HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kong, I. D. Articles by Sanders, K. M. Search for Related Content PubMed PubMed Citation Articles by Kong, I. D. Articles by Sanders, K. M.

Small conductance Ca²⁺-activated K⁺ channels are regulated by Ca²⁺-calmodulin-dependent protein kinase II in murine colonic myocytes

In Deok Kong, Sang Don Koh, Orline Bayguinov and Kenton M. Sanders

1. Ca²⁺ regulates the activity of small conductance Ca²⁺-activated K⁺ (SK) channels via calmodulin-dependent binding. We investigated whether other forms of Ca²⁺-dependent regulation might control the open probability of SK channels.

2. Under whole-cell patch-clamp conditions, spontaneous openings of SK channels can be resolved as charybdotoxin-insensitive spontaneous transient outward currents (STOCs). The Ca²⁺-calmodulin-dependent (CaM) protein kinase II inhibitor KN-93 reduced the occurrence of charybdotoxin-insensitive STOCs.

3. The charybdotoxin-insensitive STOCs are related to spontaneous, local release of Ca²⁺. KN-93 did not affect spontaneous Ca²⁺-release events.

4. KN-93 and W-7, a calmodulin inhibitor, decreased the open probability of SK channels in on-cell patches but not in excised patches.

5. Application of autothiophosphorlated CaM kinase II to the cytoplasmic surface of excised patches increased the open probalibity of SK channels. Boiled CaM kinase II had no effect.

6. We conclude that CaM kinase II regulates SK channels in murine colonic myocytes. This mechanism provides a secondary means of regulation, increasing the impact of a given Ca²⁺ transient on SK channel open probability.

This article has been cited by other articles:

				Y.-J. Qu, V. E. Bondarenko, C. Xie, S. Wang, M. S. Awayda, H. C. Strauss, and M. J. Morales W-7 modulates Kv4.3: pore block and Ca2+-calmodulin inhibition Am J Physiol Heart Circ Physiol, May 1, 2007; 292(5): H2364 - H2377. [Abstract] [Full Text] [PDF]

				M. Kim, I. S. Han, S. D. Koh, and B. A. Perrino Roles of CaM kinase II and phospholamban in SNP-induced relaxation of murine gastric fundus smooth muscles Am J Physiol Cell Physiol, August 1, 2006; 291(2): C337 - C347. [Abstract] [Full Text] [PDF]

				G. P. Sergeant, S. Ohya, J. A. Reihill, B. A. Perrino, G. C. Amberg, Y. Imaizumi, B. Horowitz, K. M. Sanders, and S. D. Koh Regulation of Kv4.3 currents by Ca2+/calmodulin-dependent protein kinase II Am J Physiol Cell Physiol, February 1, 2005; 288(2): C304 - C313. [Abstract] [Full Text] [PDF]

				E. A. Kramar, B. Lin, C.-Y. Lin, A. C. Arai, C. M. Gall, and G. Lynch A Novel Mechanism for the Facilitation of Theta-Induced Long-Term Potentiation by Brain-Derived Neurotrophic Factor J. Neurosci., June 2, 2004; 24(22): 5151 - 5161. [Abstract] [Full Text] [PDF]

				S. Ohya and B. Horowitz Differential transcriptional expression of Ca2+ BP superfamilies in murine gastrointestinal smooth muscles Am J Physiol Gastrointest Liver Physiol, December 1, 2002; 283(6): G1290 - G1297. [Abstract] [Full Text] [PDF]

				J. M. Lorenz, M. H. Riddervold, E. A. H. Beckett, S. A. Baker, and B. A. Perrino Differential autophosphorylation of CaM kinase II from phasic and tonic smooth muscle tissues Am J Physiol Cell Physiol, November 1, 2002; 283(5): C1399 - C1413. [Abstract] [Full Text] [PDF]

				O. Bayguinov, B. Hagen, J. L. Kenyon, and K. M. Sanders Coupling strength between localized Ca2+ transients and K+ channels is regulated by protein kinase C Am J Physiol Cell Physiol, November 1, 2001; 281(5): C1512 - C1523. [Abstract] [Full Text] [PDF]