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J Physiol Volume 527, Number 3, 493-506, September 15, 2000
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The Journal of Physiology (2000), 527.3, pp. 493-506
© Copyright 2000 The Physiological Society

A Kv3-like persistent, outwardly rectifying, Cs+-permeable, K+ current in rat subthalamic nucleus neurones

Mark A. Wigmore and Michael G. Lacey

Department of Pharmacology, Division of Neuroscience, The Medical School, University of Birmingham, Vincent Drive, Edgbaston, Birmingham B15 2TT, UK

  1. A persistent outward K+ current (IPO), activated by depolarization from resting potential, has been identified and characterized in rat subthalamic nucleus (SThN) neurones using whole-cell voltage-clamp recording in brain slices.

  2. IPO both rapidly activated (tau = 8 ms at +5 mV) and deactivated (tau = 2 ms at -68 mV), while showing little inactivation. Tail current reversal potentials varied with extracellular K+ concentration in a Nernstian manner.

  3. Intracellular Cs+ did not alter either IPO amplitude or the voltage dependence of activation, but blocked transient (A-like) outward currents activated by depolarization. When extracellular K+ was replaced with Cs+, IPO tail current reversal potentials were dependent upon the extracellular Cs+ concentration, indicating an ability to conduct Cs+, as well as K+.

  4. IPO was blocked by Ba2+ (1 mM), 4-aminopyridine (1 mM) and tetraethylammonium (TEA; 20 mM), with an IC50 for TEA of 0·39 mM.

  5. The IPO conductance appeared maximal (38 nS) at around +27 mV, half-maximal at -13 mV, with the threshold for activation at around -38 mV.

  6. TEA (1 mM) blocked the action potential after-hyperpolarization and permitted accommodation of action potential firing at frequencies greater than around 200 Hz.

  7. We conclude that IPO, which shares many characteristics of currents attributable to Kv3.1 K+ channels, enables high-frequency spike trains in SThN neurones.



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