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J Physiol Volume 520, Number 2, 417-429, October 15, 1999
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The Journal of Physiology (1999), 520.2, pp. 417-429
© Copyright 1999 The Physiological Society

Intracellular calcium signals measured with indo-1 in isolated skeletal muscle fibres from control and mdx mice

Claude Collet, Bruno Allard, Yves Tourneur and Vincent Jacquemond

Laboratoire de Physiologie des Eléments Excitables, CNRS UMR 5578, Université Claude Bernard Lyon 1, 43 boulevard du 11 Novembre 1918, 69622 Villeurbanne Cedex, France

  1. Intracellular free calcium concentration ([Ca2+]i) was measured with the fluorescent indicator indo-1 in single skeletal fibres enzymatically isolated from the flexor digitorum brevis and interosseus muscles of control and dystrophic mdx C57BL/10 mice. Measurements were taken from a portion of fibre that was voltage clamped to allow detection of depolarization-induced changes in [Ca2+]i.

  2. The mean (± s.e.m.) initial resting [Ca2+]i from all control and mdx fibres tested was 56 ± 5 nM (n = 72) and 48 ± 7 nM (n = 57), respectively, indicating no significant overall difference between the two groups. However, when comparing a batch of control and mdx fibres obtained from mice older than ~35 weeks, resting [Ca2+]i was significantly lower in mdx (16 ± 4 nM, n = 11) than in control fibres (71 ± 10 nM, n = 14).

  3. Changes in [Ca2+]i elicited by short (5-35 ms) depolarizing pulses from -80 to 0 mV showed similar properties in control and mdx fibres. After a 5 ms duration pulse the mean time constant of [Ca2+]i decay was, however, significantly elevated in mdx as compared to control fibres, by a factor of 1·5-2. For longer pulses, no significant difference could be detected.

  4. In response to 50 ms duration depolarizing pulses of various amplitudes the threshold for detection of an [Ca2+]i change and the peak [Ca2+]i reached for a given potential were similar in control and mdx fibres.

  5. Overall results show that mdx skeletal muscle fibres are quite capable of handling [Ca2+]i at rest and in response to membrane depolarizations.



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