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Distribution and activation of voltage-gated potassium channels in cell-attached and outside-out patches from large layer 5 cortical pyramidal neurons of the rat

John M. Bekkers

1. Voltage-gated potassium channels were studied in cell-attached and outside-out patches from the soma and primary apical dendrite of large layer 5 pyramidal neurons in acute slices of rat sensorimotor cortex (22-25 °C).

2. Ensemble averages revealed that some patches contained only fast, I_A-like channels, others contained only I_K-like channels that did not inactivate or inactivated slowly, and the remainder contained mixtures of both channel types. I_A and I_K channels had mean unitary conductances of 8·5 and 20·3 pS, respectively, and had distinctive patterns of gating.

3. Peak activation curves for ensemble-averaged currents were described by the Boltzmann equation with half-maximal voltage (V_½) and slope factor (k) values of -24·5 mV and 16·9 mV for I_A and -7·6 mV and 10·1 mV for I_K (patches < 250 µm from the soma) or -22·9 mV and 16·2 mV for I_A (patches > 250 µm from the soma). The steady-state inactivation curve for I_A gave V_½ and k values of -72·3 mV and -5·9 mV (< 250 µm from the soma) or -83·1 mV and -6·5 mV (> 250 µm from the soma). These values were similar to the corresponding data for I_A and I_K in nucleated patches from the same cell type.

4. The amount of I_A and I_K present in patches depended weakly on distance along the primary apical dendrite from the soma. The amplitude of I_A increased, on average, by 2·3 pA per 100 µm, while the amplitude of I_K decreased by 0·4 pA per 100 µm.

5. I_A and I_K channels in dendritic cell-attached patches were activated by the passage of a back-propagating action potential past the tip of the patch electrode. These results show directly that these potassium channels participate in action potential repolarisation, and thus contribute to the process of synaptic integration in these neurons.

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