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Flow modulates the transport of K⁺ through the walls of single perfused mesenteric venules in anaesthetised rats

M. Kajimura and C. C. Michel

1. We have investigated the effects of varying flow velocity (U) upon permeability to potassium ions (P_K) of single perfused mesenteric venules in anaesthetised rats. P_K was estimated using a development of the single bolus microperfusion technique at chosen flow velocities in the range of 300 to 6000 µm s^-1.

2. In an initial study on 12 vessels, there was a strong positive correlation between P_K and U. This was described by the relation:
   P_K = 0·0053U + 8·86,
   where P_K and U are both expressed in micrometres per second (µm s^-1).

3. The addition of the nitric oxide (NO) synthase inhibitors (20 µmol l^-1) N ^G-monomethyl-L-arginine (L-NMMA) and N ^G-nitro L-arginine (L-NNA) to the superfusate abolished the positive correlation between P_K and U. The addition of D-NNA (20 µmol l^-1) did not change the relation between P_K and U where the median value for the slope of the relation was 57·7 (± 58·7 interquartile (IQR)) × 10^-4 (n = 4). The addition of L-arginine (200 µmol l^-1) restored the relation between P_K and U where the slope of the relation was increased from 3·9 (± 16·3 IQR) × 10^-4 to 69·2 (± 13·5 IQR) × 10^-4 (n = 7).

4. The addition of the guanylate cyclase inhibitor LY83583 (10 µmol l^-1) abolished the positive correlation between P_K and U (n = 6).

5. Our data suggest that the flow modulates the potassium permeability through the walls of single perfused rat mesenteric venules via a NO-cGMP-dependent process.

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