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Properties of voltage-gated potassium currents in nucleated patches from large layer 5 cortical pyramidal neurons of the rat

John M. Bekkers

1. Voltage-gated potassium currents were studied in nucleated outside-out patches obtained from large layer 5 pyramidal neurons in acute slices of sensorimotor cortex from 13- to 15-day-old Wistar rats (22-25 °C).

2. Two main types of current were found, an A-current (I_A) and a delayed rectifier current (I_K), which were blocked by 4-aminopyridine (5 mM) and tetraethylammonium (30 mM), respectively.

3. Recovery from inactivation was mono-exponential (for I_A) or bi-exponential (for I_K) and strongly voltage dependent. Both I_A and I_K could be almost fully inactivated by depolarising prepulses of sufficient duration. Steady-state inactivation curves were well fitted by the Boltzmann equation with half-maximal voltage (V_½) and slope factor (k) values of -81·6 mV and -6·7 mV for I_A, and -66·6 mV and -9·2 mV for I_K. Peak activation curves were described by the Boltzmann equation with V_½ and k values of -18·8 mV and 16·6 mV for I_A, and -9·6 mV and 13·2 mV for I_K.

4. I_A inactivated mono-exponentially during a depolarising test pulse, with a time constant (~7 ms) that was weakly dependent on membrane potential. I_K inactivated bi-exponentially with time constants (~460 ms, ~4·2 s) that were also weakly voltage dependent. The time to peak of both I_A and I_K depended strongly on membrane potential. The kinetics of I_A and I_K were described by a Hodgkin-Huxley-style equation of the form m^Nh, where N was 3 for I_A and 1 for I_K.

5. These results provide a basis for understanding the role of voltage-gated potassium currents in the firing properties of large layer 5 pyramidal neurons of the rat neocortex.

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