arginine, the substrate for NOS, during 1 week of remobilization had soleus sarcomere numbers for the immobilized-remobilized muscle which were closer to that for the contralateral, non-immobilized muscle than did rats that were not treated with L-arginine.
These results support the hypothesis that nitric oxide derived from the neuronal isoform of NOS positively modulates sarcomere addition.
This article has been cited by other articles:
|
|
 |
|
  D. Sumukadas MBBS MD, M. D. Witham MBChB PhD, A. D. Struthers MBChB MD, and M. E.T. McMurdo MBChB MD Effect of perindopril on physical function in elderly people with functional impairment: a randomized controlled trial Can. Med. Assoc. J., October 9, 2007; 177(8): 867 - 874. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. E. Sellman, K. C. DeRuisseau, J. L. Betters, V. A. Lira, Q. A. Soltow, J. T. Selsby, and D. S. Criswell In vivo inhibition of nitric oxide synthase impairs upregulation of contractile protein mRNA in overloaded plantaris muscle J Appl Physiol, January 1, 2006; 100(1): 258 - 265. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Wehling-Henricks, M. C. Jordan, K. P. Roos, B. Deng, and J. G. Tidball Cardiomyopathy in dystrophin-deficient hearts is prevented by expression of a neuronal nitric oxide synthase transgene in the myocardium Hum. Mol. Genet., July 15, 2005; 14(14): 1921 - 1933. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. S. Zhang, W. E. Kraus, and G. A. Truskey Stretch-induced nitric oxide modulates mechanical properties of skeletal muscle cells Am J Physiol Cell Physiol, August 1, 2004; 287(2): C292 - C299. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Barreiro, J. Gea, J. M. Corominas, and S. N. A. Hussain Nitric Oxide Synthases and Protein Oxidation in the Quadriceps Femoris of Patients with Chronic Obstructive Pulmonary Disease Am. J. Respir. Cell Mol. Biol., December 1, 2003; 29(6): 771 - 778. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. B. Reid Plasticity in Skeletal, Cardiac, and Smooth Muscle: Invited Review: Redox modulation of skeletal muscle contraction: what we know and what we don't J Appl Physiol, February 1, 2001; 90(2): 724 - 731. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. W. Smith, J. D. Smith, and D. S. Criswell Involvement of nitric oxide synthase in skeletal muscle adaptation to chronic overload J Appl Physiol, May 1, 2002; 92(5): 2005 - 2011. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
